Genomic DNA was extracted and purified from nine isolates (two from child 20, 26, and 30; and three from child 23) using the Wizard® Genomic DNA Purification according to the manufacturer’s instructions. Whole-genome sequencing (WGS) was carried out using the Oxford Nanopore Technology (ONT) Rapid Bar-coding SQK-RBK004 Sequencing protocol. Raw data was demultiplexed and removed from adapters using Porechop (https://github.com/rrwick/Porechop version 0.2.3_seqan2.1.1). Genome assembly was performed using the Flye assembler (version 0.2.3_seqan2.1.1) [15 (link)], while the genome annotation was carried out using Prokka (version 1.14.6) [18 (link)]. Acquired AMR genes were identified using ABRicate (version 1.0.1) [19 (link)]. ARG-ANNOT, Resfinder [20 (link)] and CARD databases [21 (link)] were used for the identification of resistance genes.
Ont rapid bar coding sqk rbk004 sequencing protocol
Manufactured by Oxford Nanopore
The ONT Rapid Bar-coding SQK-RBK004 Sequencing protocol is a laboratory equipment product developed by Oxford Nanopore Technologies. It is designed to enable rapid and efficient DNA sequencing. The core function of this protocol is to provide a streamlined approach for preparing DNA samples for nanopore-based sequencing, without elaborating on its intended applications.
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Lab products found in correlation
2 protocols using ont rapid bar coding sqk rbk004 sequencing protocol
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Nanopore Sequencing of Bacterial Isolates
2
Nanopore Sequencing of Bacterial Isolates
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Genomic DNA was extracted and purified from nine isolates (two from child 20, 26, and 30; and three from child 23) using the Wizard® Genomic DNA Purification according to the manufacturer’s instructions. Whole-genome sequencing (WGS) was carried out using the Oxford Nanopore Technology (ONT) Rapid Bar-coding SQK-RBK004 Sequencing protocol. Raw data was demultiplexed and removed from adapters using Porechop (https://github.com/rrwick/Porechop version 0.2.3_seqan2.1.1). Genome assembly was performed using the Flye assembler (version 0.2.3_seqan2.1.1) [15 (link)], while the genome annotation was carried out using Prokka (version 1.14.6) [18 (link)]. Acquired AMR genes were identified using ABRicate (version 1.0.1) [19 (link)]. ARG-ANNOT, Resfinder [20 (link)] and CARD databases [21 (link)] were used for the identification of resistance genes.
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