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6 protocols using ultrapure water meter

1

Comprehensive Experimental Equipment Setup

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Thermo Fisher centrifuge (Thermo Fisher Science, USA); VORTEX-GENIE 2 vortex oscillator (SITM, USA); BX7200HP ultrasonic cleaner (Shanghai Xinmiao medical device manufacturing Co., Ltd.); AL104 balance with accuracy of 0.0001 (Mettler Toledo Shanghai Co., Ltd., Switzerland); CF16RN centrifuge (Hitachi, Japan); Ultrapure water meter (MilliPore, USA); and -80 °C ultra-low temperature refrigerator (Thermo Fisher science, USA).
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2

Instrument Protocols for Multidisciplinary Research

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The following instruments were used in this study: an ultra-clean workbench (Yida Purification Steel Structure Co., Ltd., Suzhou, China); low-temperature, high-speed centrifuge (Sigma Corporation, USA); super microplate reader (Bio-Tek Corporation, Winooski, VT, USA); inverted microscope (Nikon Corporation, Tokyo, Japan); carbon dioxide incubator (Sanyo Corporation, Osaka, Japan); ultrapure water meter (Millipore Corporation, Burlington, MA, USA); CL-32L-type High-Pressure Steam Sterilizer (ALP Corporation, Nagoya, Japan); vacuum pump (Anting Scientific Instrument Factory, Shanghai, China); constant temperature water bath (Yuhua Instrument Manufacturing Corporation, Zhengzhou, China); multi-purpose constant temperature ultrasonic extraction machine (Tian Xiang Instrument Co., Ltd., Huaian, China). In addition, high-performance liquid chromatography (HPLC, Agilent Technologies Inc., Santa Clara, CA, USA); centrifuge (Anting Scientific Instrument Factory, Shanghai, China); AB-204-N-type electronic balance (Mettler-Toledo Corporation, Shanghai, China); RE-52-type rotary evaporator (Ya Rong Biochemical Instrument Factory, Shanghai, China); and DZF-6050 vacuum drying oven (Qixin Scientific Instrument Co., Ltd., Shanghai, China) were also used.
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3

Fluorescence Microscopy and qPCR Analysis

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The fluorescent inverted microscope was from Olympus. The CO2 incubator was from Thermo Fisher Scientific, Inc. The fluorescence quantitative PCR instrument was from Applied Biosystems; Thermo Fisher Scientific, Inc. The ultrapure water meter was from Millipore, France. The high-speed low-temperature centrifuge was from Beckman Coulter, Inc.
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4

Detailed Cultivation and Identification of H. pylori Strains

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Sydney strain 1, H. pylori SS1 (VacA+, CagA-), and Hp49503 (VacA+, CagA+) strains were purchased from the Department of Pathogenic Biology, School of Medicine, Shanghai Jiao Tong University. Brucella Broth was provided by the Municipal Center for Disease Control and Prevention of Shanghai. Agar powder was purchased from Oxoid (UK). Simple culture tank with ventilation was prepared by the Shanghai Academy of Life Sciences, Chinese Academy of Sciences. Others included Gram staining kit (Shanghai Kang'en Biotech Co. Ltd), H. pylori detection kit (urease method) (Zhuhai Lituo Biotech Co. Ltd), diaminobenzidine hydrochloride (DAB) (Sigma, USA), mixed gas (85% N2, 10% CO2, 5% O2) (Shanghai Youjiali Liquid Nitrogen Co. Ltd.), high-pressure steam sterilizer (SANYO, Japan), ultraclean workbench (Suzhou Yongjiu Pressure Regulator Factory), CX31 microscope (Olympus, Japan), electronic balance (Sartorius, Germany), RM2145 slicer (Leica, Germany), ultralow temperature refrigerator (SANYO, Japan), CD177 antibody (ab8092; Abcam), UV inhibitor (Roche Diagnostics), UV UNIV MULT HRP (Roche Diagnostics), carbolic acid red dye solution (Beijing Leigen Biotech Co. Ltd), ultrapure water meter (Millipore, USA), electric thermostatic water bath (Shanghai Yiheng Co. Ltd), and benchtop horizontal centrifuge (Eppendorf, Germany).
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5

Ultrahigh-Performance Liquid Chromatography for Metabolite Analysis

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UHPLC/Q-Orbitrap system: Thermo Fisher Scientific’s Ultra 3000 UHPLC (USA), Thermo Fisher Scientific’s Q Exactive high-resolution mass spectrometer (USA). BX7200HP desktop ultrasonic cleaner from Shanghai Xinmiao Medical Equipment Manufacturing Co., Ltd.; AL104 balance (with an accuracy of 0.0001) from Mettler Toledo Shanghai Co., Ltd., Switzerland; VORTEX-GENIE 2 vortex oscillator from SITM (USA); Hitachi’s CF16RN Centrifuge (Japan); Heraeus Fresco 17 high-speed refrigerated centrifuge from Thermo Fisher Scientific (USA); Ultrapure water meter from MilliPore (USA).
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6

Rat Biomarker Quantification Protocol

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The ELISA kits for rat MTL, GAS, VIP, and ghrelin were purchased from Nanjing Jiancheng Technology Co., Ltd. (Nanjing, China). The other reagents were domestic and of analytical grade.
An ultrapure water meter (Millipore, USA), an Al104 electronic analytical balance (Shanghai METTLER TOLEDO Instrument Co., Ltd.), a Yp10002 Matou electronic balance (Shanghai Guangzheng Medical Instrument Co., Ltd.), a Tgl-5-a high-speed centrifuge (Shanghai Anting Analytical Instrument Co., Ltd.), a Varioskan flash full wavelength multifunctional microplate reader (Thermo, USA), a tissue homogenizer (Kinematica, Switzerland), and a 6890n gas chromatograph (Agilent, USA) were used.
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