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Faster prep fp120

Manufactured by Thermo Fisher Scientific
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The Faster Prep FP120 is a laboratory instrument designed for sample preparation. It is used to homogenize and disrupt samples, such as tissues or cells, in preparation for further analysis.

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Lab products found in correlation

Mo bio power fecal dna isolation kit, by Qiagen (4 mentions) Qubit fluromoter, by Thermo Fisher Scientific (4 mentions) Miseq instrument 300 bp kit, by Illumina (2 mentions) Miseq instrument, by Illumina (2 mentions) Powerfecal dna isolation kit, by Qiagen (1 mentions) Qubit fluorometer, by Thermo Fisher Scientific (1 mentions) Pippin prep 1.5 agarose cassette, by Sage Science (1 mentions)

5 protocols using faster prep fp120

1

16S rDNA V4 Amplicon Sequencing

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A multiplexed amplicon library covering the 16S rDNA gene V4 region was generated from human stool sample DNA as described 58 (link). Briefly, bacterial genomic DNA was extracted using the Mo Bio Power Fecal DNA Isolation kit (Mo Bio Laboratories) according to the manufacturer's instructions. To increase the DNA yields, the following modifications were used. An additional bead beater step using the Faster Prep FP120 (Thermo) at 6 meters/second for 1 min was used instead of vortex agitation. Incubation with buffers C2 and C3 was done for 10 min at 4°C. Starting nucleic acid concentrations were determined by a Qubit Fluromoter (Life Technologies). The amplicon library was prepared using dual-index barcodes 58 (link). The aggregated library pool was size selected from 300-500 base pairs (bp) on a pippin prep 1.5% agarose cassette (Sage Sciences) according to the manufacturer's instructions. The concentration of the pool was measured by qPCR (Kapa Biosystems) and loaded onto the MiSeq Illumina instrument (300 bp kit) at 6-9pM with 40% phiX spike-in to compensate for low base diversity according to Illumina's standard loading protocol.
Abdel-Gadir A., Stephen-Victor E., Gerber G.K., Rivas M.N., Wang S., Harb H., Wang L., Li N., Crestani E., Spielman S., Secor W., Biehl H., Dibendetto N., Dong X., Umetsu D.T., Bry L., Rachid R, & Chatila T.A. (2019). Microbiota Therapy Acts Via a Regulatory T Cell MyD88/RORγt Pathway to Suppress Food Allergy. Nature medicine, 25(7), 1164-1174.
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2

Stool DNA Extraction and 16S Sequencing

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Total genomic DNA was extracted from 500-mg stool samples using the PowerFecal DNA Isolation kit (Mo Bio Laboratories, Carlsbad, CA, USA), according to the manufacturer’s instructions with the following modifications to improve yields from difficult-to-lyse bacteria. An additional bead-beating step using Faster Prep FP120 (Thermo) at 6 m/s for 1 min was used instead of vortex agitation. Incubation with buffers C2 and C3 was increased to 10 min at 4 °C. Subsequently, quantity of extracted DNA samples were measured by a Qubit Fluorometer (Life Technologies, Carlsbad, CA, USA), and then, extracted DNA of samples were sent to MIT-BioMicroCenter for multiplexed amplicon library preparation, covering the 16S rRNA gene V4 region using a dual-index barcode protocol, followed by Illumina MiSeq 16S rRNA gene sequencing.
Pakpour S., Bhanvadia A., Zhu R., Amarnani A., Gibbons S.M., Gurry T., Alm E.J, & Martello L.A. (2017). Identifying predictive features of Clostridium difficile infection recurrence before, during, and after primary antibiotic treatment. Microbiome, 5, 148.
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3

Profiling Gut Microbiome via 16S rDNA

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A multiplexed amplicon library covering the 16S rDNA gene V4 region was generated from human stool sample DNA. Bacterial genomic DNA was extracted using the Mo Bio Power Fecal DNA Isolation kit (Mo Bio Laboratories, Carlsbad, CA) according to the manufacturer’s instructions for high yields of DNA with the following modifications to increase yields from difficult to lyse microbes. An additional bead beater step using the Faster Prep FP120 (Thermo) at 6 meters/second for 1 min was used instead of vortex agitation. Incubation with buffers C2 and C3 was increased to 10 min at 4°C.
Starting nucleic acid concentrations were determined by a Qubit Fluromoter (Life Technologies, Carlsbad, CA). A multiplexed amplicon library covering the 16S rDNA gene V4 region was prepared using the protocol of with dual-index barcodes.17 (link) The aggregated library pool was size selected from 300–500 bp on a pippin prep 1.5% agarose cassette (Sage Science, Beverly, MA) according to the manufacturer’s instructions. The concentration of the pool was measured by qPCR (Kapa Biosystems, Willmington, MA) and loaded onto the MiSeq Illumina instrument (300 bp kit) at 6–9pM with 40% phiX spike-in to compensate for low base diversity according to Illumina’s standard loading protocol.
Allegretti J.R., Kearney S., Li N., Bogart E., Bullock K., Gerber G.K., Bry L., Clish C.B., Alm E, & Korzenik J. (2016). Recurrent Clostridium difficile Infection Associates with Distinct Bile Acid and Microbiome Profiles. Alimentary pharmacology & therapeutics, 43(11), 1142-1153.
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4

Bacterial DNA Extraction and 16S Amplicon Sequencing

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Bacterial genomic DNA was extracted using the Mo Bio Power Fecal DNA Isolation kit (Mo Bio Laboratories) according to the manufacturer’s instructions for high yields of DNA. To increase the DNA yields, the following modifications were used. An additional bead beater step using the Faster Prep FP120 (Thermo) at 6 m/s for 1 min was used instead of vortex agitation. Incubation with buffers C2 and C3 was increased to 10 min at 4 °C. Starting nucleic acid concentrations were determined by a Qubit Fluromoter (Life Technologies). A multiplexed amplicon library covering the 16S rDNA gene V4 region was prepared using the protocol of [53 (link)] with dual-index barcodes. The aggregated library pool was size selected from 300–500 bp on a pippin prep 1.5 % agarose cassette (Sage Sciences) according to the manufacturer’s instructions. The concentration of the pool was measured by qPCR (Kapa Biosystems) and loaded onto the MiSeq Illumina instrument (300 bp kit) at 6–9 pM with 40 % phiX spike-in to compensate for low base diversity according to Illumina’s standard loading protocol. Sequences were deposited in the NIH Sequence Read Archive (SRA accession SRP065075).
Bucci V., Tzen B., Li N., Simmons M., Tanoue T., Bogart E., Deng L., Yeliseyev V., Delaney M.L., Liu Q., Olle B., Stein R.R., Honda K., Bry L, & Gerber G.K. (2016). MDSINE: Microbial Dynamical Systems INference Engine for microbiome time-series analyses. Genome Biology, 17, 121.
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5

16S rDNA V4 Amplicon Sequencing

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
A multiplexed amplicon library covering the 16S rDNA gene V4 region was generated from human stool sample DNA as described 58 (link). Briefly, bacterial genomic DNA was extracted using the Mo Bio Power Fecal DNA Isolation kit (Mo Bio Laboratories) according to the manufacturer's instructions. To increase the DNA yields, the following modifications were used. An additional bead beater step using the Faster Prep FP120 (Thermo) at 6 meters/second for 1 min was used instead of vortex agitation. Incubation with buffers C2 and C3 was done for 10 min at 4°C. Starting nucleic acid concentrations were determined by a Qubit Fluromoter (Life Technologies). The amplicon library was prepared using dual-index barcodes 58 (link). The aggregated library pool was size selected from 300-500 base pairs (bp) on a pippin prep 1.5% agarose cassette (Sage Sciences) according to the manufacturer's instructions. The concentration of the pool was measured by qPCR (Kapa Biosystems) and loaded onto the MiSeq Illumina instrument (300 bp kit) at 6-9pM with 40% phiX spike-in to compensate for low base diversity according to Illumina's standard loading protocol.
Abdel-Gadir A., Stephen-Victor E., Gerber G.K., Rivas M.N., Wang S., Harb H., Wang L., Li N., Crestani E., Spielman S., Secor W., Biehl H., Dibendetto N., Dong X., Umetsu D.T., Bry L., Rachid R, & Chatila T.A. (2019). Microbiota Therapy Acts Via a Regulatory T Cell MyD88/RORγt Pathway to Suppress Food Allergy. Nature medicine, 25(7), 1164-1174.
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