Imr 32

Human neuroblastoma cell lines SK-N-BE(2)-C (European Collection of Authenticated Cell Cultures, ECACC, Salisbury, UK) and IMR-32 (German Collection of Microorganisms and Cell Cultures, DSMZ, Darmstadt, Germany) were cultured under standard conditions in Dulbecco’s Modified Eagles Medium (DMEM containing L-glutamine and 4.5 g/L glucose, Gibco Invitrogen cell culture, Invitrogen, Paisley, UK) supplemented with 10% fetal calf serum (FCS; Sigma, St. Louis, MO, USA) and 1% non-essential amino acids (NEAA; Invitrogen, Carlsbad, CA, USA). All cell lines were regularly checked for mycoplasma and multiple contaminations (Multiplexion, Heidelberg, Germany) and routinely verified using DNA fingerprinting authentication by Multiplexion.

V79 hamster lung cells, which are recommended in the OECD toxicology guidelines for hazard identification (i.e., cytotoxicity and genotoxicity) of chemicals, were purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany). They were cultured in Dulbecco´s modified Eagle medium and Ham´s F12 medium (DMEM/F12) (PromoCell, Heidelberg, Germany) containing 10% fetal calf serum at 37 °C in a humidified atmosphere containing 5% CO₂. VC8 hamster cells were defective in DNA double-strand break (DSB) repair by homologous recombination (HR) due to the lack of functional BRCA2 protein [69 (link),70 (link)]. VC8 cells were provided by B. Kaina (Institute of Toxicology, Mainz, Germany). Nucleotide excision repair defective XPA and CSB deficient cells were also provided by B. Kaina and were described before [83 (link),84 (link)]. They were grown in DMEM containing 10% fetal calf serum. Human neuroblastoma cells (IMR-32 and SH-SY5Y) were purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany) and were used as a model of malignant cells. They were grown in DMEM or RPMI (Roswell park memorial institute) medium, respectively, containing 10% fetal calf serum. If not stated otherwise, HDACi were added to the exponentially growing cells and analyses were performed 6 h to 72 h later.

Human neuroblastoma cell lines SK-N-BE(2)-C (European Collection of Authenticated Cell Cultures, ECACC, Salisbury, UK), IMR-32 (German Collection of Microorganisms and Cell Cultures, DSMZ, Darmstadt, Germany), SK-N-AS (kindly provided by M. Schwab, DKFZ) and SH-SY5Y (DSMZ) as well as human medulloblastoma cell line MED8A (kindly provided by R. Gilbertson, St Jude Children’s Research Hospital, Memphis, TN, USA) and the non-transformed human foreskin fibroblast cell line VH7 (kindly provided by P. Boukamp, DKFZ) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Lonza, Basel, Switzerland) supplemented with 10% fetal calf serum (FCS, Sigma-Aldrich, Munich, Germany) and 1% non-essential amino acids (NEAA, Lonza). Kelly (DSMZ) and HD-MB03 (kindly provided by T. Milde, DKFZ) cells were cultured in RPMI 1640 medium (ThermoFisher Scientific, Braunschweig, Germany) containing 10% FCS and 1% NEAA. All cell lines were routinely authenticated using DNA fingerprinting authentication (DSMZ) and screened for mycoplasma contamination (Multiplexion, Heidelberg, Germany). All cell lines were cultured under standard conditions at 37 °C in a humidified atmosphere containing 5% CO₂ and passages 15–30 were used.