The following antibodies were used in this study: anti-FLAG (clone M2, WB 1:1,000; Sigma-Aldrich), anti-GAPDH (WB 1:5,000; UBPBio), FAK S910 (WB 1:1,000, 44-596G; Invitrogen), total FAK (WB 1:1,000, 610087; BD Biosciences), and paxillin (IF 1:500; Transduction Labs). Anti-HA (WB 1:500, IP 2 μg/1 mg cell lysate, SC F-7), anti–α-tubulin (WB 1:5,000, 23948), anti-Rab40c (WB 1:500, H-8 sc514826), cul-5 (WB 1:500, H-300), and mouse ANKRD28 (WB 1:500) were purchased from Santa Cruz Biotechnology. MOB1(E1N9D) and p-MOB1(D2F10) were purchased from Cell Signaling Technology. Rabbit anti-SAPS1/2/3, ANKRD28/52, and PP6c were purchased from Bethyl Laboratories. Specificity of anti-Rab40c antibody was confirmed by immunoblotting lysates derived from cells expressing FLAG-Rab40a, FLAG-Rab40b, or FLAG-Rab40c constructs (Fig S2A ).
Fak s910
Manufactured by Thermo Fisher Scientific
The FAK S910 is a laboratory equipment product from Thermo Fisher Scientific. It is a device used for sample preparation and analysis. The core function of the FAK S910 is to facilitate the effective processing and handling of samples in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Total fak, by BD (4 mentions)
Anti α tubulin, by Santa Cruz Biotechnology (4 mentions)
Anti rab40c, by Santa Cruz Biotechnology (4 mentions)
Anti ha, by Santa Cruz Biotechnology (4 mentions)
Anti flag clone m2, by Merck Group (2 mentions)
Cul 5, by Santa Cruz Biotechnology (2 mentions)
Mouse ankrd28, by Santa Cruz Biotechnology (2 mentions)
Mob1 e1n9d, by Cell Signaling Technology (2 mentions)
P mob1 d2f10, by Cell Signaling Technology (2 mentions)
Anti saps1 2 3, by Fortis Life Sciences (2 mentions)
Ankrd28 52, by Fortis Life Sciences (2 mentions)
Anti zyxin, by Abcam (2 mentions)
Anti eplin, by Cell Signaling Technology (2 mentions)
Anti eplin, by Proteintech (2 mentions)
Anti rab40b, by LSBio (2 mentions)
Anti β tubulin, by LI COR (2 mentions)
Anti e cadherin, by Cell Signaling Technology (2 mentions)
P fak y397, by Abcam (2 mentions)
Sir actin, by Cytoskeleton (2 mentions)
Anti flag, by Merck Group (2 mentions)
4 protocols using fak s910
1
Antibody Verification and Characterization
2
Antibody Validation for Cell Analysis
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The following antibodies were used in this study: anti-FLAG (WB 1:1,000, F3165; Sigma), anti-EPLIN (WB 1:1,000, IF 1:100, 50311; Cell Signaling Technology), anti-EPLIN (WB 1:1,000, IF 1:100, sc-136399; Santa Cruz Biotechnology), anti-EPLIN (WB 1:1,000, immunoprecipitation [IP] 1 μg/1 mg lysate, immunohistochemistry [IHC] 1:200, 16639–1-AP; Proteintech), anti-HA (WB 1:500, IP 2 µg/1 mg cell lysate, SC F-7; Santa Cruz Biotechnology), Alexa Fluor 568–phalloidin (IF 1:1,000, A1238; Life Technologies), SiR-actin (1:10,000, CY-SC001; Cytoskeleton), anti–β-tubulin (WB 1:2,500, 926–42211; LI-COR), anti–α-tubulin (WB 1:2,500, 23948; Santa Cruz Biotechnology), pFAK Y397 (WB 1:1,000, ab8129; Abcam), FAK S910 (WB 1:1,000, 44-596G; Invitrogen), anti–E-Cadherin (WB 1:1,000, IHC 1:200, 3195; Cell Signaling Technology), total FAK (WB 1:1,000, 610087; BD Biosciences), anti-zyxin (IF 1:1,000, ab50391; Abcam), anti-Rab40b (WB 1:500, LS-C353287; LSBio), anti-Rab40c (WB 1:500, H-8 sc-514826; Santa Cruz Biotechnology), and anti-CD63 (IF 1:100; gift from Andrew Peden, University of Shefield, Shefield, UK).
3
Antibodies for Cellular Imaging and Signaling
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Antibodies used in this study are as follows: anti-Flag -(WB 1:1000, Sigma F3165), anti-EPLIN -(WB 1:1000, IF 1:100, Cell Signaling Technology 50311) , anti-EPLIN (WB 1:1000, IF 1:100, Sant Cruz Biotechnology sc-136399), anti-EPLIN (WB 1:1000, IP 1 ug/1 mg lysate, IHC 1:200 Proteintech 16639-1-AP), anti-HA (WB 1:500, IP 2 μg/1mg cell lysate, Santa Cruz SC F-7) , Alexa-Fluor-568-phalloidin (IF 1:1000, Life Technologies A1238), SiR-Actin -(1:10,000, Cytoskeleton CY-SC001), anti-β-tubulin -(WB 1:2500, LiCor 926-42211), anti-α-tubulin -(WB 1:2500, Santa Cruz 23948), pFAK Y397 (WB 1:1000, Abcam ab8129), FAK S910 (WB 1:1000, Invitrogen 44-596G), anti-E-Cadherin (WB 1:1000, IHC 1:200, Cell Signaling 3195), total FAK (WB 1:1000, BD Biosciences 610087), anti-Zyxin (IF 1:1000 abcam, ab50391), anti-Rab40b (WB 1:500, LSBio, LS-C353287), anti-Rab40c (WB 1:500 Santa Cruz, H-8 sc-514826), anti-CD63 (IF 1:100, gift from Dr. Andrew Peden).
4
Antibody Characterization and Validation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The following antibodies were used in this study: anti-FLAG (clone M2, WB 1:1,000, Sigma), anti-GAPDH (WB 1:5,000, UBPBio), FAK S910 (WB 1:1,000, 44-596G, Invitrogen), total FAK (WB 1:1,000, 610087, BD Biosciences), paxillin (IF 1:500 Transduction labs). Anti-HA (WB 1:500, IP 2 µg/1 mg cell lysate, SC F-7), anti-α-tubulin (WB 1:5,000, 23948), anti-Rab40c (WB 1:500, H-8 sc514826), cul-5 (WB 1:500, H-300), and mouse ANKRD28 (WB 1:500) were purchased from Santa Cruz Biotechnology. MOB1(E1N9D) and p-MOB1(D2F10) were purchased from cell signaling technology. Rabbit anti-SAPS1/2/3, ANKRD28/52, PP6c were purchased from Bethyl Laboratories.
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