Vcam1 elisa kit

Manufactured by Thermo Fisher Scientific

Sourced in United States, Germany

The VCAM1 (Vascular Cell Adhesion Molecule 1) ELISA kit is a laboratory tool used to detect and quantify VCAM1 levels in various biological samples. VCAM1 is a cell surface glycoprotein involved in the adhesion of cells to the vascular endothelium. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to measure VCAM1 concentrations.

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Lab products found in correlation

3 nitrotyrosine elisa kit, by Abcam (3 mentions) Il 6 elisa kit, by Abcam (2 mentions) Tnf α elisa kit, by Abcam (1 mentions) Tgf β1 elisa kit, by Thermo Fisher Scientific (1 mentions) Hydrogen peroxide assay kit, by Abcam (1 mentions)

3 protocols using vcam1 elisa kit

Myocardial Oxidative and Inflammatory Markers

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Myocardial levels (n = 8–10 samples) of oxidative stress and inflammatory markers were tested using enzyme-linked immunosorbent assay (ELISA) and colorimetric assay kits: 3-nitrotyrosine ELISA kit (ab116691; Abcam), lipid peroxidation (malondialdehyde) assay kit (ab118970; Abcam), interleukin-6 (IL6) ELISA kit (ab100772; Abcam), intercellular cell adhesion molecule-1 (ICAM1) ELISA kit (ERICAM1; Thermo Fisher Scientific), vascular cell adhesion molecule-1 (VCAM1) ELISA kit (KHT0601; Thermo Fisher Scientific), and tumor necrosis factor alpha (TNFα) ELISA kit (ab108913; Abcam). Hydrogen peroxide (H₂O₂) was assessed in LV tissue homogenates (n = 8–10/group). Samples containing equal amounts of total protein were analyzed for H₂O₂ formation. H₂O₂ formation was measured by colorimetry at 540 nm. Results were converted using the standard curve for a known concentration of H₂O₂.

Kolijn D., Kovács Á., Herwig M., Lódi M., Sieme M., Alhaj A., Sandner P., Papp Z., Reusch P.H., Haldenwang P., Falcão-Pires I., Linke W.A., Jaquet K., Van Linthout S., Mügge A., Tschöpe C, & Hamdani N. (2020). Enhanced Cardiomyocyte Function in Hypertensive Rats With Diastolic Dysfunction and Human Heart Failure Patients After Acute Treatment With Soluble Guanylyl Cyclase (sGC) Activator. Frontiers in Physiology, 11, 345.

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Myocardial Oxidative Stress and Inflammation Assays

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Myocardial levels (n = 4–5 sample/group) of oxidative stress and inflammatory markers were tested with enzyme-linked immunosorbent assay (ELISA) and colorimetric assay kits: Hydrogen peroxide assay kit (ab102500; Abcam, Cambridge, UK), lipid peroxidation (malondialdehyde) assay kit (ab118970; Abcam), 3-Nitrotyrosine ELISA kit (ab116691; Abcam), nitric oxide (NO) colorimetric assay kit (K262; BioVision, Inc., Milpitas, CA, USA), interleukin-6 (IL-6) ELISA kit (ab100772; Abcam), intercellular cell adhesion molecule-1 (ICAM-1) ELISA kit (ERICAM1; Thermo Fisher Scientific, Waltham, MA, USA), vascular cell adhesion molecule-1 (VCAM-1) ELISA kit (KHT0601; Thermo Fisher Scientific), tumor necrosis factor alpha (TNFα) ELISA kit (ab108913; Abcam), transforming growth factor-beta 1 (TGF-β1) ELISA kit (BMS623-3; Thermo Fisher Scientific), fibronectin 1 (FN-1) ELISA kit (LS-F2425; LifeSpan BioSciences, Seattle, WA, USA).

Kovács Á., Herwig M., Budde H., Delalat S., Kolijn D., Bódi B., Hassoun R., Tangos M., Zhazykbayeva S., Balogh Á., Czuriga D., Van Linthout S., Tschöpe C., Dhalla N.S., Mügge A., Tóth A., Papp Z., Barta J, & Hamdani N. (2021). Interventricular Differences of Signaling Pathways-Mediated Regulation of Cardiomyocyte Function in Response to High Oxidative Stress in the Post-Ischemic Failing Rat Heart. Antioxidants, 10(6), 964.

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Oxidative Stress and Inflammation in End-Stage Heart Failure

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Oxidative stress and inflammatory parameters of end-stage human failing hearts (n = 8–10 samples) were measured before and after treatment with GSH using colorimetric assay and enzyme-linked immunosorbent assay kits (ELISA). The following kits were used for the measurements: lipid peroxidation (malondialdehyde) assay kit (ab118970; Abcam, Cambridge, UK), 3-nitrotyrosine ELISA kit (ab116691; Abcam, Cambridge, UK), interleukin-6 (IL-6) ELISA kit (ab100772; Abcam, Cambridge, UK); intercellular cell adhesion molecule-1 (ICAM1) ELISA kit (ERICAM1; Thermo Fisher Scientific, Darmstadt, Germany), vascular cell adhesion molecule-1 (VCAM1) ELISA kit (KHT0601; Thermo Fisher Scientific, Darmstadt, Germany); and tumor necrosis factor alpha (TNFα) ELISA kit (ab108913; Abcam, Cambridge, UK). To analyze the hydrogen peroxide (H₂O₂) level in LV tissue homogenates, a colorimetric assay was used and the H₂O₂ formation was measured at 540 nm. For the calculation, a standard curve with a known concentration of H₂O₂ was used.

Budde H., Hassoun R., Tangos M., Zhazykbayeva S., Herwig M., Varatnitskaya M., Sieme M., Delalat S., Sultana I., Kolijn D., Gömöri K., Jarkas M., Lódi M., Jaquet K., Kovács Á., Mannherz H.G., Sequeira V., Mügge A., Leichert L.I., Sossalla S, & Hamdani N. (2021). The Interplay between S-Glutathionylation and Phosphorylation of Cardiac Troponin I and Myosin Binding Protein C in End-Stage Human Failing Hearts. Antioxidants, 10(7), 1134.

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