Thiazolyl blue mtt

Glycerol (Gly, 99+% extra pure, Acros Organics, Geel, Belgium) and divinyl sulfone (DVS, >96%, TCI) were used as received in p(QC) particle preparation. Quercetin dihydrate (QC, 99.85% MP Biomedicals) was used as active agent. Folin–Ciocalteau’s phenol reagent (FC, Sigma-Aldrich), gallic acid (GA, 97.5–102.5%, Aldrich), Sodium nitrite (Merck, extra pure), and aluminum chloride (Merck, anhydrous powder sublimed from synthesis) were used for antioxidant testing.
L929 fibroblast cell line (Mouse C3/An connective tissue), obtained from the SAP Institute (Ankara, Turkey), was used for the cell viability tests. Dulbecco’s Modified Eagle’s Medium, DMEM, 4.5 g/L Glucose, sodium pyruvate, L-Glutamine, 3.7 g/L NaHCO₃ (Pan Biotech GmbH, Aidenbach, Germany) was used as received. Fetal bovine serum (FBS Premium, heat inactivated), antibiotics (penicillin (10,000 U/mL), streptomycin (10,000 μg/mL), and trypsin/EDTA (trypsin 0.25%, 1 mM EDTA-Na₄ in HBSS) were purchased from Pan Biotech (GmbH, Germany). Trypan Blue solution (0.5%) was acquired from Biological Industries and thiazolyl blue (MTT) was obtained from BioFroxx (Einhausen, Germany). Dimethyl sulfoxide (DMSO, 99.9%, Carlo-Erba) was used as received.

Curcumin (purity higher than 98%) and DMEM medium were purchased from Shanghai Adamas Reagent Co., Ltd Pluronic^®F127 (F127) and Pluronic^®F68 (F68) were purchased from BASF SE (Germany). Growth factor-reduced Matrigel was purchased from Corning Incorporated (USA). Recombinant Bovine bFGF, Trizol and BCA Protein Assay Kit were purchased from Beyotime Biotechnology. hESC and HUVEC cell lines were purchased from ATCC. Thiazolyl Blue (MTT, purity higher than 98%) was purchased from Biofroxx (Germany), DMSO was purchased from Aladdin Reagent Shanghai Co., Ltd and Endothelial Cell Medium (ECM) was purchased from ScienCell (USA). Fetal bovine serum (FBS) was purchased from Zhejiang Tianhang Biotechnology Co., Ltd anti-TGF-β1 (21898-1-AP, Proteintech, Rosemont, IL, USA). Cyanine7 dye was synthesized in our laboratory. Cross-linked sodium hyaluronate gel for the uterine cavity (HA-gel) was purchased from Changzhou Bioregen Biomedicine Co., Ltd.