Anti e cadherin monoclonal antibody

The following primary monoclonal and polyclonal antibodies were used to detect cytoskeletal and FA proteins by immunofluorescent labeling and immunoblot analysis: anti-GAPDH monoclonal antibody (Cat. No. ab8245, Abcam, Cambridge, MA), anti-secretoglobin family 1A member 1 (CC10/scgb1a1; Cat. No. 213202, Abcam), anti-E-cadherin monoclonal antibody (Cat. No. 610181, BD Bioscience, San Jose, CA), total paxillin (PAX; Cat. No. 2542, Cell Signaling, Danvers, MA), phospho-PAX Tyr¹¹⁸ (pPAX; Cat. No. 2541, Cell Signaling), total focal adhesion kinase (FAK; Cat. No. 3285, Cell Signaling), phospho-FAK Tyr³⁹⁷ (pFAK, Cat. No. 3283, Cell Signaling), phospho-MLC2 (pMLC2; Cat. No. 3671t, Cell Signaling), MLC2 (Cat. No. 8505s, Cell Signaling), and RhoA (Cat. No. 2117s, Cell Signaling). Alexa Fluor 488 (Cat. No. A12379) and 633 (Cat. No. A22284) phalloidin as well as anti-rabbit and anti-mouse secondary antibodies conjugated to Alexa Fluor 488, 568, or 633 (Cat. Nos. A-21206, A-10042, and A-21050) dyes were obtained from Thermo Fisher Scientific (Waltham, MA). The specificity of antibodies against MLC2 and pMLC2 has been reported previously (49 (link)–51 (link)). ROCK inhibitor Y-27632 was purchased from Millipore Sigma (Cat. No. SCM075, St. Louis, MO) and resuspended in deionized water. Y-27632 inhibits both ROCK1 and ROCK2 by competing with ATP for binding to the catalytic site (52 (link)).