Methylated lambda bacteriophage gdna

HeLa-S3, IMR-90, and K562 gDNAs were purchased from MilliporeSigma (catalog #87110901, 85020204, 89121407). GM12878 gDNA was purchased from the Coriell Institute for Medical Research (#NA12878). Methylated lambda bacteriophage gDNA, from strain cI857 Sam7 grown in E. coli strain W3110, was purchased from Thermo Fisher Scientific (#SD0011). Unmethylated lambda gDNA, from the same virus strain grown in dam⁻dcm⁻E. coli strain GM2163, was purchased from Thermo Fisher Scientific (#SD0021). All gDNA samples were requantified with a Qubit 1X dsDNA High-Sensitivity kit (#Q33230; Thermo Fisher Scientific) and diluted according to this measurement in nuclease-free 1X TE buffer with 0.05% wt/vol Tween 20. All experiments used 60 ng gDNA except those comparing lower inputs, for which gDNA was serially diluted and technical replicates were taken from the same dilution.

HeLa-S3, IMR-90, and K562 gDNAs were purchased from MilliporeSigma (catalog #87110901, 85020204, 89121407). GM12878 gDNA was purchased from the Coriell Institute for Medical Research (#NA12878). Methylated lambda bacteriophage gDNA, from strain cI857 Sam7 grown in E. coli strain W3110, was purchased from Thermo Fisher Scientific (#SD0011). Unmethylated lambda gDNA, from the same virus strain grown in dam⁻dcm⁻E. coli strain GM2163, was purchased from Thermo Fisher Scientific (#SD0021). All gDNA samples were requantified with a Qubit 1X dsDNA High Sensitivity kit (Thermo Fisher Scientific #Q33230) and diluted according to this measurement in nuclease-free 1X TE buffer with 0.05% w/v Tween 20. All experiments used 60ng gDNA except those comparing lower inputs, for which gDNA was serially diluted and technical replicates were taken from the same dilution.