Log In Sign up
The largest database of trusted experimental protocols

CD278, also known as CTLA-4, is a cell surface receptor that plays a crucial role in the regulation of T-cell activation and immune response. It functions as an inhibitory receptor, delivering a negative signal that downregulates T-cell activation and helps maintain immune homeostasis.

Automatically generated - may contain errors

Lab products found in correlation

Cd152, by BD (1 mentions) Xcelligence real time cell analysis system, by Agilent Technologies (1 mentions) Siglec f, by BioLegend (1 mentions) Apc conjugated streptavidin, by Thermo Fisher Scientific (1 mentions) Fcεri, by BioLegend (1 mentions) Percp cy5, by BD (1 mentions) Ctla 4, by BioLegend (1 mentions) Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions) Percp cy5, by Thermo Fisher Scientific (1 mentions) Fluorescein isothiocyanate (fitc), by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Ammonium chloride, by Merck Group (1 mentions) Facscanto 2, by BD (1 mentions) Lsrfortessa, by BD (1 mentions) Cd11b, by BioLegend (1 mentions) F4 80, by BioLegend (1 mentions)

2 protocols using cd278

1

In vitro Cytotoxicity Assay for CD3 EDG Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
In vitro cytotoxicity assays (TDCC) were monitored by xCELLigence Real-Time Cell Analysis (RTCA) system (ACEA Biosciences). The target cells (2.5 × 103 cells) and spleen cells (1 × 105 cells) from human CD3 EDG–replaced mice and wild-type (C57BL/6) mice were mixed with or without TRAB for 72 hours. The percentage of cytotoxicity was calculated as follows:

where A was the mean cell index of wells without TRAB; B was the mean cell index of wells with TRAB. Baseline was normalized as 1.
At the end of incubation, the cells were collected from the plate. Cells were stained with mouse CD4 (BD Horizon), CD8, CD25, CD69, CD152 (BD Pharmingen), and CD278 (eBioscience) and analyzed by flow cytometer.
Ueda O., Wada N.A., Kinoshita Y., Hino H., Kakefuda M., Ito T., Fujii E., Noguchi M., Sato K., Morita M., Tateishi H., Matsumoto K., Goto C., Kawase Y., Kato A., Hattori K., Nezu J., Ishiguro T, & Jishage K.I. (2017). Entire CD3ε, δ, and γ humanized mouse to evaluate human CD3–mediated therapeutics. Scientific Reports, 7, 45839.
+ Open protocol
+ Expand
2

Comprehensive Mouse Spleen and Bone Marrow Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Briefly, mouse spleens and BM were collected and dissociated through a 40μm strainer to produce a single cell suspension. Red blood cells were lysed using 0.86% ammonium chloride (Sigma). Cells were counted, washed in flow cytometry buffer (1% BSA (Sigma), 2mM EDTA (Invitrogen) in PBS. FcR were blocked with 2.4G2 (BioXCell) and Rat IgG (Invitrogen) while staining with McAb at 40 for 30 minutes. Intracellular staining was carried out using the eBioscience kit. Samples were acquired using a FACSCanto II or LSRFortessa flow cytometer (BD Biosciences) and analyzed with FlowJo software (Tree Star, Inc.). The following mAb against mouse antigens were used as FITC, PE, PerCP-Cy5.5, PE-Cy7, allophycocyanin (APC), APC-ef780, Pacific blue, AF450, AF700, PE Texas Red, or biotin conjugates: FcεRI, AA4.1, CD23, CD43, IgM, FcγRII/III, ckit, Sca-1, CD34, CD138, CD45R (B220; RA3-6B2), CD278 (ICOS; C398.4A), IgD (11–26) (eBioscience), CD4 (RM4–5), CD8a (53–6.7), CD95 (Fas; Jo2), CXCR5 (2G8) (BD Biosciences), CD3 (17A2), CD19 (6D5), Foxp3, CTLA-4, Siglec F, CD11b, F4/80, TCRβ, Gr-1, FcεRI, and CD279 (PD-1; J43) (Biolegend). Biotinylated antibodies were detected using PerCP-Cy5.5– (BD Biosciences) or APC-conjugated streptavidin (eBioscience). FITC or biotin-conjugated PNA was obtained from Vector Laboratories. Plots shown are on a Logicle scale.
Zaretsky A.G., Konradt C., Dépis F., Wing J.B., Goenka R., Atria D.G., Silver J.S., Cho S., Wolf A.I., Quinn W.J., Engiles J.B., Brown D.C., Beiting D., Erikson J., Allman D., Cancro M.P., Sakaguchi S., Lu L., Benoist C.O, & Hunter C.A. (2017). T Regulatory Cells Support Plasma Cell Populations in the Bone Marrow. Cell reports, 18(8), 1906-1916.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!