HDFs were plated into 96-well plates (5 × 103 per well) and incubated in growth medium for 24 h. After 16-h starvation with serum-free DMEM, HDFs were washed with PBS and irradiated with UVB (280–360 nm, peak at 313 nm) generated by a UV lamp (UVB-313EL, Q-Lab, USA) at 70 mJ/cm2 (duration: around 6 min). The irradiation dose was controlled by a digital UV meter (UV-340A, Lutron, Taiwan) whose detection wavelength ranges from 290 nm to 390 nm. Then, the cells were cultured with hucMSC-Exos at different concentrations (0, 10 and 30 μg/mL) in serum-free DMEM for 48 h. The control group was not exposed to UV irradiation and treated with serum-free DMEM. Afterwards, CCK-8 solution (10 µL; TransGen, China) was added into each well and incubated for 4 h. The absorbance of each well was measured at 450 nm using a Spark 10M microplate reader (Tecan, Switzerland).
Cck 8 solution
Manufactured by Transgene
Sourced in China
CCK-8 solution is a colorimetric cell viability assay kit. It is used to quantify the number of viable cells in proliferation, cytotoxicity, and cell growth studies.
Automatically generated - may contain errors
Lab products found in correlation
Model 680 microplate reader, by Bio-Rad (2 mentions)
Microplate reader, by Thermo Fisher Scientific (2 mentions)
Spark 10m microplate reader, by Tecan (1 mentions)
Uv 340a, by Lutron (1 mentions)
Synergy ht multi mode microplate reader, by Synergy Software (1 mentions)
Microplate reader, by Tecan (1 mentions)
Microplate reader, by Dynex (1 mentions)
Enspire, by PerkinElmer (1 mentions)
Microplate reader, by Molecular Devices (1 mentions)
Ldh activity assay kit, by Nanjing Jiancheng (1 mentions)
Image pro plus, by Nikon (1 mentions)
15 protocols using cck 8 solution
1
UVB-induced HDF Damage and hucMSC-Exos Protection
2
CCK-8 cytotoxicity assay for gastric cancer cells
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Cells (100 µl) were seeded into 96-well plates at concentrations of 10,000 and 8,000 cells/well for AGS and HGC-27, respectively. After 24 h, the gastric cancer cells were treated with different concentrations of Sal-B as described for the SRB assay for 48 h at 37°C. After treatment, 10 µl CCK-8 solution (TransGen Biotech Co., Ltd.) was added to each well, according to the manufacturer's protocol, and the cells were incubated at 37°C for 2 h. Subsequently, optical density values were measured at 450 nm using the Synergy HT Multi-Mode Microplate Reader. Drug-treated wells were compared with solvent-controlled wells using results obtained from three independent experiments.
3
Cell Viability Assay with CCK-8
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The cell suspension was evenly tiled onto 96-well cell plates (100 μL/well) and cultured in an incubator at 37 °C and 5% CO2 for 24–72 h to make the cells adherent to the wall. Then, 100 μL of drugs of different concentrations (0 ng/mL, 1 ng/mL, 5 ng/mL, 10 ng/mL and 20 ng/mL) were added to each well cell, and each group was cultured for 72 h. Before adding CCK-8, we replaced fresh culture medium to remove the influence of the drug, added 10 μL of CCK-8 solution (Transgen BioTECH, China) to each well, and incubated in the incubator in the dark for 1 h. The absorbance OD value was measured at a wavelength of 450 nm.
4
Cell Proliferation Assay with CCK-8
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Cells were seeded into 96-well plates at a density of 1000 cells in 100 μL of complete medium per well. At each time point, the original medium was replaced with a 1:9 mixture of CCK-8 solution (Transgene) and complete medium, and the cells were then incubated at 37°C for 2 h. The absorbance of each sample at 450 nm was analyzed by a microplate reader (Tecan), and each sample was measured three times.
5
Cell Viability Assay of Transfected HT22 Cells
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The transfected HT22 cells (5×103/well) were seeded into 96-well plates and maintained at 37 ℃ overnight. The cells were cultivated for another 2 h at 37 ℃ after the addition of 10 µL of CCK-8 solution (Beijing Transgen Biotech Co., Ltd.). With the aid of a microplate reader (Dynex Technologies), the absorbance was read at 450 nm.
6
Gastric Cancer Cell Viability Assay
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Gastric cancer cells were seeded into 96‐well plates and transfected with different genes for 48 hours. Cells were then subjected to a cell viability assay, comprising up to 120‐hour incubation. At the end of each experiment, the cell culture was combined with 10 μl of CCK‐8 solution (TransGen Biotech), further incubated for 4 hours, and the optical density value was measured by using a microplate reader (Thermo Scientific) at the absorbance at 450 nm. The experiments were performed in triplicate and repeated at least three times.
7
Cytotoxicity Evaluation of Oleanolic Acid
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The cytotoxicity of OA in cells was assayed using the CCK-8 staining method [60 (link)]. In brief, HepG2 or L02 cells (5 × 103 cells per well) were seeded in 96-well plates overnight at 37 °C and 5% CO2. The medium was removed, and serial twofold dilutions of OA or 10% BSA were applied for 1, 2, and 5 days. After incubation on the corresponding days, 10 µL of CCK-8 solution (#FC101-04, TransGen, Beijing, China) was added to each well and incubated at 37 °C for 2 h. The absorbance was measured at 450 nm using Enspire (Perkin Elmer, Waltham, MA, USA).
8
Cell Proliferation Assay with CCK-8
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HCC cells suspensions were added to a 96-well plate at a density of 1000–2000 cells in 100 μl of complete medium and cultured overnight. A total of 10 μl CCK-8 solution (TransGen Biotech Ltd., Beijing, China) was added to each well at different time points (0h, 24h, 48h, 72h, 96h). The absorbance at 450 nm was measured using a microplate reader (Molecular Devices, USA) after 2 h incubation.
9
CCK-8 Assay for EVs-mediated Chondrocyte Protection
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CCK-8 was used to detect the effect of EVs on IL-1β-induced-chondrocyte injury. Chondrocyte cells (2 × 103) were cultured in a 96-well plate overnight, and co-cultured with different EVs concentrations (0, 1, 5, 10, 25, and 50 μg/ml) for 24 h. A control group was only co-cultured with normal culture media. According to the manufacturer’s protocol, cells were incubated with 10 μl of CCK-8 solution (TransGen, Beijing, China) in each well for 4 h at 37°C. Finally, the optical density (OD) value at 450 nm of each well was measured by using a microplate reader (Perlong, Beijing, China).
10
Cell Proliferation Assay with CCK-8
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The Cell Counting Kit-8 (CCK-8) assay was used to assay cell proliferation. Briefly, the SFFs and HaCaT cells were seeded in a 96-well plate (2 × 104 cells/well) and cultured in the DMEM-F12 and DMEM medium, respectively, supplemented with 10% FBS and 1% penicillin/streptomycin. The cells were individually transfected with pCMV-Myc, pCMV-Myc-FST or pCMV-Myc-STAT3 for 24, 48, and 72 h, every well was added with 10 μL CCK-8 solution (TransGen, China) and incubated at 37°C for 2 h, following the absorbance was measured at 450 nm using a Model 680 Microplate Reader (Bio-Rad, United States). The cells transfected with pCMV-Myc were used as the negative control.
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