IPGTT was performed after 12 weeks of instillation. Brie y, after a 16-hour fasting, the basal blood glucose levels of mice were determined using an automatic glucometer (Glucotrend 2, Roche Diagnostics). The mice were then intraperitoneally injected with glucose (2 g/kg of body weight) and the blood glucose levels at 15, 30, 60, and 120 min after injection of glucose were measured as described above. ITT was performed after 13 weeks of instillation. Brie y, after a 4-hour fasting, the basal blood glucose levels of mice were determined using an automatic glucometer (Glucotrend 2, Roche Diagnostics). The mice were then intraperitoneally injected with insulin (0.5 U/kg of body weight), and the blood glucose levels at 15, 30, 60, and 120 min after injection of insulin were measured as described above.
Glucotrend 2
Manufactured by Roche
Sourced in Germany
The Glucotrend 2 is a compact handheld blood glucose monitoring device designed for personal use. It provides quick and reliable measurements of blood glucose levels, allowing users to effectively manage their diabetes or monitor their overall health.
Automatically generated - may contain errors
Lab products found in correlation
Streptozotocin (stz), by Merck Group (2 mentions)
Sodium citrate tribasic dehydrate, by Merck Group (1 mentions)
Au400 chemistry analyzer, by Olympus (1 mentions)
Stz solution, by Merck Group (1 mentions)
Elisa, by DRG International (1 mentions)
Ultra sensitive mouse insulin elisa kit, by Crystal Chem (1 mentions)
16 protocols using glucotrend 2
1
Glucose and Insulin Tolerance Tests
2
Glucose Tolerance Test in Mice
3
Intraperitoneal Glucose Tolerance Test
4
Insulin Sensitivity Test in Mice
5
Streptozotocin-Induced Diabetic Rat Model
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Diabetes was induced by an intraperitoneal (i.p.) injection of 55 mg/kg of streptozotocin (STZ; Sigma-Aldrich, St. Louis, MO, USA). Controls were given (vehicle) 50 mM trisodium-citrate buffer (sodium citrate tribasic dehydrate; Sigma-Aldrich), pH 4.5. After STZ injection, the animals were placed individually in metabolic cages, and diabetes was confirmed 16 h later if pronounced glucosuria and polyuria had developed. Before the animals were sacrificed, their fasting blood glucose was measured using a glucometer (Glucotrend 2; Roche, Mannheim, Germany) in blood samples taken from the tail. In a group of 6 DI rats, the blood glucose was measured every 12 h until the day of sacrifice.
6
Glucose Tolerance Test in Aged Mice
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After 46-week-exposure to FA/CAP, mice were subjected to IPGTT on that Sunday. Before testing, mice (50 weeks old) were fasted (initiated immediately after the Saturday’s exposure) for 16 h. On the day of experiments, the basal glucose level of tail vein blood was determined using an automatic glucometer (Glucotrend 2, Roche Diagnostics), and then mice were intraperitoneally injected with glucose (2 g/kg body weight). The glucose levels of the tail vein blood at 15, 30, 60, and 120 min after injection was measured as described above.
7
Insulin Tolerance Test in Mice
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ITT was performed on mice after the 47-week-exposure to FA/CAP on that Sunday. Before testing, mice (51 weeks old) were fasted for 4 h. The basal glucose level of tail vein blood was determined using an automatic glucometer (Glucotrend 2, Roche Diagnostics) and then mice were intraperitoneally injected with insulin (0.5 U/kg body weight). The glucose levels of the tail vein blood at 15, 30, 60, and 120 min after injection was measured as described above.
8
Glucose and Insulin Tolerance Tests
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For GTTs, randomized male mice (n=6) were fasted for 16 h and then injected intraperitoneally with 2 g kg−1 body weight glucose. Blood glucose values were determined by an automatic glucose monitor (Glucotrend 2, Roche) and Accu-Chek active bands (Roche)56 (link). The ITT was performed on mice fasted for 4 h, human insulin was administered by IP injection at 1 U kg−1. Blood glucose levels were monitored as above immediately before injection (T0), and at 30, 60 and 120 min after injection. Blood was collected by retro-orbital puncture, centrifuged at 5,000g for 10 min and plasmas were used for the measurement of fasting blood glucose, cholesterol, triglycerides, non-essential fatty acids levels and markers of hepatic function by an Olympus AU400 chemistry analyzer.
9
Streptozotocin-Induced Diabetic Wound Healing
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Twenty SD rats were used in this experiment. A single dose of 62 mg/kg streptozotocin (STZ) (Sigma‐Aldrich, St. Louis, MO) was injected intravenously to induce diabetes.15 Blood glucose levels were tested with a blood glucose monitoring system (Glucotrend 2, Roche Diagnostics, Mannheim, Germany). A diabetic state was diagnosed when polydipsia, polyphagia, polyuria, weight loss, and hyperglycaemia (16.7 mmol/L) were noted. Rats were hyperglycaemic for 8 weeks before surgery. Full‐thickness wounds (1.0 cm diameter) were made on the back of each rat with a sterilised punch equidistant from the midline. Tissues that were cut with the punch were used as the diabetic control group. Wound tissues and the surrounding areas were harvested 2 and 4 weeks after injury.
10
Glucose Tolerance and Insulin Sensitivity Evaluation
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An intraperitoneal glucose tolerance test (ipGTT) was performed in each group by intraperitoneal injection of a 20% glucose solution at a dose of 2 g/kg. Tail vein blood glucose levels were measured using a Roche blood glucose monitor (Glucotrend 2, Roche, Germany) in samples taken immediately before the glucose injection and at 30, 60, and 120 minutes after. Fasting insulin levels were quantified using a commercially available radioimmunoassay kit (China Institute of Atomic Energy, Beijing, China). Insulin sensitivity and β-cell capability of individual animals was evaluated using the homeostasis model assessment (HOMA) index [19 (link)]. The formula used was the following:
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