Q rt pcr and sds page experiments

CaCl₂ was purchased from Bodi Chemical Co., Ltd. (Tianjin, China). Antibodies specific against NeuN and Alexa Fluor-488, Alexa Fluor-555, and HRP-labeled secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, United States). S(+)-Ketamine (60 mg/kg, for 1 h), SC-51322 (30 nM, for 12 h), PF-04418948 (100 nM, for 12 h), and DG-041 (60 nM, for 12 h) were obtained from R&D Systems (Minneapolis, MN, United States), and CJ-42794 (40 nM, for 12 h) was obtained from MedChem Express (Monmouth Junction, NJ, United States). High-fidelity (HF) restriction enzymes for EcoRI, BamHI, XhoI, and AgeI were purchased from New England Biolabs (Beverly, MA, United States). DAPI was procured from Beyotime Institute of Biotechnology (Haimen, China). The plko.1-puro, psPAX₂, pMD₂.G, and plvx-IRES-zsgreen vectors were purchased from Addgene (Sidney, SD, United States). All the reagents used for the quantitative (q)RT-PCR and SDS-PAGE experiments were purchased from Bio-Rad Laboratories (Hercules, CA, United States), and all other reagents were obtained from Invitrogen (Carlsbad, CA, United States), unless otherwise specified.

Indomethacin was purchased from Chengdu Aikeda Chemical Reagent Co., Ltd. (Chengdu, Sichuan, China). Bay-u3405 was obtained from Adooq Bioscience (Nanjing, Jiangsu, China). Human Aβ_1–42 was purchased from ChinaPeptides (Suzhou, Jiangsu, China). PGD₂ was purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). Antibodies against β-actin, LRP1, Aβ, ADAM10, p-AKT, AKT, p-ERK1/2, ERK1/2, p-mTOR, p-TSC2, p-4EBP1 and p-S6K were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). Antibodies specific for COX-2 were purchased from Abcam (Shanghai, China). Antibodies specific for A2M, L-PGDS and CRTH2 were purchased from Santa Cruz Biotechnology (Shanghai, China). All reagents used in the qRT-PCR and SDS-PAGE experiments were purchased from Bio-Rad Laboratories (Shanghai, China). All other reagents were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA) unless specified otherwise.