Anti nse

The antibodies used in the following experiments were: anti-Neu, anti-NSE, anti-nNOS, anti-eNOS anti-ICAM and anti-VE-Cadherin brought from Abcam (Cambridge, MA). Anti-Claudin-5 conjugated with Alexa Fluor 488 brought from Invitrogen (Carlsbad, CA). Anti-GAPDH from Millipore (Temecula, CA). Alexa Fluor 488-conjugated bovine serum albumin (BSA-Alexa Fluor-488) and secondary antibodies conjugated with Alexa fluor 488,Texas Red and 4’,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI) were purchased from Invitrogen (Carlsbad, CA). Radio-Immunoprecipitation Assay buffer was from Boston BioProducts (Worcester, MA); while Protease-Inhibitor Cocktail and BSA-Fluorescein Isothiocyanate (FITC) were from Sigma-Aldrich Chemicals (St Louis, MO). For western blot experiments. All buffers were prepared from synthesized 10X stock solutions.10X Running Buffer (60.4g Tris base, 288g glycine and 20g Sodium dodecyl-sulfate in 2L of sterile ddH₂ O), 10X Transfer Buffer (288g glycine, 60.4g Tris base, 1% SDS in 2L of sterile ddH₂ O) and 10x Tris Buffered Saline (TBS) (24.2g Tris base, 80g NaCl in 1L of sterile ddH₂ O; pH 7.4). Western blots were developed in Luminata Forte Western HRP Substrate (Millipore, Billerica, MA).