Vitamin D levels in the plasma samples were measured using the LIAISON® 25 OH Vitamin D TOTAL Assay (DiaSorin, Saluggia, Italy) and LIAISON® analyser, following the manufacturer's instructions. The final measurements were indicated in nanograms per millilitre.
Liaison analyser
Manufactured by DiaSorin
Sourced in Italy
The Liaison analyser is a diagnostic instrument developed by DiaSorin for the detection and quantification of various analytes in biological samples. It employs chemiluminescent technology to perform immunoassay analyses. The core function of the Liaison analyser is to provide accurate and reliable results for clinical diagnostic tests.
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Lab products found in correlation
7 protocols using liaison analyser
1
Plasma Vitamin D Quantification
2
Detecting Specific Antibodies via Chemiluminescence
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Specific antibodies were detected with chemiluminescence immunoassay tests for HSV-1/2 IgM, HSV-1/2 IgG, HSV-1 IgG, and HSV-2 IgG (DiaSorin, Saluggia, Italy) using a Liaison analyser (DiaSorin).
3
Oral Glucose Tolerance Test Insulin Resistance Evaluation
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Seventy-five gramm OGTT (0′-30′-120′) was performed after an overnight fasting period of at least 10 h. Plasma glucose (PG), HbA1c, and other routine laboratory parameters were determined according to standard protocols and the International Federation of Clinical Chemistry (IFCC) standards. Serum insulin levels were measured using Liaison analyser (DiaSorin, Saluggia, Italy). HOMA2-IR, Triglyceride-Glucose index (TyG) as surrogate measures of insulin resistance and HOMA2-B values as marker of β-cell function were calculated [35 (link)]. TyG index was calculated as: TyG = ln[ 38,67*Tg(mg/dl)*18*glucose(mg/dl)/2] [36 (link)–38 (link)]. In order to assess the future T2DM development risk we used the following markers: Disposition Index basal (DIbasal: HOMA2-B x 1/HOMA2-IR) [39 (link), 40 (link)], Insulin Secretion and Sensitivity Index-2 (ISSI-2: using the formula: AUCInsulin/AUCGlucose x Matsuda) [41 (link)].
4
EBV Viral Capsid Antigen IgG Quantification
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EBV viral capside antigens IgG was quantified in the investigated samples by the LIAISON analyser, following the manufacturer's instructions (DiaSorin, Dartford, UK). The samples were tested in duplicates, along with calibrator and control samples, to validate the assay. The fully automated LIAISON immunoassay was used with its magnetic microparticle technology, chemiluminescence with flashlight kinetics, and an isoluminol derivative as labels. Of each sample, 30 µL were used for this analysis. All reagents required for the assay (magnetic particles, luminescence-labelled tracer, two calibrators, diluent, and assay buffer) were provided ready to use and assembled in one integrated reagent cartridge identifiable by a bar-coded label providing information such as lot number, expiry date, and calibration data. The analyser automatically calculated the antibody concentrations and expressed it as U/ml.
5
Vitamin D Status in Chronic Diseases
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Fasting venous blood samples were obtained from all patients following a fasting
period of 8 hours to determine laboratory parameters. Serum 25-(OH) vitD levels
were measured by chemiluminescent immunoassay using a LIAISON analyser (DiaSorin
Inc). VitD deficiency was defined as serum level of 25-(OH) vitD <20
ng/ml.
Patients with DM were identified on admission as those with documented DM using
either oral hypoglycemic agents or insulin treatment. HT was defined as blood
pressure >140/90 mmHg or use of antihypertensive therapy on admission.
Hyperlipidemia (HL) was defined as total cholesterol <200 mg/dl or use of
antihyperlipidemic therapy on admission. Chronic obstructive pulmonary disease
was defined as forced expiratory volume in 1 second/forced vital capacity
(FEV1/FVC) <70% and FEV1 >80% of predicted value. Heart failure diagnosis
was based on clinical features and echocardiographic results. Patients with
clinical features of heart failure or whose left ventricular ejection fraction
were <50% were excluded from the study. Renal failure was defined as a serum
creatinine level >1.5 mg/dl.
period of 8 hours to determine laboratory parameters. Serum 25-(OH) vitD levels
were measured by chemiluminescent immunoassay using a LIAISON analyser (DiaSorin
Inc). VitD deficiency was defined as serum level of 25-(OH) vitD <20
ng/ml.
Patients with DM were identified on admission as those with documented DM using
either oral hypoglycemic agents or insulin treatment. HT was defined as blood
pressure >140/90 mmHg or use of antihypertensive therapy on admission.
Hyperlipidemia (HL) was defined as total cholesterol <200 mg/dl or use of
antihyperlipidemic therapy on admission. Chronic obstructive pulmonary disease
was defined as forced expiratory volume in 1 second/forced vital capacity
(FEV1/FVC) <70% and FEV1 >80% of predicted value. Heart failure diagnosis
was based on clinical features and echocardiographic results. Patients with
clinical features of heart failure or whose left ventricular ejection fraction
were <50% were excluded from the study. Renal failure was defined as a serum
creatinine level >1.5 mg/dl.
6
Vitamin D Supplementation for CABG Surgery
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25-hydroxy (OH) vitD, calcium, and other biochemical and hematologic parameter
levels were measured following a fasting period of 8 hours. Serum 25-(OH) vitD
levels were measured by chemiluminescent immunoassay using a LIAISON analyser
(DiaSorin Inc). VitD deficiency was defined as serum levels of 25-(OH) vitD
<20 ng/ml. VitD insufficiency was defined as a level of 20-29 ng/ml. Plasma
levels of 25-(OH) vitD >30 ng/ml were defined as normal. Oral vitD
supplementation (50.000 U) was initiated 48 hours before CABG surgery.
levels were measured following a fasting period of 8 hours. Serum 25-(OH) vitD
levels were measured by chemiluminescent immunoassay using a LIAISON analyser
(DiaSorin Inc). VitD deficiency was defined as serum levels of 25-(OH) vitD
<20 ng/ml. VitD insufficiency was defined as a level of 20-29 ng/ml. Plasma
levels of 25-(OH) vitD >30 ng/ml were defined as normal. Oral vitD
supplementation (50.000 U) was initiated 48 hours before CABG surgery.
7
Mycoplasma pneumoniae Antibody Detection
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Samples were sent to the Department of Laboratory Medicine, Munich (LMU) and stored at -80 • C until analysis. Serum IgG against M. pneumoniae were measured in the ISO 15189 accredited lab on a DiaSorin Liaison analyser using Chemiluminescence Immunoassay with paramagnetic microparticle solid phase (CLIA) technology; the analyser range was 1-200 AU/ml. M. pneumoniae elicits antibody responses after around one week of illness. This is initially an IgM response before seroconversion to IgG; serum IgG tends to peak at 3-6 weeks, before it gradually declines (Atkinson et al., 2008) . IgG tends to remain elevated in children for around four years following infection but can also persist indefinitely (Daxboeck et al., 2003; Jacobs et al., 1986; Foy et al., 1977) . Serum IgG ≥10 AU/ml was considered positive for previous infection with M. pneumoniae at any point in time and <10 AU/ml as negative (Waris et al., 1998; Almasri et al., 2011) . IgG positivity is most likely due to past, rather than acute, infection with M. pneumoniae and does not provide information on how recently the participant was infected.
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