6-Benzylaminopurine (BA), 1-naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA), kinetin (KIN), Murashige and Skoog (MS) basal medium, and mercury (II) chloride (HgCl2), were acquired from Sigma-Aldrich, Germany. Dimethyl sulfoxide, acetone, and additional solvents and reagents were bought from VWR International, Belgium.
Mercury 2 chloride hgcl2
Manufactured by Merck Group
Sourced in Germany, United States
Mercury (II) chloride (HgCl2) is an inorganic compound consisting of one mercury atom and two chlorine atoms. It is a white, crystalline solid that is soluble in water and other polar solvents.
Automatically generated - may contain errors
Lab products found in correlation
Acetone, by Avantor (1 mentions)
Kinetin, by Merck Group (1 mentions)
Indole 3 acetic acid, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Avantor (1 mentions)
Murashige and skoog, by Merck Group (1 mentions)
6 benzylaminopurine, by Merck Group (1 mentions)
1 naphthaleneacetic acid, by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Phytic acid, by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid disodium salt dihydrate, by Merck Group (1 mentions)
Rhodamine b, by Merck Group (1 mentions)
Triethylenetetramine teta, by Merck Group (1 mentions)
1 vinyl 2 pyrrolidone nvp, by Merck Group (1 mentions)
Dichloromethane dcm, by Merck Group (1 mentions)
N n methylenebis acrylamide mba, by Merck Group (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Ethanol etoh, by Merck Group (1 mentions)
D8418, by Merck Group (1 mentions)
Slgp033rs, by Merck Group (1 mentions)
W4502, by Merck Group (1 mentions)
4 protocols using mercury 2 chloride hgcl2
1
Plant Growth Regulation: A Comprehensive Protocol
2
Nanoparticle Synthesis and Characterization
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Titanium dioxide NPs (CAS No. 13463-67-7; #700347; <150 nm—anatase/rutile ca. 80:20) and cerium oxide NPs (CAS No. 1306-38-3; #643009; <25 nm) were purchased from Sigma-Aldrich. Sodium meta-arsenite (NaAsO2, ≥90%, CAS No. 7784-465-56) and mercury (II) chloride (HgCl2; CAS No. 7487-94-7) were purchased from Sigma-Aldrich and Merck, respectively. Nitric (HNO3 65%; CAS No. 7697-37-2) and fluoridric (HF 40%; CAS No. 7664-39-3) acids were both from Merck. H2O2 (CAS No. 7722-84-1) and Triton X-100 (CAS No. 9002-93-1) were bought from Sigma-Aldrich (St. Louis, MO, USA).
3
Hydrogel-Based Heavy Metal Sequestration
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Poly(vinyl alcohol) (PVA, average Mw 85,000–124,000 Da, 99%+ hydrolysis degree, Sigma Aldrich, Saint Louis, MO, USA), 2-Acrylamido-2-methyl-1-propanesulfonic acid (AMPSA, 99%, Sigma Aldrich, Saint Louis, MO, USA), N,N′-methylenebisacrylamide (MBA, 99%, Sigma Aldrich, Saint Louis, MO, USA), 2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Ph-In, 98%, Sigma-Aldrich, Saint Louis, MO, USA), rhodamine B (RB, analytical standard, Sigma-Aldrich, Saint Louis, MO, USA), triethylenetetramine (TETA, Sigma-Aldrich, Saint Louis, MO, USA), ethanol (EtOH, Sigma-Aldrich, Saint Louis, MO, USA), dichloromethane (DCM, ≥99.8%, Sigma-Aldrich, Saint Louis, MO, USA), Phytic acid (Phytic acid, sodium salt hydrate from rice, Sigma-Aldrich, Saint Louis, MO, USA), 1,3-diamino-2-hydroxypropane-N,N,N′,N′-tetraacetic acid (TETRA, 99%, Sigma-Aldrich, Saint Louis, MO, USA), triethylenetetramine-N,N,N′,N″,N‴,N″″-hexaacetic acid (HEXA, ≥98.0%, Sigma-Aldrich, Saint Louis, MO, USA), ethylenediaminetetraacetic acid (EDTA, ethylenediaminetetraacetic acid disodium salt dihydrate, 99.0%, Sigma-Aldrich, Saint Louis, MO, USA), mercury(II) chloride (HgCl2, 99%, Sigma-Aldrich, Saint Louis, MO, USA), and deionized water were used as received. 1-vinyl-2-pyrrolidone (NVP, sodium hydroxide inhibitor, ≥99%, Sigma Aldrich, Saint Louis, MO, USA) was distilled and stored at 4 °C before the synthesis steps.
4
Plasmid Cloning and Genetic Circuit Characterization
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Plasmid cloning and in vivo genetic
circuit characterization were all performed
in E. coli TOP10. Cells were cultured in Lysogeny
broth (LB) medium (10 g L–1 peptone, 5 g L–1 NaCl, 5 g L–1 yeast extract), with appropriate
antibiotics. The antibiotic concentrations used were 50 μg mL–1 for both kanamycin and ampicillin (for low copy number
plasmid) or 100 μg mL–1 for ampicillin (for
high copy number plasmid). Antibiotics and inducers (i.e., mercury(II)
chloride (HgCl2) and N-(β-ketocaproyl)-l -homoserine lactone (3OC6HSL)) were analytical grade
and purchased from Sigma-Aldrich. They were dissolved in ddH2O or nuclease free H2O (W4502, Sigma-Aldrich) and were
then filtered using 0.22 μm syringe filters (SLGP033RS, Millipore).
LacZ substrate 5-bromo-4-chloro-3-indolyl β-d -galactopyranoside
(X-gal) (MB1001, Melford) was dissolved in dimethyl sulfoxide (DMSO)
(D8418, Sigma-Aldrich) to make 2% or 5% (w/v) stock solutions. Substrate
furimazine for NanoLuc luciferase was from Nano-Glo Luciferase Assay
System (N1110, Promega).
circuit characterization were all performed
in E. coli TOP10. Cells were cultured in Lysogeny
broth (LB) medium (10 g L–1 peptone, 5 g L–1 NaCl, 5 g L–1 yeast extract), with appropriate
antibiotics. The antibiotic concentrations used were 50 μg mL–1 for both kanamycin and ampicillin (for low copy number
plasmid) or 100 μg mL–1 for ampicillin (for
high copy number plasmid). Antibiotics and inducers (i.e., mercury(II)
chloride (HgCl2) and N-(β-ketocaproyl)-
and purchased from Sigma-Aldrich. They were dissolved in ddH2O or nuclease free H2O (W4502, Sigma-Aldrich) and were
then filtered using 0.22 μm syringe filters (SLGP033RS, Millipore).
LacZ substrate 5-bromo-4-chloro-3-indolyl β-
(X-gal) (MB1001, Melford) was dissolved in dimethyl sulfoxide (DMSO)
(D8418, Sigma-Aldrich) to make 2% or 5% (w/v) stock solutions. Substrate
furimazine for NanoLuc luciferase was from Nano-Glo Luciferase Assay
System (N1110, Promega).
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