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The TRH-GAL4 is a genetic tool used in Drosophila research. It is a transgenic fly line that expresses the GAL4 transcriptional activator under the control of the Thyrotropin-Releasing Hormone (TRH) promoter. This allows for the targeted expression of genes of interest in TRH-producing cells in the fly brain.

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2 protocols using trh gal4

1

Fly Genetics: Comprehensive Toolkit

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The following strains were used in this study: fru-GAL4 (Stockinger et al., 2005 (link)), UAS>stop>TNTin, UAS>stop>TNT (Stockinger et al., 2005 (link)), UAS>stop>mCD8::GFP (Yu et al., 2010 (link)), FLP from Liqun Luo (Department of Biology, Stanford University, CA, United States), tsh-GAL80 from Julie Simpson, TH-GAL4 from Serge Birman (Development Biology Institute of Marseille, Marseille, France), TRH-GAL4 (Alekseyenko et al., 2010 (link)), orco-GAL4, ppk23-GAL4, elavc155-GAL4, and the Canton-S strain from the Bloomington Stock Center, Bloomington, IN, United States.
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2

Diverse Drosophila Transgenic Lines

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The following lines were obtained from the Bloomington Drosophila Stock Center: nSyb-GAL4 (#51635), vGlut-GAL4 (#26160), ChAT-GAL4 (#6793), vGAT-GAL4 (#58409), Trh-GAL4 (#38389), Tdc2-GAL4 (#9313), OK107-GAL4 (#854), Akh-GAL4 (#25683), Mip-GAL4 (#51983), Ilp2-GAL4 (#37516), Eth-GAL4 (#51982), Fmrfa-GAL4 (#56837), Lk-GAL4 (#51993), NPF-GAL4 (#25681), phm-GAL4 (#80577), ple-GAL4 (#8848), SifAR-GAL4 (#76670), Tk-GAL4 (#51973), Eip93F RNAi (#57868), EcR RNAi (#58286), vGAT RNAi (#41958), and tubP-GAL80ts (#7019). E93-RNAi, nSyb-GAL4, mCherry-RNAi lines were outcrossed for five generations to a Berlin background (originally from the Heisenberg lab). Flies were raised on standard cornmeal/molasses food and incubated at 25 °C under a 12 h light/12 h dark cycle.
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