L ascorbic acid

Single-mode optical fibers (SMF-28e) were purchased from Corning Optical Communications (Berlin, Germany). Ethanol, glycerol, (3-glycidyloxypropyl)trimethoxysilane (GOPTS), L-cysteine, phosphate buffered saline (PBS; pH = 7.4), H₂O₂ (ω = 30%), potassium hydroxide, and urea were purchased from Sigma-Aldrich (Taufkirchen, Germany). Acetic acid, CaCl₂·2H₂O, 1 M HCl, MgCl₂·6H₂O, L-ascorbic acid, KCl, NaCl, 1 M NaOH, H₂SO₄ (ω = 96%) and Tris base were purchased from Carl-Roth (Karlsruhe, Germany). Hydrofluoric acid (HF; ω = 40%) and immersion oil were purchased from AppliChem (Darmstadt, Germany). Water was purified with a Milli-Q purification system. Human recombinant C-reactive protein (CRP; β = 1 mg/mL) was purchased from BioCat (Heidelberg, Germany) and pooled human >97% CRP deficient plasma was purchased from Dunn Labortechnik (Asbach, Germany). The CRP-specific single-stranded DNA aptamer (CRP-40-17-3′SH) with a thiol group at the 3′-end was synthesized and HPLC was purified by Metabion (Planegg, Germany) and delivered at 100 µM in bidest. water. The sequence of CRP-40-17-3′SH was: 5′-CCC CCG CGG GTC GGC TTG CCG TTC CGT TCG GCG CTT CCC CTT TTT TTT T-C6-SH-3′ [50 (link)].

4-Cyanobenzoic acid (98%, abcr), 4-(dimethylamino)pyridine (99%, Aldrich), deuterium oxide (99.9 atom % D, Aldrich), hydrochloric acid (37%, lab reagent grade, Fisher Scientific), L-ascorbic acid (≥99%, Roth), N,N’-dicyclohexylcarbodiimide (99%, Aldrich), poly(ethylene glycol) methyl ether (M_n = 4830 g·mol⁻¹, Aldrich), silver nitrate (≥99%, Sigma-Aldrich), sodium hydroxide (>99%, Roth), and trisodium citrate dihydrate (99.7%, Merck) were used as received. Water with a resistivity of 18.2 MΩ·cm (Milli-Q) was used for particle synthesis and purification.

All utilized chemicals were obtained commercially and used without further purification. Tetrachloroauric(III) acid trihydrate (HAuCl₄ ≥ 99.5%) and L(+)-ascorbic acid (AA, ≥99%) were purchased from Carl Roth GmbH & Co KG (Karlsruhe, Germany). Hexadecyltrimethylammonium chloride (CTAC, >99%) implemented in synthesis procedure was obtained from Molekula Group GmbH (Munich, Germany). Hexadecyltrimethylammonium chloride (CTAC, 25 wt% in water) implemented in purification procedure, sodium borohydride (NaBH₄, 99.99%), and sodium idode (NaI, ≥99.5%) were purchased from Sigma-Aldrich (Darmstadt, Germany). The solutions were prepared using Millipore water. Prior to use, all glassware and magnetic stirrers were washed with aqua regia (caution: aqua regia is highly toxic and corrosive) and rinsed thoroughly with Millipore water.

All chemicals were purchased
and used without further purification.
Propan-2-ol (C₃H₈O, ≥99.5%), ethanol
(EtOH, ≥99.8%), methanol (MeOH, ≥99.5%), acetone (C₃H₆O, ≥99.5%), and hydrochloric acid (HCl,
37.0%) were purchased from VWR. Nitric acid (HNO₃, ≥65%),
sodium hydroxide pellets (NaOH, ≥98.0%), sodium borohydride
(NaBH₄, ≥97%), and l(+)-ascorbic acid (AA,
≥99%,) were purchased from Carl Roth. Hydrogen tetrachloroaurate
trihydrate (HAuCl₄·3H₂O, ≥99.9%),
hexadecyltrimethylammonium bromide (CTAB ≥99%), cetyltrimethylammonium
chloride solution (CTAC, 25 wt % in H₂O), hexadecylpyridinium
chloride monohydrate (CPC, 99.0–102.0%), (3-aminopropyl)triethoxysilane, N,N-diisopropylethylamine (DIPEA), and
poly(sodium 4-styrenesulfonate, M_w of
70000 g/mol) were purchased from Sigma-Aldrich. 3-(Triethoxysilyl)propylsuccinic
anhydride, 3-acetoxypropyltrimethoxysilane, n-butyltriethoxysilane, and heptadecafluoro-1,1,2,2-tetrahydrodecyl)triethoxysilane
were purchased from abcr. (α-Thiol, ω-bromo)-terminated
poly(acrylic acid) (PAA-SH, M_w of 8500
g/mol) was purchased from Polymer Source Inc. Mica sheets were obtained
from Micro to Nano with different shapes and a thickness of 0.15 to
0.21 mm and the highest grade V-1 quality. Double-polished Si wafers
(orientation [100] of 5 mm length and 7 mm width) from Siegert Wafer
were used for ellipsometry measurements. Milli-Q water (18.2 MΩ·cm)
was used in all experiments.

The used Fe^II-triazole complexes and composite materials were synthesized by using the following purchased chemicals without further purifying them: Iron(II)Chloride tetrahydrate (FeCl₂ · 4 H₂O) (>99%) from Sigma-Aldrich (St. Louis, MO, USA); L-ascorbic acid (>99%) from Carl Roth; 4-Amino-1,2,4-Triazole (99%) purchased from Thermo Scientific (Waltham, MA, USA); Sodium 2-Naphthalenesulfonate (98%) from Alfa Aesar (Haverhill, MA, USA); PMMA 350,000 Mw from Sigma-Aldrich (St. Louis, MO, USA); and 2,2,2-Trifluorethanol (TFE) from Carl Roth. The measured Mössbauer spectra were recorded in transmission, and ⁵⁷Co/Rh source was used.
Two different complexes were used in the two approaches performed, with only the corresponding anions being changed. This change in anions should result in slight differences in the thermally induced SCO effect, but it is not expected to significantly affect other properties of the composite material.
The decision regarding the applied potential and concentration for the spinning parameters was previously made by our working group, as these values were found to yield the best results in this context.

Human iPSCs were generated from plucked human hair keratinocytes and from human foreskin fibroblasts (System Biosciences). Keratinocytes were cultured and infected as described in^{32 (link)}. Fibroblasts were cultured in DMEM, 10% FBS, 1% antibiotic-antimycotic, 1% NEAA, and 1% GlutaMAX. For reprogramming 1*10⁵ fibroblasts were plated on coated 6-well plates and were infected with 5*10⁸ viral copies of STEM CCA³⁹ OKSM lentivirus on two subsequent days in culture medium supplemented with 10 µM Rock inhibitor/Y-27632 (Selleckchem), 8 µg/ml polybrene (Sigma Aldrich). On the third day infected keratinocytes and fibroblasts were distributed equally into 6-well plates on mitomycin-inactivated rat embryonic fibroblast (REF) feeder cells. 1,5*10⁴ REFs were mitotically inactivated with 7,5 µg/ml mitomycin C for 2,5 h. During reprogramming cells were cultured in KO-DMEM, 20% KOSR, 1% antibiotic-antimycotic, 100 μM NEAA, 1% GlutaMAX, 50 mM β-mercaptoethanol, 50 μg/ml L-Ascorbic acid (Carl Roth), 10ng/ml FGF2 (Cell Guidance Systems), 10 µM Rock inhibitor/Y-27632 (Selleckchem) at 5% CO2, 5% O2, and 37 °C, and medium was changed every second day. IPSC colonies were mechanically transferred onto Matrigel coated (Corning) 6-well plates after three weeks.