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Apc anti human cd45 antibody

Manufactured by BioLegend
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Sourced in China

The APC anti-human CD45 antibody is a fluorescently-labeled monoclonal antibody that specifically binds to the CD45 antigen expressed on the surface of human leukocytes. CD45 is a transmembrane protein tyrosine phosphatase that is involved in the regulation of T-cell and B-cell antigen receptor signaling.

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Lab products found in correlation

Pe mouse anti human cd9, by BD (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Sony (2 mentions) Sh800 cell sorter, by Sony (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by BD (2 mentions) Cd326 epcam microbeads, by Miltenyi Biotec (1 mentions)

Close spelling variants of this product

APC-conjugated anti-human CD45 antibody Anti-human CD45 APC antibody (clone HI30, APC anti-human CD45 Anti-human CD45 antibody Anti-CD45-APC Human CD45 antibody Anti-human CD45 Anti-CD45 antibody CD45-APC Anti-CD45

3 protocols using apc anti human cd45 antibody

1

NK Cell Phenotyping by CD9 Expression

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OVCAR4 cells were cocultured with NK-92 or NKL cells at a 1:1 ratio
in U-bottom 96 well plates for 24h at 37°C. Cocultured cells were
harvested and washed with serum-free RPMI-1640 medium. Cells (2 ×
106) were stained in 90 μL of serum-free RPMI-1640
medium with 5 μL of PE mouse anti-human CD9 (BD PharMingen, clone
M-L13) and 5 μL of APC anti-human CD45 antibody (BioLegend, clone
HI30) for 30min on ice. The cells were washed with serum-free RPMI-1640
medium and stained with DAPI (BD PharMingen, 0.1 μg/mL) in 1mL of
serum-free RPMI-1640 medium for 10min at RT, quenched and washed with
complete RPMI-1640 media (supplemented with 10% FBS, 2 mM L-glutamine and 1%
pen strep). Cells were resuspended in 1mL complete RPMI-1640 media and
filtered before sorting. CD9+ and CD9− NK cells were collected in
FBS. Cells grown in monoculture were used as controls.
Cells were sorted on a Sony SH800 cell sorter, according to the
following steps: i) FSC- versus FSC-A for singlets ii) DAPI for live cells
iii) CD45 for NK cells and iv) CD9 versus CD45 to collect CD9+ and
CD9− NK cells.
Gonzalez V.D., Huang Y.W., Delgado-Gonzalez A., Chen S.Y., Donoso K., Sachs K., Gentles A.J., Allard G.M., Kolahi K.S., Howitt B.E., Porpiglia E, & Fantl W.J. (2021). High-grade serous ovarian tumor cells modulate NK cell function to create an immune-tolerant microenvironment. Cell reports, 36(9), 109632.
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2

Glypican-3 Detection in Cells

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Fe3O4 solution (5mg/mL) was purchased from Xi’an ruixi Biological Technology Co., Ltd. Mal-PEG2000-COOH was obtained from AVT (Shanghai) Pharmaceutical Tech Co., Ltd. Anti-human Glypican-3 antibody was purchased from Abcepta Biotech Ltd. Co. APC anti-human CD45 antibody was purchased from Biolegend (DAKEWE, Shenzhen, China). PE anti-human CK18 were purchased from Biosynthesis Biotechnology Inc. (Beijing, China). CD326 (EpCAM) MicroBeads were purchased from Miltenyi. And other reagents and solvents were obtained from Sinopharm Co., Ltd (Shanghai, China) and Sigma-Aldrich Co., Ltd (Shanghai, China).
Chu Q., Mu W., Lan C., Liu Y., Gao T., Guan L., Fang Y., Zhang Z., Liu Y., Liu Y, & Zhang N. (2021). High-Specific Isolation and Instant Observation of Circulating Tumour Cell from HCC Patients via Glypican-3 Immunomagnetic Fluorescent Nanodevice. International Journal of Nanomedicine, 16, 4161-4173.
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3

NK Cell Phenotyping by CD9 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
OVCAR4 cells were cocultured with NK-92 or NKL cells at a 1:1 ratio
in U-bottom 96 well plates for 24h at 37°C. Cocultured cells were
harvested and washed with serum-free RPMI-1640 medium. Cells (2 ×
106) were stained in 90 μL of serum-free RPMI-1640
medium with 5 μL of PE mouse anti-human CD9 (BD PharMingen, clone
M-L13) and 5 μL of APC anti-human CD45 antibody (BioLegend, clone
HI30) for 30min on ice. The cells were washed with serum-free RPMI-1640
medium and stained with DAPI (BD PharMingen, 0.1 μg/mL) in 1mL of
serum-free RPMI-1640 medium for 10min at RT, quenched and washed with
complete RPMI-1640 media (supplemented with 10% FBS, 2 mM L-glutamine and 1%
pen strep). Cells were resuspended in 1mL complete RPMI-1640 media and
filtered before sorting. CD9+ and CD9− NK cells were collected in
FBS. Cells grown in monoculture were used as controls.
Cells were sorted on a Sony SH800 cell sorter, according to the
following steps: i) FSC- versus FSC-A for singlets ii) DAPI for live cells
iii) CD45 for NK cells and iv) CD9 versus CD45 to collect CD9+ and
CD9− NK cells.
Gonzalez V.D., Huang Y.W., Delgado-Gonzalez A., Chen S.Y., Donoso K., Sachs K., Gentles A.J., Allard G.M., Kolahi K.S., Howitt B.E., Porpiglia E, & Fantl W.J. (2021). High-grade serous ovarian tumor cells modulate NK cell function to create an immune-tolerant microenvironment. Cell reports, 36(9), 109632.
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