Anti prdm16

The Western Blot analysis was conducted as previously described (Li et al., 2015, 2009), primary antibodies: anti‐UCP1 was purchased from Cell Signalling Technology (#14670), anti‐PRDM16 was purchased from Abcam (#ab202344), anti‐β‐actin was purchased from Proteintech (#HRP‐60008). All validation information could be found on the manufacturer's website.

Anti-β-actin (Millipore, Temecula, CA; MAB1501), anti-UCP1 (Abcam, Cambridge, MA, USA; ab10983), anti-phospho (T172) AMPKα (Abcam, Cambridge, MA, USA; ab2535), anti-AMPKα (Abcam, Cambridge, MA, USA; ab2532S), anti-Prdm16 (Abcam, Cambridge, MA, USA; ab106410), goat anti-mouse immunoglobulin G (IgG) (Sigma, St. Louis, MO, USA; #AP124P), goat anti-rabbit (Sigma, St. Louis, MO, USA; #401353), anti-4,6-diamidino-2-phenylindole (Sigma, St. Louis, MO, USA), and anti-rabbit antibody Alexa Fluor 594 (Invitrogen, Carlsbad, CA, USA; A11012) were used in this study. Rg3 was purchased from Abcam (Abcam, Cambridge, MA, USA; ab141938).

Chromatin immunoprecipitation (ChIP), immunoblotting, immunofluorescence, or immunohistochemistry were performed using the following antibodies: anti-α-SMA (#19245T; Cell Signaling); anti-β-Actin (#64225332; Bio-Rad), anti-amylase (#ab21156; Abcam), anti-chromogranin-A (#ab45179; Abcam), anti-cytokeratin 19 (#ab52625; Abcam), anti-E-cadherin (#3195S; Cell Signaling), anti-glucagon (#2760; Cell Signaling), anti-insulin (#4590; Cell Signaling), anti-JunB (#3753; Cell Signaling), anti-Muc5AC (#ab3649; Abcam), anti-Prdm16 (#ab202344 and #ab106410; Abcam), anti-Smad2 (#5339; Cell Signaling), anti-Smad3, (#9523; Cell Signaling), anti-Smad4, (#46535; Cell Signaling), anti-Smad4 (#sc-7966; Santa Cruz), anti-Smad2/3 (#8685; Cell Signaling), and anti-vimentin (#5741S; Cell Signaling).