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2 protocols using folin ciocalteu reagent

1

Antifungal Potential of Polyphenols

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Distilled water (DW), dimethyl sulfoxide (DMSO), methanol (MeOH), ethyl acetate (EA), standard antifungals (amphotericin B and terbinafine), nutrient agar, and HPLC-grade polyphenols (vanillic acid, rutin, plumbagin, thymoquinone, gallic acid, catechin, syringic acid, coumaric acid, emodin, gentisic acid, caffeic acid, ferulic acid, luteolin, apigenin, myricetin, quercetin and kaempferol), 2,2-diphenyl-1-picryhydrazyl (DPPH), ascorbic acid, sulfuric acid, gallic acid, potassium acetate, quercetin, ammonium molybdate, aluminum chloride, trichloroacetic acid (TCA), potassium ferricyanide, and ferric chloride were purchased from Sigma Aldrich, United States. Phosphate buffer and Folin–Ciocalteu (FC) reagent were procured from Riedel-de Haen, Germany. Sabouraud dextrose agar (SDA) was purchased from Oxoid, England, Tween-80 from Merck-Schuchardt, United States, and RPMI-1640 was obtained from United traders, Rawalpindi, Pakistan.
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2

Phytochemical Extraction and Analysis

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Ethyl acetate, Methanol, Dimethyl sulfoxide (DMSO), and n-hexane were purchased from Sigma-Aldrich, Schnelldorf, Germany. Folin–Ciocalteu (FC) reagent was acquired from Riedel-de Haen, Germany. Quercetin, Potassium acetate, Gallic acid, and Aluminium chloride were purchased from Sigma Aldrich, Saint Louis, MO, USA. Rotary evaporator was acquired from Buchi, Flawil, Switzerl and while microplate reader was purchased from Biotech USA, microplate reader Elx 800. HPLC was acquired from Agilent Chem station Rev. B.02-01-SR1 (260), Boulder, Colorado, USA. Eppendorf tubes were taken from Merck, Kenilworth, NJ, USA.
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