Solvents used were puriss, with the exception of THF, which was purified by fresh distillation over Na/benzophenone. Commercially available reagents were used as received, without any further purification. Nilotinib was purchased from Selleck Chemicals, Munich, Germany. Analytical TLC was performed on commercial Merck silica gel 60 F254. Flash chromatography was carried out using silica gel 60 (230–400 mesh). HPLC experiments were performed using a Shimadzu system, consisting of a DGU-20A controller, an LC-20AD pump, an SPD-M20A photodiode-array detector and a CTO-10AS column oven. 1H and 13C NMR spectra were recorded on a Brucker AMX (400/100 MHz 1H/13C) spectrometer using tetramethyl silane (TMS) as an internal standard. Chemical shifts are reported in ppm (δ) referenced to TMS, coupling constants J in Hz. High-resolution ESI mass spectra were measured on a Thermo Fisher Scientific LTQ ORBITRAP/LC−MS system. Elemental analyses were performed on a Heraeus CHN-Rapid Analyser.
Ltq orbitrap lc ms system
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
The LTQ ORBITRAP/LC−MS system is a high-performance mass spectrometry platform for chemical analysis. It combines the LTQ linear ion trap mass spectrometer with the Orbitrap mass analyzer, providing high mass accuracy and resolution for the detection and identification of compounds in complex samples. The system is designed for applications that require precise mass measurements, such as metabolomics, proteomics, and small molecule analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ltq orbitrap lc ms system
1
Purification and Characterization of Nilotinib
2
High-Resolution ESI-LC-MS Metabolomics Workflow
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HR-ESI-LC-MS was obtained using an LTQ Orbitrap LC-MS system (Thermo Scientific, UK) coupled to a Thermo Instrument HPLC system (Accela PDA detector, Accela PDA autosampler and Accela Pump) on a positive ESI mode (30,000), MS/MS resolution 7500, C18 (Sunfire 150 × 46 mm column). 0.1% formic acid in water and 0.1% formic acid in acetonitrile was used for reverse-phase separation using a gradient from 0–100% in 25 min. The instrument parameters were set as following: Capillary voltage 45 V, spray voltage 4.5 kV, capillary temperature 200 °C, auxiliary gas flow rate 10–20 arbitrary units, sheath gas flow rate 5 arbitrary units, mass range 150–2000 amu (maximum resolution 30,000×), MS scan 150–2000Da coupled with an automated full dependent MS-MS scan.
1D and 2D NMR data were obtained on a Bruker AVANCE III HD 600 MHz (AscendTM14.1 Tesla, UK) with Prodigy TCITM cryoprobe at 298 K in DMSO-d6 and CD3OD (Goss Scientific). Trimethylsilane (TMS) was used as an internal standard.
1D and 2D NMR data were obtained on a Bruker AVANCE III HD 600 MHz (AscendTM14.1 Tesla, UK) with Prodigy TCITM cryoprobe at 298 K in DMSO-d6 and CD3OD (Goss Scientific). Trimethylsilane (TMS) was used as an internal standard.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!