Vβ8 1 8

Manufactured by BD

The Vβ8.1/8.2 is a laboratory equipment product designed for research purposes. It is used to detect and analyze specific T cell receptor subunits. The device functions by identifying the presence and expression levels of the Vβ8.1 and Vβ8.2 T cell receptor variable regions.

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Lab products found in correlation

Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Purified rat anti mouse cd16 cd32, by BD (1 mentions) Cd103, by BD (1 mentions) Tnfα mab11, by BioLegend (1 mentions) Anti eomes dan11mag, by Thermo Fisher Scientific (1 mentions) Batimastat bb 94, by Abcam (1 mentions) Facsc, by BD (1 mentions) Fluorochrome conjugated antibodies, by BioLegend (1 mentions) Dulbecco s pbs, by Thermo Fisher Scientific (1 mentions) Anti cd16 cd32, by BD (1 mentions) Facscalibur, by BD (1 mentions) Cellquest pro software, by BD (1 mentions)

3 protocols using vβ8 1 8

Fluorophore-conjugated antibodies for flow cytometry

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Fluorophore-conjugated murine antibodies were purchased from eBioscience, including antibodies specific for CD8a (53–6.7; catalog #48-0081-80), Thy1.1 (HIS 51; 25–0900-82), Tim3 (RMT3-23; 14–5870-81), Lag3 (ebioC9B7W; 12–2231-81), CD137 (17B5; 17-1370-80), KLRG1 (2F1; 175893–81), and CD25 (PC 61.5; 17–0251-81). Other antibodies to FasL (K10; 106805), vβ9 (MR10-2; 553201), CD69 (H1.2F3; 104502), CD127 (A7R34; 135013), CD80 (16-10A1; 553768), CD86 (GL-1; 105011), IL-2 (JES6-5H4; 503807), IFNγ (XMG1.2; 505809), TNFα (Mab11; 502913), Bax (6A7; 633801), and Bcl-2 (10C4; 633507) were purchased from Biolegend. 7AAD (A1310) and other antibodies were purchased from BD Biosciences, including Fas (Jo2; 563647), Thy1.2 (53–2.1; 561616), CD103 (M290; 557495), purified rat anti-mouse CD16/CD32 (2.4G2; 12–4875-80), PD-1 (J43; 11–9985-81), and Vβ8.1/8.2 (553185). Antibodies against pAKT (S473; D9E; 5315S) were purchased from Cell Signaling Technology. Anti-Eomes (DAN11 mag; 12–4875-80) and the LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (L34957) were purchased from ThermoFisher. For FasL detection, Panc1 cells were treated for 24 h with 3 µg/ml Batimastat (BB-94; ab146619) purchased from Abcam. Flow cytometry data were acquired on a BD FACS Canto II instrument or LSR II and analyzed with Flowjo v9 software.

Oda S.K., Anderson K.G., Ravikumar P., Bonson P., Garcia N.M., Jenkins C.M., Zhuang S., Daman A.W., Chiu E.Y., Bates B.M, & Greenberg P.D. (2020). A Fas-4-1BB fusion protein converts a death to a pro-survival signal and enhances T cell therapy. The Journal of Experimental Medicine, 217(12), e20191166.

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Tetramers for Immune Cell Analysis

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Mouse and human CD1d tetramer (TET⁺) unloaded or loaded with α-galactosylceramide (α-GalCer) analog PBS57 and human MR1 tetramer (TET⁺) loaded with 6-FP (control) or 5-OP-RU were provided by NIH tetramer facility. Fluorochrome-conjugated antibodies against mouse CD3, CD4, CD8α, CD69, NK1.1, TCRβ, Ly6G, CD11b, B220, CD11c, Ly6C, F4-80, IFN-γ, IL-17A, Vβ2, Vβ7, and Vβ12 and human CD3, CD4, CD8α, Vα7.2, CD161, CD14, and CD19 were purchased from BioLegend. Vβ4, Vβ5.1/5.2, Vβ6, Vβ8.1/8.2, Vβ9, Vβ11, and Vβ13 were purchased from BD. Anti-Vβ10 was purchased from eBioscience. Live dead eFlour506 was purchased from Thermo Fischer Scientific.

Genardi S., Visvabharathy L., Cao L., Morgun E., Cui Y., Qi C., Chen Y.H., Gapin L., Berdyshev E, & Wang C.R. (2020). Type II Natural Killer T Cells Contribute to Protection Against Systemic Methicillin-Resistant Staphylococcus aureus Infection. Frontiers in Immunology, 11, 610010.

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Multicolor Flow Cytometry Analysis

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Muscle leukocyte suspensions were incubated with anti-CD16/CD32 (anti-mouse, clone 2.4G2; BD) to block binding to Fc receptors before staining. The following mouse mAbs used for staining were all obtained from BD: CD3e (hamster anti-mouse, clone 145-2C11), Vβ8.1/8.2 (mouse anti-mouse, clone MR5-2), NK1.1 (mouse anti-mouse, clone PK136), and pan-NK (rat anti-mouse, clone DX5). Antibodies were conjugated to FITC, PE, and/or Cy5. The following antibodies were biotinylated and revealed with Tricolor-conjugated streptavidin (Ebioscience): anti–asialo-GM1 (CL8955; Cedarlane Laboratories) or rabbit sera (control; Invitrogen). Optimal working dilutions were determined for each antibody before use. All incubations were performed in Ca⁺,Mg⁺-free Dulbecco’s PBS (Invitrogen) at 4°C for 30 min. After the last wash, 10³ live cells per sample were acquired on a FACScalibur (Becton Dickinson) and analyzed with CELL Quest Pro software (Becton Dickinson). CD1d tetramers were obtained from the National Institutes of Health Tetramer Core Facility.

Capote J., Kramerova I., Martinez L., Vetrone S., Barton E.R., Sweeney H.L., Miceli M.C, & Spencer M.J. (2016). Osteopontin ablation ameliorates muscular dystrophy by shifting macrophages to a pro-regenerative phenotype. The Journal of Cell Biology, 213(2), 275-288.

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