Blood glucose monitoring system

FGM was used to monitor interstitial fluid glucose levels, and only the 5-hour postprandial period FGMS data were utilized for analysis. Aside from FGMS data, capillary blood glucose levels were assessed using the Abbott blood glucose monitoring system at the beginning of the meals (T = 0), 120 min after meals, and when symptomatic hypoglycemia occurred.
FGMS measurements yielded the following outcome parameters: (1) mean glucose levels, which were recorded every 15 min with the FGMS; (2) peak glucose level, which was the highest level recorded during the 5-h postprandial period; the time of its occurrence was used to determine the time to peak glucose; (3) incremental area under the glucose excursion curve, which was determined as the area under the glucose curve during the 5-hour postprandial period with the glucose level at T = 0 as the baseline; (4) hypoglycemic episodes, which were defined as glucose levels less than 3.9 mmol/L measured by FGMS, at which time the onset of hypoglycemia was recorded; (5) time above range (> 10 mmol/L) during the 5-hour postprandial period; and (6) glucose excursions, which were defined as variations in glucose levels measured every thirty minutes.

As previous report [9 (link)], a diabetic mouse model was generated by feeding a high-fat diet for 3 weeks, followed by streptozotocin (STZ, Sigma, USA) intraperitoneal injection at a dose of 100 mg/kg (dissolved in 0.1 mol/L sodium citrate solution to a final concentration of 10 g/L pH adjusted to 4.2–4.3). The high-fat diet was preceded for 4 weeks before the MCAO surgery. On day 50, all mice fast for 6 to 8 hr and their blood glucose levels from tails were analyzed using a blood glucose monitoring system (Abbott Leeshai, China). Taken the fasting, blood glucose ≥ 10 mmol/L was considered the success of diabetic mouse model establishment. For concerning the aging problem, we have set up the control group which was fed a normal diet and was injected with vehicle for STZ and was raised in the same environment. A trend in blood glucose and weight of both groups of mice is shown in Figure 1(a). Mice that died less than 28 days after STZ or vehicle injection were excluded.