Klm 1

We purchased the human pancreatic cancer cell lines (PK-1, PK-8, KLM-1, Panc-1 and MIAPaca2) from RIKEN BioResouce Research Center (RIKEN BRC, Ibaraki, Japan) and another human pancreatic cancer cell line (BxPC-3) and Plat-A amphotropic retrovirus packaging cells from American Type Culture Collection (ATCC, Manassas, VA, USA). We maintained PK-1, PK-8, KLM-1, Panc-1 and BxPC-3 in RPMI-1640 (Nacalai Tesque) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, St. Louis, MO, USA), 100 U/ml penicillin (Life Technologies, Carlsbad, CA, USA) and 100 μg/ml streptomycin (Life Technologies) at 37°C in a humidified 5% CO₂ incubator. We maintained MIAPaca2 and Plat-A in DMEM (Nacalai Tesque) supplemented with 10% FBS, 100 U/ml penicillin and 100 μg/ml streptomycin. In Plat-A culture, 1 μg/ml of puromycin (Nacalai Tesque) and 10 μg/ml of blasticidin (Funakoshi, Tokyo, Japan) were added.

We purchased human PDAC cell lines (PK-1, PK-8, KLM-1, Panc-1 and MIAPaca2) from RIKEN BioResource Research Center (RIKEN BRC, Ibaraki, Japan) and another human PDAC cell line (BxPC-3) from American Type Culture Collection (ATCC, Manassas, VA, USA). We maintained PK-1, PK-8, KLM-1, Panc-1 and BxPC-3 cells in RPMI-1640 (Nacalai Tesque, Kyoto, Japan) supplemented with 10% fetal bovine serum (FBS) (Merck KGaA, Darmstadt, Germany, or Life Technologies, Carlsbad, CA, USA), 100 U/ml penicillin (Life Technologies) and 100 µg/ml streptomycin (Life Technologies). We maintained MIAPaca2 cells in DMEM (Nacalai Tesque) supplemented with 10% FBS, 50 U/ml penicillin and 50 µg/ml streptomycin. We maintained all cell lines at 37 °C in a humidified atmosphere containing 5% CO₂.