Anti sf3b1

Manufactured by Fortis Life Sciences

Anti-SF3B1 is a laboratory reagent used in biological research. It functions as an inhibitor of the SF3B1 protein, which is involved in pre-mRNA splicing. The product can be used to study the role of SF3B1 in various cellular processes.

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Lab products found in correlation

Anti actin, by Merck Group (3 mentions) Anti sugp1, by Fortis Life Sciences (3 mentions) Goat anti mouse igg, by LI COR (2 mentions) Anti dykddddk, by GenScript (2 mentions) Donkey anti rabbit igg, by LI COR (2 mentions) Anti gst, by Thermo Fisher Scientific (2 mentions) Anti phf5a, by Proteintech (1 mentions) Flavopiridol, by Merck Group (1 mentions) Mitoxantrone, by Merck Group (1 mentions) Topotecan, by Merck Group (1 mentions) Camptothecin, by Selleck Chemicals (1 mentions) Doxorubicin, by Merck Group (1 mentions) B ap15, by Selleck Chemicals (1 mentions) Rabbit anti usp7, by Fortis Life Sciences (1 mentions) P5091, by Selleck Chemicals (1 mentions) Rabbit anti cleaved notch1, by Cell Signaling Technology (1 mentions) Ml323, by Merck Group (1 mentions) Bml277, by Merck Group (1 mentions) Sch900776, by MedChemExpress (1 mentions) Pr 619, by Merck Group (1 mentions)

4 protocols using anti sf3b1

Immunoblotting Analysis of SF3B1 in Transfected Cells

Cited 1 time

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For immunoblotting of SF3B1/FLAG-SF3B1proteins in transiently-transfected HEK293T and stably-transduced TF1 and K562 cells in
which transcriptome analysis was done in parallel, Western blotting using a mouse
anti-human-SF3B1 antibody (Abcam #172634) at 1:1000 dilution was used.
Immunoblotting following affi nity purification of SF3B1 in HEK293T and TF1 cells was
performed as previously described, and primary antibodies were: anti-SF3B1 (Bethyl
Laboratories, A300–996A, 1:1,000), anti-ACTIN (Sigma, A2066, 1:2,000),
anti-DYKDDDDK (GenScript, A00187, 1:1,000), anti-SUGP1 (Bethyl
Laboratories A304–675A-M, 1:1,000), and anti-PHF5A (Proteintech 15554–1-AP,
1:1000)^{17 (link)}. Secondary antibodies
were: Donkey anti-Rabbit IgG (LI-COR, 926–68073, 1:5,000) and Goat anti-Mouse IgG
(LI-COR, 926–32210, 1:5,000).

Choi I.Y., Ling J., Zhang J., Helmenstine E., Walter W., Bergman R., Philippe C., Manley J., Rouault-Pierre K., Li B., Wiseman D., Ouseph M., Bernard E., Li X., Haferlach T., Fazal S., Jain T., Gocke C., DeZern A, & Dalton W.B. (2023). The E592K variant of SF3B1 creates unique RNA missplicing and associates with high-risk MDS without ring sideroblasts. Research Square.

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Antibody-based Western Blotting and Immunoprecipitation

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The following antibodies were used for Western blotting or immunoprecipitation: anti-SF3B1 (Bethyl Laboratories, A300-996A-T, MBL International, D221-3), rabbit anti-cleaved Notch1 (Cell Signaling Technology, 4147S), rabbit anti–β-actin (Cell Signaling Technology, 4967S), rabbit anti-USP7 (Bethyl Laboratories, A300-033A), rabbit anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (Cell Signaling Technology, 2118S), rabbit anti-CHEK2 (Bethyl Laboratories, A300-618A), mouse immunoglobulin G2b (IgG2b) (MBL International, M077-3), P5091 (Selleck Chemicals, S7132), PR619 (Sigma-Aldrich, SML0430), b-AP15 (Selleck Chemicals, S4920), ML323 (Sigma-Aldrich, SML1177), BML277 (MedChem Express, HY-13946), BML277 (Sigma-Aldrich, C3742, in vivo), SCH900776 (MedChem Express, HY-15532), camptothecin (Selleck Chemicals, S1288), flavopiridol (Sigma-Aldrich, F3055), topotecan (Sigma-Aldrich, T2705), etoposide (Sigma-Aldrich, E1383), mitoxantrone (European pharmacopoeia reference standard, M2305000), doxorubicin (Sigma-Aldrich, 44583), and E7107 and H3B-8800 (H3 Biomedicine).

Han C., Khodadadi-Jamayran A., Lorch A.H., Jin Q., Serafin V., Zhu P., Politanska Y., Sun L., Gutierrez-Diaz B.T., Pryzhkova M.V., Abdala-Valencia H., Bartom E.T., Buldini B., Basso G., Velu S.E., Sarma K., Mattamana B.B., Cho B.K., Obeng R.C., Goo Y.A., Jordan P.W., Tsirigos A., Zhou Y, & Ntziachristos P. (2022). SF3B1 homeostasis is critical for survival and therapeutic response in T cell leukemia. Science Advances, 8(3), eabj8357.

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Western Blot Antibody Optimization

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Western blotting was performed as described (Zhang et al. 2019a (link)) with the following primary antibodies: anti-SF3B1 (1:1000; Bethyl Laboratories A300-996A), anti-GST (1:1500; Invitrogen A5800), anti-SUGP1 (1:1000; Bethyl Laboratories A304-675A-M), anti-DDX46 (1:1000; Bethyl Laboratories A301-052A-T) used with Immuno Shot reagent 1 (Cosmo Bio), anti-HA rabbit (1:1000; Abm G166), anti-HA mouse (1:1000; Sigma-Aldrich H3663), and anti-ACTIN (1:2000; Sigma-Aldrich A2066).

Zhang J., Xie J., Huang J., Liu X., Xu R., Tholen J., Galej W.P., Tong L., Manley J.L, & Liu Z. (2023). Characterization of the SF3B1–SUGP1 interface reveals how numerous cancer mutations cause mRNA missplicing. Genes & Development, 37(21-24), 968-983.

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Immunoblotting of Protein Complexes

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Proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad), followed by immunoblotting with primary and secondary antibodies. Primary antibodies were: anti-SF3B1 (Bethyl Laboratories, A300–996A, 1:1,000), anti-ACTIN (Sigma, A2066, 1:2,000), anti-HA rabbit polyclonal (Abm, G166, 1:1,000), anti-HA mouse monoclonal (Sigma, H3663, 1:1,000), anti-DYKDDDDK (GenScript, A00187, 1:1,000), anti-SUGP1 (Bethyl Laboratories, A304–675A-M, 1:1,000), anti-RBM6 (ABclonal, A10391, 1:1,000), anti-U2AF2 (Sigma, U4758, 1;10,000), and anti-GST (Invitrogen, A5800, 1:1,500). Secondary antibodies were: Donkey anti-Rabbit IgG (LI-COR, 926–68073, 1:5,000), and Goat anti-Mouse IgG (LI-COR, 926–32210, 1:5,000). Immunofluorescence was detected using either the Odyssey Infrared Imager (LI-COR) or the ChemiDoc Imaging System (Bio-Rad).

Zhang J., Ali A.M., Lieu Y.K., Liu Z., Gao J., Rabadan R., Raza A., Mukherjee S, & Manley J.L. (2019). Disease-Causing Mutations in SF3B1 Alter Splicing by Disrupting Interaction with SUGP1. Molecular cell, 76(1), 82-95.e7.

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