Colibri 7 led

Manufactured by Zeiss

Sourced in Germany

The Colibri 7 LEDs is a lab equipment product by Zeiss. It is a light source that utilizes seven light-emitting diodes (LEDs) to provide illumination for various applications in the laboratory setting. The core function of the Colibri 7 LEDs is to generate and deliver light to enable visualization, analysis, and other research-related tasks.

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Lab products found in correlation

Zen blue software, by Zeiss (6 mentions) Axio observer, by Zeiss (3 mentions) Prime 95b scmos camera, by Teledyne (3 mentions) Axiovision software, by Zeiss (2 mentions) Axiocam mrm, by Zeiss (2 mentions) Axio imager, by Zeiss (2 mentions) Axio imager z2m microscope, by Zeiss (1 mentions) Axiocam 506 mono, by Zeiss (1 mentions)

Spelling variants of this product

Colibri 7 (44 citations) Colibri (16 citations) Colibri 7 LED light source (6 citations) Colibri 7 LED illumination system (3 citations) Colibri 7 LED 298 illumination (2 citations) Colibri 7 LED illumination (2 citations)

6 protocols using colibri 7 led

Measuring Kinesin Ciliary Localization

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Nematodes were mounted as above. Widefield images were acquired on a Zeiss Axio Observer with Colibri 7 LEDs and ZenBlue software (Carl Zeiss Microscopy, Oberkochen, Germany) using a Photometrics Prime 95B sCMOS camera (Teledyne Photometrics, Tucson, AZ). A 100x/1.4 Oil Plan-Apochromat objective was used for imaging the phasmid cilia of Day 1 hermaphrodite animals. Images were imported into Fiji/ImageJ [105 ] and the length of KAP-1::GFP labeling was measured for each cilium using maximum intensity projections. Length was measured starting at the transition zone, which is distinct and bright, and ending when fluorescence was no longer visible. Kruskal-Wallis one-way ANOVA analysis and posthoc Dunn’s multiple comparison test were performed in Prism (Graphpad Software).
We also wanted to examine if ttll-4 suppressed the ccpp-1 KAP-1::GFP span defect, but were unable to obtain a viable ccpp-1;ttll-4;Ex[kap-1::gfp +pRF4] strain, possibly due to synthetic lethality with the overexpressed kap-1::gfp reporter.

Power K.M., Akella J.S., Gu A., Walsh J.D., Bellotti S., Morash M., Zhang W., Ramadan Y.H., Ross N., Golden A., Smith H.E., Barr M.M, & O’Hagan R. (2020). Mutation of NEKL-4/NEK10 and TTLL genes suppress neuronal ciliary degeneration caused by loss of CCPP-1 deglutamylase function. PLoS Genetics, 16(10), e1009052.

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Widefield Imaging of Nematodes

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Power K.M., Nguyen K.C., Silva A., Singh S., Hall D.H., Rongo C, & Barr M.M. (2024). NEKL-4 regulates microtubule stability and mitochondrial health in C. elegans ciliated neurons. bioRxiv.

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Quantifying Ciliary KAP-1 Localization

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Nematodes were mounted as above. Widefield images were acquired on a Zeiss Axio Observer with Colibri 7 LEDs and ZenBlue software (Carl Zeiss Microscopy, Oberkochen, Germany) using a Photometrics Prime 95B sCMOS camera (Teledyne Photometrics, Tucson, AZ). A 100x/1.4 Oil Plan-Apochromat objective was used for imaging the phasmid cilia of Day 1 hermaphrodite animals. Images were imported into Fiji/ImageJ [94] and the length of KAP-1::GFP labeling was measured for each cilium using maximum intensity projections. Length was measured starting at the transition zone, which is distinct and bright, and ending when fluorescence was no longer visible. Kruskall-Wallis one-way ANOVA analysis and posthoc Dunn's multiple comparison test were performed in Prism (Graphpad Software).
We also wanted to examine if ttll-4 suppressed the ccpp-1 KAP-1::GFP span defect, but were unable to obtain a viable ccpp-1;ttll-4; kap-1::gfp strain, possibly due to synthetic lethality with the overexpressed kap-1::gfp reporter.

Power K.M., Akella J.S., Gu A., Walsh J.D., Bellotti S., Morash M., Zhang W., Ross N., Golden A., Smith H.E., Barr M.M., & O’Hagan R. (2020). Mutation of NEKL-4/NEK10 and TTLL genes opposes loss of the CCPP-1 deglutamylase and prevents neuronal ciliary degeneration.

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Automated Fluorescence Microscopy Imaging

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Images were recorded on a Zeiss AxioImager.Z2m microscope with a cooled charged-coupled device (CCD) camera (Zeiss AxioCam MRm, Zeiss Oberkochen, Germany). The microscope was equipped with a Zeiss Colibri 7 LED (385 nm for DAPI, 469 nm for Alexa 488 dye, and 590 nm for autofluorescence) and a Multi Zeiss 62 HE filter cube (Beam splitter FT 395 + 495 + 610). The Zeiss AxioVision software (Zeiss, Germany) was used for automated image acquisition with a custom-built macro (23 (link), 67 (link), 68 (link)). The focal planes of 120 fields of view per sample were identified with 1× magnification. Subsequent fine-tuning and image recording were done with a 63x Plan Apochromat objective (1.4 numerical aperture, oil immersion).

Brüwer J.D., Orellana L.H., Sidhu C., Klip H.C., Meunier C.L., Boersma M., Wiltshire K.H., Amann R, & Fuchs B.M. (2023). In situ cell division and mortality rates of SAR11, SAR86, Bacteroidetes, and Aurantivirga during phytoplankton blooms reveal differences in population controls. mSystems, 8(3), e01287-22.

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Fluorescent Microscopy Signal Acquisition

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For signal acquisition we did 13 detection cycles, using a Zeiss Axio Observer Z.2 fluorescent microscope (Carl Zeiss Microscopy, GmbH) with a Colibri 7 LED light source (Carl Zeiss Microscopy, GmbH, 423052-9770-000), equipped with a Zeiss 20×/0.75 Plan-Apochromat, a Zeiss AxioCam 506 Mono digital camera and an automated stage, that allowed imaging of the same regions in every cycle. For signal detection, we used the following Chroma filters: DAPI (49000), FITC (49003), Cy3 (49304), Cy5 (49307), Texas Red (49310) and Atto740 (49007).

Sountoulidis A., Marco Salas S., Braun E., Avenel C., Bergenstråhle J., Theelke J., Vicari M., Czarnewski P., Liontos A., Abalo X., Andrusivová Ž., Mirzazadeh R., Asp M., Li X., Hu L., Sariyar S., Martinez Casals A., Ayoglu B., Firsova A., Michaëlsson J., Lundberg E., Wählby C., Sundström E., Linnarsson S., Lundeberg J., Nilsson M, & Samakovlis C. (2023). A topographic atlas defines developmental origins of cell heterogeneity in the human embryonic lung. Nature Cell Biology, 25(2), 351-365.

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Multi-Probe Fluorescence Microscopy

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Samples were imaged on a Zeiss AxioImager.Z2m, equipped with a charged-coupled device (CCD) camera (Zeiss AxioCam MRm, Zeiss, Oberkochen, Germany), and illuminated with a Zeiss Colibri 7 LED (excitation: 385 nm for DNA, 469 nm for 16S rRNA CARD-FISH, and 590 nm for direct-geneFISH signals). The microscope was equipped with a multi-Zeiss 62 HE filter cube (Beam splitter FT 395 + 495 + 610). Images were recorded with a custom-built macro^{45 (link),46 (link)} within the Zeiss AxioVision software (Zeiss, Germany). A total of 120 fields of view per sample were recorded with a 63x Plan Apochromat objective (1.4 NA, oil immersion). For high-resolution imaging, we used a Zeiss LSM 780 (Zeiss, Oberkochen, Germany), with an ELYRA PS.1 detector upgrade. The microscope was equipped with a 63x plan apochromatic oil immersion objective and the excitation lasers 405 nm (DAPI), 488 nm (16S rRNA CARD-FISH), and 591 nm (direct-geneFISH).

Brüwer J.D., Sidhu C., Zhao Y., Eich A., Rößler L., Orellana L.H, & Fuchs B.M. (2024). Globally occurring pelagiphage infections create ribosome-deprived cells. Nature Communications, 15, 3715.

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