Transwell plates 8 μm pores

Manufactured by Corning

Transwell plates (8-μm pores) are a type of cell culture insert that facilitates the study of cell migration and invasion. These plates feature a membrane with 8-micrometer pores, which allow cells to migrate through the membrane. This feature enables researchers to analyze cellular behaviors related to migration and invasion in a controlled in vitro environment.

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Lab products found in correlation

Matrigel, by BD (2 mentions)

Close spelling variants of this product

Transwell plates with 8-μm pores 24-well (8 μm pores) transwell plates 8-μm pores Pore transwells Transwell plates

2 protocols using transwell plates 8 μm pores

Transwell-Based Cell Migration and Invasion Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Transwell plates (8-μm pores) (Costar/Corning, Lowell, MA) were used for the Transwell migration or invasion assays. 5 × 10⁴ or 1 × 10⁵ cells resuspended in serum-free medium were placed in the upper chamber of each insert, either uncoated or coated with Matrigel (BD Biosciences). The lower chamber contained culture medium with 10% FBS to act as a chemoattractant. The cells were incubated for 12 h or 24 h, and were then fixed and stained. Cells on the undersides of the filters were observed and counted under 200× magnification.

Zhang J., Zheng Z.Q., Yuan Y.W., Zhang P.P., Li Y.Q., Wang Y.Q., Tang X.R., Wen X., Hong X.H., Lei Y., He Q.M., Yang X.J., Sun Y., Ma J, & Liu N. (2019). NFAT1 Hypermethylation Promotes Epithelial-Mesenchymal Transition and Metastasis in Nasopharyngeal Carcinoma by Activating ITGA6 Transcription. Neoplasia (New York, N.Y.), 21(3), 311-321.

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Transwell Cell Migration and Invasion Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Transwell plates (8-μm pores) (Costar/Corning, Lowell, MA) were used for Transwell migration or invasion assays. 5 × 10⁴ (migration assay) or 1 × 10⁵ (invasion assay) cells resuspended in serum-free medium were placed in the upper chamber of each insert, either uncoated or coated with Matrigel (BD Biosciences). The lower chamber contained culture medium with 10% FBS to act as a chemoattractant. The cells were incubated for 12 or 24 h and were then fixed and stained. Cells on the undersides of the filters were observed and counted under 200× magnification.

Zhang J., Luo J., Jiang H., Xie T., Zheng J., Tian Y., Li R., Wang B., Lin J., Xu A., Huang X, & Yuan Y. (2019). The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing. Frontiers in Oncology, 9, 1214.

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