Approximately 0.2 g of the sampled leaf was cut into small pieces with scissors, excluding the main veins. The leaf pieces were immersed in alcohol (96%, v/v) and kept at 4 °C in the dark until it turned white. The absorbance of the alcohol extract was determined at 649 and 665 nm using a UV-spectrophotometer (Hitachi UV-1800, Kyoto, Japan). Chlorophyll extraction and calculation of chlorophyll content were according to the method by Zhang et al.5 (link).
Uv 1800
Manufactured by Hitachi
Sourced in Japan
The UV 1800 is a spectrophotometer manufactured by Hitachi. It is designed to measure the absorbance of ultraviolet and visible light in liquid samples. The instrument is capable of scanning wavelengths from 190 to 1100 nanometers.
Automatically generated - may contain errors
5 protocols using uv 1800
1
Chlorophyll Extraction and Quantification
2
Comprehensive Characterization of N-Doped Graphene Quantum Dots
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In this work, all purchased reagents did not be further purified for experimental use. And, all the aqueous solutions were finished with ultrapure water before experiments. The morphology and microstructure of the materials were well-characterized by scanning electron microscopy (SEM, JEOL JSM 6700-F, Japan), X-ray diffraction (XRD, Siemens D5005, Germany), atomic force microscopic (AFM, Bruker, Multimode 8, America), transmission electron microscopy (TEM, JEOL, JEM-3010, Japan), Fourier transform infrared spectra (FT-IR, Nicolet Magna 5PC, America) X-ray photoelectron spectroscopy (XPS, Thermo Fisher Scientific, America), Raman spectra (Renishaw, inVia, England) and nitrogen adsorption (BEL, Sorpmax, Japan). The optical property of N-GQDs was investigated by UV-vis spectra (Shimadzu, UV-1800, Japan) and fluorescence spectra (Hitachi, F-7000, and Japan).
3
Measuring Antioxidant Capacity of Honey
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The antioxidant activity of honey samples was determined with ABTS method according to Re et al. (1999) (link). To generate the ABTS• radical, 2.5 ml of potassium persulfate solution (2.45 Mm) were added to 5 ml of ABTS solution (7 mM). The mixture was incubated at room temperature (25 ± 1 °C) for approximately 16 h. The stock solution was diluted with ethanol until an absorbance of 0.700 ± 0.02 at 734 nm. 4 ml ABTS •+ solution was mixed with 40 μl of honey solution (0.05 g/ml). The absorbance was measured spectrophotometrically (Hitachi, UV 1800, Japan) at room temperature (25 ± 1 °C) after 6 min. The antioxidant activity was expressed as mmol TE (Trolox Equivalent)/kg of honey.
4
Determination of Total Phenolic Content in Honey
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Total phenolic contents of honeys were determined according to the method of Akbulut et al. (2009) (link). One gram of honey was dissolved in 19.0 ml distilled water and then 0.5 ml of this solution was mixed with 2.5 ml of Folin-Ciocalteu's reagent solution (0.2 N) and 2.0 ml sodium carbonate solution (75 g/l). After 2 h dark incubation at room temperature (25 ± 1 °C), the absorbance was determined using a spectrophotometer (Hitachi, UV 1800, Japan) at a wavelength of 765 nm. Total phenolic content was expressed as mg gallic acid equivalent/100 g of honey sample.
5
Characterization of Metal Complexes
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The chemicals have been procured either from Sigma Aldrich or LOBA Chemie. Except for Pb(NO 3 ) 2 , CdCl 2 and HgCl 2 the other metal salts are sulfates. Double distilled water obtained from quartz double distillation plant were used to prepare metal salt solutions (0.01M). The FT-IR spectra were recorded as KBr pallet in Perkin Elmer RXI spectrometer. NMR spectra ( 1 H and 13 C) were obtained using Bruker Ultra Shield 300 MHz spectrophotometer using CDCl 3 or DMSO-d 6 as solvent. The UV/Visible spectra were recorded in Shimadzu UV 1800, uorescence in HITACHI 2700 and HRMS in WATERS Q-TOF premier mass spectrometer.
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