Alkaline phosphatase alp activity assay kit

Purified paeoniflorin and DEX (>98%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). They were dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich) to prepare their 100 mM stock solutions and were diluted in a cell culture medium for obtaining different interventional concentrations. Cell Counting Kit-8 (CCK-8) and Annexin V-FITC Apoptosis Detection Kit were obtained from Dojindo Molecular Technologies, Inc. (Rockville, MD, USA). Alkaline phosphatase (ALP) activity assay kit, ALP, and Alizarin Red staining reagents were obtained from Beyotime Institute of Biotechnology (Jiangsu, China). The phosphorylation-specific antibodies of AKT and mammalian target of rapamycin (mTOR), together with their pan-antibodies, were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). Runx2, Bcl-2, Bax, cleaved caspase-3, and total caspase-3 antibodies were purchased from Abcam (Cambridge, MA, USA) for use in western blot and immunochemistry. SC79, an AKT activator, was dissolved in DMSO and diluted with cell culture medium to 1 μM.