Milli q academic

1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC, MW 790), cholesterol (CHOL, ovine wool, MW 387), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (18:0 PEG2000 PE, MW 2805), and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG2000 amine, MW 2791) were purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA). GA (MW 170), holo-Tf human (MW 80,000), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC, MW 192), thioflavin T (ThT, MW 319), chloroform (MW 119), and 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP, MW 168) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Human amyloid-β peptide with 42 amino acid residues (Aβ_1-42, MW 4514) was acquired from GenScript Biotech (Piscataway, NJ, USA). Uranyl acetate dihydrate (MW 424) was purchased from Electron Microscopy Sciences (Hatfield, PA, USA). Diethyl ether (MW 74) was obtained from José Manuel Gomes dos Santos, Lda (Odivelas, Portugal), while methanol (MW 32) and ethanol absolute (MW 46) were bought from VWR International (Radnor, PA, USA). VWR International also provided phosphate-buffered saline (PBS) tablets (pH 7.4, 10 mM phosphate buffer, 2.7 mM potassium chloride, and 137 mM sodium chloride). PBS was prepared using ultrapure water (Milli-Q Academic, Millipore, Burlington, MA, USA).

The experiment was performed with the native and the most widespread strawberry variety (Sweet Charlie) from Zhengzhou during the harvest of 2019. The soluble solid content, total acidity, and pH of ripe strawberries at harvest were 10.8%, 10.7 g/L (as tartaric acid), and 3.03. In this work, the strawberries were harvested and collected in plastic cases of 2.5 kg capacity each.
Organic acid standards with purity > 99% were purchased from Dr. Ehrenstorfer GmbH Co. (Augsburg, Bavaria, Germany). Other chemical standards, including gallic acid; rutin; cyanidin-3-O-glucoside; ascorbic and 2-octanol; Folin–Ciocalteu reagent; 1, 1-diphenyl-2-picrylhydrazyl (DPPH); and 2, 2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) with purity > 98% were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). (NH₄)₂HPO₄ analytic chemicals were purchased from Shanghai Macklin Biochemical Co. (Shanghai, China). Water was purified using Milli-Q Academic (Millipore, Molsheim, France).

By microfluidic homogenization, we prepare a uniform silicone oil-in-water (O/W) microscale emulsion (poly-dimethyl siloxane oil, Gelest Inc.; viscosity: ${\nu }_{\text{o}}=350$ cSt), SDS (Fisher Scientific; electrophoresis grade 99% purity), and deionized water (Millipore Milli-Q Academic; resistivity: 18.2 M $\mathrm{\Omega }$ cm), as follows (see also Supplementary Information). We size-fractionate an initial polydisperse emulsion four times and repeatedly centrifuge to obtain a concentrated master ‘stock’ emulsion at fixed bulk [SDS] = 35 mM in a manner similar to Kim et al.^{47 (link)}. Dynamic and static light scattering measurements provide primary characteristics of the droplet radial size distribution: average hydrodynamic radius $⟨a⟩=484\pm 12$ nm and polydispersity $\delta a/⟨a⟩\simeq 0.15$ , where $\delta a$ is the standard deviation of this distribution. Using a gravimetric evaporation method^{63 (link)}, we determine that the oil droplet volume fraction of the master emulsion is ${\varphi }_{\text{m}}=0.793\pm 0.003$ . Emulsions at lower $\varphi$ are obtained by diluting this master emulsion with 35 mM SDS solution and stirring until uniform (see Supplementary Information).

Analytical pure samples of emtricitabine (C.C. Lab, Torrent Pharmaceutical Ltd., Indrad, India), tenofovir disoproxil fumarate (Sequent Research Lab., Mangalore, India), and rilpivirine (USV Ltd., Himachal Pradesh, India) were used in this study. The pharmaceutical dosage form used in this study was Complera procured from the local market and labelled to contain 200 mg of emtricitabine, 300 mg of tenofovir disoproxil fumarate, and 25 mg of rilpivirine per tablet. The solvent of acetonitrile (MeCN) was of HPLC grade and potassium dihydrogen phosphate, sodium hydroxide, hydrochloric acid, hydrogen peroxide, and triethylamine were of analytical reagent grade purchased from Merck, India. The HPLC grade water was prepared by using Milli-Q Academic, Millipore, Bangalore, India.

The cement samples were randomly allotted to one of four groups (n = 6) with different dimensions and condensed into cylindrical acrylic resin (Plexiglas) vessels. Obturation efficiency was verified by radiographs and weight. The four groups had varying length (L), diameter (ϕ), ESA and Vol are shown in Table 6.
The groups were designed in such a way that groups 1 and 2 had a smaller ESA compared to groups 3 and 4. The Vol increased from groups 1 to 4. Immediately after the initial setting time, each sample was separately immersed in 10 mL deionized ultra-pure water (MilliQ Academic, Millipore, Bedford, MA, USA) and gently shaken at 37 °C. After 3 h, 1, 3, 7, 14 and 28 days the samples were taken out of the solution, blotted dry with filter paper and transferred to 10 mL of fresh deionized water for further elution. pH and calcium content of the solutions were measured.

The SS (304L, Equinox, Willems, France) and PC (Plexilux, Vaux-le-Pénil, France) slides were soaked in ethanol 95° (Fluka, Sigma-Aldrich, Saint-Quentin-Fallavier, France) for an overnight and then rinsed twice with distilled water. Then the slides were soaked in 500 ml of DDM ECO detergent (1%) for 15 min at room temperature (20 °C) under agitation (ANIOS, Villeneuve d’Ascq, France). Slides were thoroughly rinsed five times, for one min under agitation, in 500 ml of distilled water and three times in ultrapure water (Milli-Q^® Academic, Millipore, Guyancourt, France) at 20 °C to eliminate detergent residues. SS slides were air-dried and sterilized by autoclaving at 121 °C for 20 min. PC slides were sterilized in the ethanol 95° for 15 min.

Stainless steel (SS) slides (304L, Equinox, France) of 41 mm diameter and 1 mm thickness were used for this study. Slides were immersed overnight in 95% ethanol (Fluka, Sigma-Aldrich, France), then washed with distilled water. The rinsed slides were immersed again in 1% DDM ECO detergent (ANIOS, France) for 15 min at 20°C. The slides were afterwards washed vigorously with distilled water 5 times for 1 min, followed by three ultra-pure water washes (Milli-Q® Academic, Millipore, France) to completely remove detergent residues. Finally, SS slides were air-dried before sterilization by autoclaving at 121°C for 20 min. The sterile slides were placed in a static biofilm reactor, called NEC biofilm System, for biofilm deposition. This system as previously described by Abdallah et al. [25 (link)], is composed of several assembled SS parts and a rubber O-ring. Briefly, the SS lower part forms the circular base of the system, and the O-ring placed on the upper plate side is used to tightly match the SS slide. The SS cylinder with two holes is used to form a well for biofilm formation with oxygen supply. The clamp is used for sealing and the metal cover is used to maintain the sterility of the closed system. All parts were autoclaved at 121°C for 20 min.