H 2 db kh95

Manufactured by BD

Sourced in United States

The H-2 Db (KH95) is a lab equipment product. It is a core function product for laboratory use.

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Lab products found in correlation

70 μm cell strainer, by Thermo Fisher Scientific (2 mentions) H 2kk 36 7 5, by BioLegend (2 mentions) H 2 dk 15 5 5, by BioLegend (2 mentions) Facscalibur, by BD (2 mentions) H 2kb af6 88.5, by BD (1 mentions) Pd l1 mih5, by Thermo Fisher Scientific (1 mentions) Hla abc w6 32, by BioLegend (1 mentions) Cd3 17a2, by Thermo Fisher Scientific (1 mentions) Cd4 gk1, by BioLegend (1 mentions) Cd62l mel 14, by Thermo Fisher Scientific (1 mentions) Cd44 im7, by BioLegend (1 mentions) Cd8a 53 6, by BioLegend (1 mentions) 7 aad, by Thermo Fisher Scientific (1 mentions) Lsrfortessa x 20, by BD (1 mentions) Facsverse flow cytometer, by BD (1 mentions) Fixable viability dye, by Thermo Fisher Scientific (1 mentions) Foxp3 staining buffer set, by Thermo Fisher Scientific (1 mentions)

4 protocols using h 2 db kh95

Multiparametric flow cytometry for immune cells

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Samples were blocked with 2% normal mouse serum. Fixable yellow (L34959, Invitrogen, Carlsbad, CA, USA) or 7AAD (Cat 00699350, eBioscience, Waltham, MA, USA) was used to stain live/dead cells. Anti-mouse antibodies used were CD3 (17A2, eBioscience), CD4 (GK1.5, BioLegend, San Diego, CA, USA), TCR Vα2 (KB5-C20, BD Pharmingen), CD8a (53-6.7, BioLegend), CD44 (IM7, Biolegend), CD62L (MEL-14, eBioscience), H-2Db (KH95, BD Pharmingen), H-2Kb (AF6–88.5, BD Pharmingen), PD-L1 (MIH5, eBioscience). Anti-human antibodies used were HLA ABC (W6/32, Biolegend), PD-L1 (29E.283, Biolegend), anti-mouse IgG2a K Isotype (Biolegend), and anti-mouse IgG2b K (Biolegend).
Fluorescence was measured on BD LSR Fortessa X-20 or BD FACSVerse flow cytometer (BD Biosciences, North Ryde, NSW, Australia) and data analyzed using the FlowJo, LLC software (BD Biosciences, Franklin Lakes, NJ, USA).

Lau P.K., Cullinane C., Jackson S., Walker R., Smith L.K., Slater A., Kirby L., Patel R.P., von Scheidt B., Slaney C.Y., McArthur G.A, & Sheppard K.E. (2021). Enhancing Adoptive Cell Transfer with Combination BRAF-MEK and CDK4/6 Inhibitors in Melanoma. Cancers, 13(24), 6342.

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Comprehensive Immune Cell Profiling

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Cells were typed using biotin- or fluorochrome-conjugated antibodies with specificity for CD45 (30-F11), NK1.1 (PK138), CD11b (M1/70), CD49a (HMα1), CD49b (DX5), IFN-γ (XMG1.2), KLRG1 (MAFA), CD3ϵ (145-2C11), NKp46 (29A1.4), CD107a (1D4B), and Ki-67 (B56), Eomes (Dan11mag), Ly49C (4LO3311), Ly49I (YLI-90), NKG2A (16a11), PVR (TX56), B2M (S19.8), H2-Db (KH95), H2-Kb (AF6-88.5), Qa1-b (6A8.6F10.1A6), pan-cytokeratin (C11), Rae pan (REA273), purchased from BD PharMingen, BioLegend, Invitrogen, Miltenyi or eBioscience. Clone 4LO3311 was a gift by Jennifer Laurent. Dead cells were excluded using fixable viability dyes (eBioscience) and the Foxp3 staining buffer set (eBioscience) was used for detection of intracellular and intranuclear antigens.

Depierreux D.M., Kieckbusch J., Shreeve N., Hawkes D.A., Marsh B., Blelloch R., Sharkey A, & Colucci F. (2022). Beyond Maternal Tolerance: Education of Uterine Natural Killer Cells by Maternal MHC Drives Fetal Growth. Frontiers in Immunology, 13, 808227.

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Murine MHC Typing by Flow Cytometry

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MHC typing was performed by flow cytometry. Spleens were pressed through a 70 μm cell strainer (Thermo Fisher Scientific, Ottawa, Ontario, Canada). Spleen cell suspensions were treated with NH₄Cl to lyse red blood cells. Single cell suspensions were stained with the following antibodies: I-A^k (10-3.6), I-A^k (11-5.2), I-A^b (AF6-120.1), I-E (14.4.4S), H-2K^k (36-7-5), H-2 D^k (15-5-5)(Biolegend, San Diego, CA, USA) and H-2 D^b (KH95; BD Biosciences, Mississauga, Ontario, Canada), Flow cytometry data were acquired on an FACSCalibur instrument (BD Biosciences) and analyzed with FlowJo software (TreeStar, Ashland, OR, USA).

Pelletier A.N., Aliesky H.A., Banuelos B., Chabot-Roy G., Rapoport B., Lesage S, & McLachlan S.M. (2015). Evidence that MHC I-E dampens thyroid autoantibodies and prevents spreading to a second thyroid autoantigen in I-Ak NOD mice. Genes and immunity, 16(4), 268-274.

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Murine MHC Typing by Flow Cytometry

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