Ab7821

Manufactured by Merck Group

The AB7821 is a laboratory equipment product manufactured by Merck Group. It is designed to perform basic laboratory tasks. The core function of the AB7821 is to facilitate standard laboratory procedures.

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Lab products found in correlation

710 confocal microscope, by Zeiss (3 mentions) Ab14511, by Abcam (1 mentions) Ab21286, by Abcam (1 mentions) Pa1 32130, by Thermo Fisher Scientific (1 mentions) Ab19808, by Abcam (1 mentions) Ab2413, by Abcam (1 mentions) Image lab software, by Bio-Rad (1 mentions) Chemidoc xrs system, by Bio-Rad (1 mentions) Lumi light, by Roche (1 mentions) Anti rabbit alexa fluor 647, by Thermo Fisher Scientific (1 mentions) Optimal cutting temperature compound o c t, by Sakura Finetek (1 mentions) His bond glass slides, by Avantor (1 mentions) Dmi6000 inverted microscope, by Zeiss (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Roche (1 mentions)

2 protocols using ab7821

Immunoblotting for Extracellular Matrix Proteins

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For immunoblotting, 100 μg protein of each exudate sample were separated under reducing conditions on 4–15% Tris–HCl polyacrylamide gradient gels, and transferred to nitrocellulose membranes. Immunodetection was performed by incubating the membranes overnight at 4°C stirring with rabbit anti-type IV collagen polyclonal antibody at a dilution of 1:200 (Abcam ab19808), rabbit anti-nidogen 1 polyclonal antibody at a dilution of 1:500 (Abcam ab14511), rabbit anti-laminin polyclonal antibody at a dilution of 1:1,000 (Thermo PA1-32130), rabbit anti-type VI collagen polyclonal antibody at a dilution of 1:2,000 (Millipore AB7821), rabbit anti-type I collagen polyclonal antibody at a dilution of 1:1,000 (Abcam ab21286), or rabbit anti-fibronectin polyclonal antibody at a dilution of 1:3,000 (Abcam ab2413). The reaction was developed using anti-rabbit peroxidase antibody at a dilution of 1:5,000 (Jackson ImmunoResearch) and the chemiluminescent substrate Lumi-Light (Roche). Images were captured with the ChemiDoc XRS+ System (BioRad) and the analysis was performed with the ImageLab software (BioRad).

Herrera C., Macêdo J.K., Feoli A., Escalante T., Rucavado A., Gutiérrez J.M, & Fox J.W. (2016). Muscle Tissue Damage Induced by the Venom of Bothrops asper: Identification of Early and Late Pathological Events through Proteomic Analysis. PLoS Neglected Tropical Diseases, 10(4), e0004599.

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Cryosectioning and Imaging of Limb Tissues

Cited 1 time

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Before cryosectioning, forelimbs were embedded in Optimal Cutting Temperature compound (OCT, Sakura Finetek), frozen with dry ice-cooled isopentane and stored at -80°C until sectioned. 5 – 10μm cryosections were collected on His-bond⁺ glass slides (VWR) and washed with PBS for 3 times to remove any residual OCT before conducting AFM. Cryosections were processed following (Calve et al., 2010 (link)) and stained with primary antibodies against perlecan (1:50; Santa Cruz Biotechnology sc33707) and type VI collagen (1:200, Millipore AB7821) and then the secondary detection reagents [Alexa Fluor 647 anti-rabbit (1:500, Life Technologies), DyLight 550 anti-rat (1:250, Pierce), DAPI (1:500, Roche). Sections were imaged at 10x using a Leica DMI6000 inverted microscope and at 63x using a Zeiss 710 confocal microscope. Pictures were acquired using the same imaging parameters across the different genotypes and processed under identical conditions using Adobe Photoshop.

Xu X., Li Z., Leng Y., Neu C.P, & Calve S. (2016). Knockdown of the pericellular matrix molecule perlecan lowers in situ cell and matrix stiffness in developing cartilage. Developmental biology, 418(2), 242-247.

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