Wpmy 1

Human normal prostate stromal immortalized cell line WPMY-1 (BNCC100291) and PCa cell lines PC-3 (BNCC337715), Du 145 (BNCC338240), LNCaP (BNCC337703), and 22RV1 (BNCC100161) were bought from BeNa Culture Collection (Shanghai, China). Cells were prepared at 37℃ with 5% CO₂. Culture medium information was: PC-3, Du 145, WPMY-1 cell lines in DMEM-H (BNCC338068) plus 10% FBS; LNCaP, 22RV1 cell lines in RPMI-1640 plus 10% FBS (BNCC341471). Du 145 cells were passaged in DMEM (Sigma-Aldrich) plus 10% dialyzed FBS (Gemini Bio-Products) in the methionine dependence assay. Control medium was supplemented with 100 µM l-methionine (Sigma-Aldrich), 100 µM l-cysteine (Fisher Scientific), 1.5 µM vitamin B12, and 4 mM l-glutamine. Also, 200 µM dl-homocysteine (Sigma-Aldrich) was added to the Met-Hcy+ medium. For sulfasalazine treatment, sulfasalazine (HY-14655, medchemexpress, USA) was purchased and dissolved in DMSO, and was added to the culture medium at a final concentration of 200 µM for treating PCa cells. The same amount of DMSO was supplemented to the control group for treatment [33 (link)].