Anti scgb1a1

Manufactured by Santa Cruz Biotechnology

Anti-Scgb1a1 is a laboratory product used to detect and study the Scgb1a1 protein in various biological samples. Scgb1a1 is a member of the secretoglobin protein family and is known to play a role in diverse physiological processes. Anti-Scgb1a1 can be utilized in techniques such as Western blotting, immunohistochemistry, and ELISA to facilitate research into the expression, localization, and function of Scgb1a1.

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Lab products found in correlation

Anti phospho erk1 2, by Cell Signaling Technology (1 mentions) Anti nkx2, by Santa Cruz Biotechnology (1 mentions) Anti ki67, by Abcam (1 mentions) Vectashield mounting medium containing dapi, by Vector Laboratories (1 mentions) Sm22α, by Abcam (1 mentions) Anti aqp5, by Abcam (1 mentions) Anti phospho histone3, by Cell Signaling Technology (1 mentions) Anti hdac3, by Santa Cruz Biotechnology (1 mentions) Anti sftpc, by Merck Group (1 mentions) Anti brdu, by Abcam (1 mentions) Anti acetylated tubulin, by Merck Group (1 mentions) Anti brdu, by Merck Group (1 mentions) T7451, by Merck Group (1 mentions) Anti krt5, by Fortrea (1 mentions)

Spelling variants of this product

Scgb1a1 (9 citations) Goat anti-Scgb1a1 (5 citations)

3 protocols using anti scgb1a1

Quantifying Lung Cell Markers

Cited 1 time

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Lung sections were processed and immunostained as described previously [31 (link)]. Sections were probed with anti-Scgb1a1 (Santa Cruz Biotech, CA), anti-Muc5ac (Millipore, CA), and anti-phospho ERK1/2 (Cell Signaling, CA) and the immunopositive cell numbers as well as the mean fluorescence intensity (MFI) were quantified using the NIH ImageJ software.

Chand H.S., Mebratu Y.A., Montera M, & Tesfaigzi Y. (2016). T cells suppress memory-dependent rapid mucous cell metaplasia in mouse airways. Respiratory Research, 17, 132.

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Histological Analysis of Lung Development

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Embryos were dissected, fixed in 4% paraformaldehyde overnight at 4°C, dehydrated through increasing gradient of ethanol washes, and embedded in the paraffin wax for tissue sectioning. Hematoxylin and Eosin (H&E) staining was performed using standard procedures. Immunohistochemistry was performed using the following antibodies: anti-HDAC3 (Santa Cruz, 1:10), anti-Aqp5 (abcam,1:100), T1-alpha(HybridomaBank,1:50), anti-Scgb1a1 (Santa Cruz, 1:20), anti-Sftpc (Millipore, 1:50), β-tublinIV (BioGenex1:20), SM22α (Abcam,1:100), anti-Sox2 (Seven Hills Bioreagents, 1:500), anti-phosphohistone 3 (Cell Signaling Technology, 1:200), anti-Nkx2-1 (Santa Cruz,1:50), anti-BrdU (Abcam,1:100), and anti-Ki67 (Abcam,1:50). Slides were mounted with Vectashield mounting medium containing DAPI (VectorLaboratories, Burlingame, CA, USA). For BrdU staining, pregnant mice were administrated intraperitoneally at 0.1mg/g body weight, 2 hours prior to harvest. Embryos were processed and sectioned as above.

Wang X., Wang Y., Snitow M.E., Stewart K.M., Li S., Lu M, & Morrisey E.E. (2016). Expression of histone deacetylase 3 instructs alveolar type I cell differentiation by regulating a Wnt signaling niche in the lung. Developmental biology, 414(2), 161-169.

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Immunofluorescence Staining of Cryosections

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8- to 10-μm cryosections were stained with: anti-acetylated tubulin (mouse, 1:1,000; Sigma, T7451), anti-BrdU (mouse, 1:500; Sigma, B8434), anti-Ecad (rat, 1:3000; Life Technologies, 13-1900), anti-Krt5 (rabbit, 1:500; Covance, PRB-160P), anti-T1α (1:1,000; DSHB, 8.1.1), and anti-Scgb1a1 (rabbit, 1:500; Santa Cruz, sc25555). Antigen retrieval was used for BrdU (2 N HCl 30 min 37°C, 0.5% trypsin 5 min, room temperature).

Watson J.K., Rulands S., Wilkinson A.C., Wuidart A., Ousset M., Van Keymeulen A., Göttgens B., Blanpain C., Simons B.D, & Rawlins E.L. (2015). Clonal Dynamics Reveal Two Distinct Populations of Basal Cells in Slow-Turnover Airway Epithelium. Cell Reports, 12(1), 90-101.

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