C maf

Manufactured by BD

The C-Maf is a laboratory equipment product manufactured by BD. It is a device designed for the detection and measurement of specific cellular components or analytes within a sample. The core function of the C-Maf is to facilitate analytical procedures, providing researchers and scientists with a tool to support their investigations.

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2 protocols using c maf

Multiparametric Immune Cell Profiling

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Specific cell-surface staining was performed using a standard procedure with anti-CD4, anti-PD1, anti-IgD, anti-GL7 (eBioscience), and anti-CXCR5 mAbs (BD Biosciences).
Intracellular c-Maf, GATA-3, FoxP3, BCL6, Ki67 (Ab from BD Biosciences), and T-bet (eBioscience), staining was performed according to the manufacturer’s protocol (FoxP3 staining set protocol, eBioscience). Cells were analyzed by flow cytometry with a FACS Canto II (BD Biosciences) and analyzed with the FlowJo Software. Live cells were analyzed within a FSC-A/FSC-H gate to exclude cell doublets and triplets.

Andris F., Denanglaire S., Anciaux M., Hercor M., Hussein H, & Leo O. (2017). The Transcription Factor c-Maf Promotes the Differentiation of Follicular Helper T Cells. Frontiers in Immunology, 8, 480.

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T cell Phenotypic Characterization

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T cell restimulation and staining were as described in (21 (link)). Abs for staining were purchased from eBioscience (TCRβ, CD4, FoxP3, CD44, CD62L, c-Maf, Helios, RORγt, CD69, ICOS, CTLA-4, IL-10, IL-17A, IFNγ, Streptavidin); BD (CXCR5, TCRβ); Biolegend (PD-1); or R&D Systems (CCR2). All analyses are pre-gated on lymphocytes, singlets, live cells, and TCRβ⁺CD4⁺ cells unless otherwise stated.

Wheaton J.D., Yeh C.H, & Ciofani M. (2017). c-Maf is required for regulatory T cells to adopt RORγt+ and follicular phenotypes. Journal of immunology (Baltimore, Md. : 1950), 199(12), 3931-3936.

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