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Secondary abs

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Secondary Abs are laboratory reagents used in various immunoassay techniques. They function as detection antibodies, binding to primary antibodies and enabling the visualization or quantification of target analytes. Secondary Abs provide a versatile and sensitive method for detecting and analyzing a wide range of biomolecules.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Deoxyribonuclease 1, by Merck Group (2 mentions) Hematoxylin, by Merck Group (2 mentions) Phosphate buffered saline tablet, by Merck Group (2 mentions) Nkp46 m20, by Santa Cruz Biotechnology (2 mentions) Penicillin g, by Thermo Fisher Scientific (2 mentions) Hoechst, by Thermo Fisher Scientific (2 mentions) Percoll, by Merck Group (2 mentions) Rabbit anti iba1, by Fujifilm (2 mentions) Glucose, by Merck Group (2 mentions) Cxcl12, by Thermo Fisher Scientific (1 mentions) Recombinant human egf, by Thermo Fisher Scientific (1 mentions) Rt pcr system, by Thermo Fisher Scientific (1 mentions) Bovine serum albumine (bsa), by Merck Group (1 mentions) Ifn γ, by Immunological Sciences (1 mentions) Microbeads cd11b, by Miltenyi Biotec (1 mentions) Transwell insert, by BD (1 mentions) Lipopolysaccharides (lps), by Immunotools (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Goat serum, by Thermo Fisher Scientific (1 mentions)

2 protocols using secondary abs

1

Isolation and Analysis of Microglia

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All Culture media, fetal bovine serum (FBS), goat serum, penicillin G, streptomycin, glutamine, Na pyruvate, recombinant human EGF, Thermo Script RT‐PCR System, anti‐Acetyl‐alpha Tubulin (6‐11B‐1), anti‐Acetyl‐alpha Tubulin lys40 Abs and Hoechst (#33342, RRID:AB_10626776) were from GIBCO Invitrogen (Carlsbad, CA). Glucose, hematoxylin, eosin, Percoll, Papain (#P3125), phosphate buffered‐saline (PBS) tablet (#P4417), Bovine Serum Albumine (BSA) and deoxyribonuclease I were from Sigma‐Aldrich (Milan, Italy). Transwell inserts were from BD Labware (Franklin Lakes, NJ). Ki67 (#12202, RRID:AB 2620142) Ab were from Cell Signaling (Danvers, MA). NKp46 (M20) (#sc‐18,161, RRID: AB_2149152) antibody (Ab) was from Santa Cruz biotechnology (Santa Cruz, CA). Anti‐alpha tubulin (ab52866) and anti‐TMEM119 Abs were from Abcam (Cambridge, UK). Secondary Abs were from DAKO (Milan, Italy). CXCL12 was from Peprotech. LPS is from Immunotools (Friesoythe, Germany). IFN‐γ were from Immunological Sciences (Rome, Italy). Microbeads CD11b+ were from Miltenyi Biotec (Bologna, Italy). CD45, CD69, CD107a, NK1.1 Abs were from eBioscience Inc. (San Diego, CA). Rabbit anti‐Iba1 from Wako (VA).
Mormino A., Cocozza G., Fontemaggi G., Valente S., Esposito V., Santoro A., Bernardini G., Santoni A., Fazi F., Mai A., Limatola C, & Garofalo S. (2021). Histone‐deacetylase 8 drives the immune response and the growth of glioma. Glia, 69(11), 2682-2698.
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2

Isolation and Characterization of Mouse NK Cells

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All culture media, fetal bovine serum (FBS), goat serum, penicillin G, streptomycin, glutamine, Na pyruvate, ThermoScript RT-PCR Systems, Hoechst (#33342, RRID : AB_10626776), and mouse anti-IL-15 (PA5-47014) were from Gibco Invitrogen (Carlsbad, CA, USA). Glucose, hematoxylin, eosin, Percoll, Papain (#P3125), phosphate buffered-saline (PBS) tablet (#P4417), bovine serum albumin (BSA), and deoxyribonuclease I were from Sigma-Aldrich (Milan, Italy). NKp46 (M20) (#sc-18161, RRID: AB_2149152) antibody (Ab) and Arg1 (E-2) (#sc-271430) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Secondary Abs were from DAKO (Milan, Italy). Rabbit anti-Iba1 was from Wako (VA, USA). Anti-mouse (clone name in brackets) CD45 (104), CD69 (H1.2F3), CD3 (145-2C11), NKG2D (CX5), and NK1.1 (Pk136) mAbs directly conjugated to FITC, PE, PerCP 5.5, allophycocyanin, and allophycocyanin-eFluor 780 were from Ebioscences (Thermo Fisher Scientific, Waltham, MA USA). The pAAV-CMV-IL-15-GFP vector (CW304678) expressing the mouse IL-15 ORF under the control of a Cytomegalovirus promoter was from Tema Ricerca (Bologna, Italy).
Mormino A., Bernardini G., Cocozza G., Corbi N., Passananti C., Santoni A., Limatola C, & Garofalo S. (2021). Enriched Environment Cues Suggest a New Strategy to Counteract Glioma: Engineered rAAV2-IL-15 Microglia Modulate the Tumor Microenvironment. Frontiers in Immunology, 12, 730128.
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