Huh7.5 cells and plasmid pFL-J6/JFH/JC1 containing the full-length chimeric HCV complementary DNA (cDNA) were provided by Vertex Pharmaceuticals, Inc. (Boston, MA, USA). HCV virus stock was prepared as previously described (18 (link)). Huh7.5 cells were infected with HCV virus stock (150 IU/cell) or virus mock stock (as a control). After 48 h, the culture supernatants were removed and the cells were lyzed directly with TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and total intracellular RNAs were extracted accordingly. mRNA microarray was performed by Kangchen Biotech Co., Ltd. (Shanghai, China) using Agilent chips (Agilent Technologies, Santa Clara, CA, USA). Each of the experiments was independently performed four times.
Agilent chips
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Sourced in China
Agilent chips are electronic components used in various laboratory and scientific instrumentation. They serve as integrated circuits that perform specific functions within the equipment. The core function of Agilent chips is to enable the precise control, measurement, and analysis of various samples and materials in a laboratory setting.
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2 protocols using agilent chips
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HCV infection and mRNA microarray
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Cerebral and Cerebellar Gene Expression in Mice
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Gene expression in cerebral cortex and cerebellum of mice were determined using Agilent chips. To ensure higher quality results in gene expression data, we conducted microarrays on 4 mice per group. Young mice were 2 months old and the other aged mice were 29 months old at the time of use. Data were standardized using global normalization and processed by R-program. An absolute fold change threshold of greater than 1.5 was required to be considered for further analyses. Expression values were in log2 scale. The original data analysed in this publication have been deposited in NCBI’s Gene Expression Omnibus and are accessible through GEO series accession number GSE75047.
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