Classic c25kc incubator shaker

The lipophilicities of the complexes were determined using a modified “shake flask” method. A solution of 1-octanol saturated with water was prepared by combining 16 mL of 1-octanol (99.9%) with 4 mL of deionized water (MilliQ). A solution of water saturated with 1-octanol was prepared from 16 mL of deionized water (MilliQ) and 4 mL of 1-octanol (99.9%). The two saturated solutions were shaken at room temperature for 24 h (New Brunswick Classic C25KC Incubator Shaker set at 230 rpm). The C^N complexes were prepared at 50 µM in 500 µL of saturated 1-octanol in microfuge tubes, and then an equal volume of saturated water was added (total volume = 1 mL). The mixtures were shaken by hand exactly 200 times, centrifuged at 11,000 rpm for 2 minutes (BioRad Model 16K Microcentrifuge), and then separated and analyzed by absorption spectroscopy using a microplate reader (Molecular Devices SpectraMax M2^e). The concentrations of the C^N complexes in both layers were calculated from absorption standard curves constructed from known concentrations of the compounds. If no absorption was detected in a layer, the assumption that <0.1% of the initial concentration ended up in that layer was used for the log P calculation. Formally, log P_o/w is the log of the ratio of concentrations in the two layers.

The relative lipophilicities of the complexes were determined using a modified “shake flask” method as described in previous work (57 (link)). A saturated solution of 1-octanol with water was prepared by mixing 16 mL of 1-octanol (99.9 %) with 4 mL of deionized water (Milli-Q). A saturated solution of water with 1-octanol was prepared by mixing 16 mL of deionized water with 4 mL of 1-octanol. The saturated solutions were shaken for 24 h at ambient temperature using a New Brunswick Classic C25KC Incubator Shaker set at 230 rpm before further use. Starting with 500 μL of 50 μM complex (chloride salt) prepared in saturated 1-octanol, an equal volume of saturated water was added to give a total volume of 1 mL. The mixtures were shaken 200 times, centrifuged at 11,000 rpm (10,000 ×g) for 2 minutes using a BioRad Model 16K Microcentrifuge; the two resultant layers were then separated with a syringe. The concentrations of the complexes in both octanol and water layers were calculated from absorption measurements using a microplate reader (SpectraMax M2e) and standard curves in each saturated solvent.