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Ammonium persulfate

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Ammonium persulfate is a white crystalline compound used as an oxidizing agent, initiator, and catalyst in various applications. It is a commonly used reagent in biochemical and analytical laboratory procedures.

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Lab products found in correlation

Acrylamide, by Avantor (3 mentions) Sodium dodecyl sulfate (sds), by Avantor (3 mentions) N n n n tetramethylethylene diamine, by Avantor (3 mentions) Methylene bisacrylamide, by Avantor (2 mentions) Bromophenol blue, by Bio-Rad (2 mentions) Sucrose, by Avantor (2 mentions) Glycerol, by Avantor (2 mentions) Mannitol, by Avantor (2 mentions) Glycine, by Avantor (2 mentions) Immobilized ph gradient strips, by Bio-Rad (2 mentions) Chaps, by Bio-Rad (2 mentions) Trypsin, by Promega (2 mentions) Iodoacetamide, by Bio-Rad (2 mentions) Trichloroacetic acid, by Avantor (2 mentions) Acrylamide, by Bio-Rad (2 mentions) Tris base, by Avantor (1 mentions) Tween 20, by Avantor (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Merck Group (1 mentions) Pvdf membrane, by Merck Group (1 mentions) β actin, by Cell Signaling Technology (1 mentions)

15 protocols using ammonium persulfate

1

Immunoblotting Analysis of Autophagy and Apoptosis

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Metformin, PMSF, leupeptin and aprotinin were purchased from Sigma (St. Louis, MO, USA). Acrylamide, methylene bisAcrylamide, sodium dodecyl sulfonate (SDS), Tris base, ammonium persulfate and Tween-20 were obtained from Amresco (Solon, OH, USA). Fetal bovine serum and DMEM were purchased from HyClone (Logan, UT, USA). Antibodies against p62, phospho-AMPKα (Thr172), phospho-mTOR (Ser2448), mTOR, phospho-p70 S6 Kinase (Thr421/Ser424), p70 S6 Kinase and β-actin were obtained from Cell Signaling (Danvers, MA, USA). Antibodies against PARP1, Caspase-9 and Caspase-3 were purchased from Abcam (Cambridge, MA, USA). Antibody against microtubule-associated protein 1 light chain 3 II (LC3-II) was from GeneTex (Irvine, CA, USA). Anti-rabbit/mouse goat immunoglobulin G-horseradish peroxidase (HRP) secondary antibodies were obtained from Santa Cruz (Santa Cruz, CA, USA). PVDF membrane was purchased from Millipore (Billerica, MA, USA). ECL Plus immunoblotting detection kit was from Bio-Rad (Hercules, CA, USA). Other routine reagents were obtained from Zhongshan (Beijing, China).
Gao C., Fang L., Zhang H., Zhang W.S., Li X.O, & Du S.Y. (2020). Metformin Induces Autophagy via the AMPK-mTOR Signaling Pathway in Human Hepatocellular Carcinoma Cells. Cancer Management and Research, 12, 5803-5811.
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2

Proteomic Analysis of Mitochondrial Dysfunction

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Acetonitrile, trifluoroacetic acid (TFA), K3Fe(CN)6, HCCA, 5-hydroxydecanote (5-HD), Nycodenz and urea were purchased from Sigma (St. Louis, MO, USA). Bromophenol blue, acrylamide, CHAPS, immobilized pH gradient (IPG) strips, two-dimensional polyacrylamide gel electrophoresis (2DE) Quant kit, 2DE Clean-up Kit, polyvinylidene fluoride membrane and low melting point agarose were purchased from Bio-Rad (Hercules, CA, USA). Glycerol, glycine, ammonium persulfate, N, N′-methylene acrylamide, SDS, Tris, EDTA, sucrose and mannitol were supplied by Amresco (Solon, OH, USA). Secondary antibodies and anti-GRP75, anti-NDUFV1, anti-DLD and anti-cytochrome oxidase IV (COX IV) primary antibodies were obtained from Abcam (Cambridge, UK). TEMED was from Amersham (UK). Butanol was from Merck (Darmstadt, Germany). Trypsin was from Promega (Madison, WI, USA). Pentobarbital sodium and Tween 20 were both from Solarbio (Beijing, China). All other unlisted reagents were of analytical grade or guaranteed reagents.
Cao S., Liu Y., Wang H., Mao X., Chen J., Liu J., Xia Z., Zhang L., Liu X, & Yu T. (2016). Ischemic postconditioning influences electron transport chain protein turnover in Langendorff-perfused rat hearts. PeerJ, 4, e1706.
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3

Immunoblot Analysis of Mitochondrial Proteins

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SDS, ammonium persulfate, sucrose, acrylamide, methylene bis-acrylamide, mannitol, glycerol and glycine were purchased from Amresco, LLC. Diazoxide, Nycodenz®, urea, thiourea, EDTA and tetramethylethylenediamine were obtained from Sigma-Aldrich (Merck KGaA). Protein quantification kits, immobilized pH gradient (IPG) strips, dithiothreitol (DTT), BIO-Lyte, CHAPS, agarose, bromophenol blue, β-mercaptoethanol, iodoacetamide and PVDF were acquired from Bio-Rad Laboratories, Inc. Anti-cytochrome c oxidase subunit IV (COX IV; cat. no. ab14744), anti-2-oxoglutarate dehydrogenase (OGDH; cat. no. ab137773), anti-NADH dehydrogenase (ubiquinone) flavoprotein 1 (NDUFV1; cat. no. ab203208) and anti-NADH-ubiquinone oxidoreductase 75 kDa subunit (NDUFS1; cat. no. ab169540) antibodies were purchased from Abcam. All reagents were of analytical grade.
Pan Y., Wang Y., Shi W., Liu Y., Cao S, & Yu T. (2020). Mitochondrial proteomics alterations in rat hearts following ischemia/reperfusion and diazoxide post-conditioning. Molecular Medicine Reports, 23(2), 161.
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4

Tris-based Protein Extraction Protocol

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Tris, octylphenoxypolyethoxyethanol (NP-40), urea, sulfourea, 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), sodium dodecyl sulphate (SDS), acrylamide, N,N′-methylenebisacrylamide, ammonium persulfate, N,N,N′,N′-tetramethylethylene diamine, CBB G-250 and trichloroacetic acid were obtained from Amresco (Solon, OH, USA); phenylmethylsulfonyl fluoride was obtained from Sigma (St. Louis, MO, USA); immobilized pH gradient strips (IPG strips, 17 cm, pH 4–7) and iodoacetamide were obtained from Bio-Rad Laboratories (Hercules, CA, USA); acetone, glycerol, phosphoric acid, carbinol and alcohol (analytical reagents) were obtained from manufacturers in China. All water used in this experiment was Milli-Q hyperpure water (Millipore, Billerica, MA, USA).
Lu N., Xu Z., Meng B., Sun Y., Zhang J., Wang S, & Li Y. (2014). Proteomic Analysis of Etiolated Juvenile Tetraploid Robinia pseudoacacia Branches during Different Cutting Periods. International Journal of Molecular Sciences, 15(4), 6674-6688.
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5

Protein Sample Preparation for Mass Spectrometry

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Urea, DL-dithiothreitol (DTT), iodoacetamide (IA), IPG buffer and formic acid (FA) were purchased from GE Healthcare. SDS, Tris-(hydroxymethyl)-amino-methane, trichloroacetic acid, ammonium persulfate and N,N,N',N'-tetramethylethylenediamine were purchased from Amresco. Tetraethyl-ammonium bromide (TEAB) and Coomassie brilliant blue G-250 were obtained from Sigma-Aldrich (Merck KGaA). Trypsin was from Promega Corp. and acetonitrile (ACN; HPLC grade) and H2O were purchased from Thermo Fisher Scientific, Inc.
Wang D., Yu H., Li Y., Xu Z., Shi S., Dou D., Sun L., Zheng Z., Shi X., Deng X, & Zhong X. (2021). iTRAQ-based quantitative proteomics analysis of the hepatoprotective effect of melatonin on ANIT-induced cholestasis in rats. Experimental and Therapeutic Medicine, 22(3), 1014.
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6

Serum Proteome Analysis Workflow

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Reagents. Urea was purchased from Gibco; Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Propanesulfonic acid (CHAPS), ethylenediaminetetraacetic acid (EDTA), and dithiothreitol (DTT) were purchased from Promega Corporation (Madison, WI, USA). Iodoacetamide (IAM) was purchased from Promega Corporation, and sodium dodecyl sulfate (SDS), bromophenol blue, and a serum high-abundance protein depletion kit (ProteoPrep Blue Albumin and IgG Depletion kit) were purchased from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany). Ammonium persulfate was purchased from Amresco, LLC (Solon, OH, USA), and acetonitrile (MS grade) was purchased from Thermo Fisher Scientific, Inc.
Collection and preparation of serum. A total of 5 ml of blood was collected from the elbow vein of patients during the early morning under conditions of limosis. The samples were incubated at 4˚C for 2 h, centrifuged at 4˚C and 3,000 x g for 10 min to separate the serum after self-coagulation, and then stored at -80˚C. Depletion of high-abundance proteins was carried out using a serum high-abundance protein depletion kit according to the manufacturer's instructions.
Li Y.L., Qi R.Q., Yang Y., Wang H.X., Jiang H.H., Li Z.X., Xiao B.H., Zheng S., Hong Y.X., Li J.H., Chen H.D, & Gao X.H. (2018). Screening and identification of differentially expressed serum proteins in patients with vitiligo using two‑dimensional gel electrophoresis coupled with mass spectrometry. Molecular medicine reports, 17(2).
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7

Graphene Transfer to Alternate Substrate

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To transfer graphene to another substrate, one side of the as-synthesized SLG was coated with PMMA (Microchem) resist and cured at 180°C for 1 min. The other side of the sample was exposed to O2 plasma to remove the graphene on that side. The sample was then left in Ammonium persulfate (VWR, 98%) solution for overnight to completely dissolve away the copper layer. Then the graphene was transferred to one side of a Cu foil with almost the same size. The process was repeated to transfer another piece of graphene to the other side of the Cu foil. The PMMA coating was removed with acetone and isopropanol. Finally, the Cu foil was rinsed with deionized water several times.
Zhang W., Lee S., McNear K.L., Chung T.F., Lee S., Lee K., Crist S.A., Ratliff T.L., Zhong Z., Chen Y.P, & Yang C. (2014). Use of graphene as protection film in biological environments. Scientific Reports, 4, 4097.
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8

Modulation of Extracellular Matrix Stiffness

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Poly-acrylamide (PA) gels of varying bisacrylamide concentrations were created on amino-silanated coverslips as previously described [68] . PA gel solutions were produced by combining acrylamide and bisacrylamide to final concentrations of 8% acrylamide (Biorad, Hercules, CA, USA) and 0.048%, 0.117%, 0.208%, or 0.260% bisacrylamide (Biorad) to obtain gels with final elastic moduli of 2 kPa, 8 kPa, 16 kPa, or 24 kPa, respectively. Fifty (50) µl of each solution was polymerized by the addition of ammonium persulfate (VWR, West Chester, PA, USA) and N,N,N′,N′-tetramethylethylenediamine (Biorad, Hercules, CA, USA) (1% and 0.1% final concentration respectively). The gels were allowed to polymerize for approximately 30 minutes, then washed three times with PBS. Fn was covalently attached to the surface using the heterobifunctional crosslinker sulfosuccinimidyl-6- (4′-azido-2′ nitrophenyl-amino) hexanoate (sulfo-SANPAH; Pierce Chemical Co., Rockford, IL., USA). Following an overnight incubation with the Fn, gels were washed three times with PBS.
Dysart M.M., Galvis B.R., Russell A.G, & Barker T.H. (2014). Environmental Particulate (PM2.5) Augments Stiffness-Induced Alveolar Epithelial Cell Mechanoactivation of Transforming Growth Factor Beta. PLoS ONE, 9(9), e106821.
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9

Synthesis and Characterization of Polymeric Materials

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l-Lactide (LA, Serva Feinbiochemica) was recrystallized from toluene twice, polyethylene glycol monomethyl ether (mPEG2k, Sigma-Aldrich) was dried under reduced pressure, and stannous 2-ethylhexanoate (Sn(Oct)2, ∼95%, Sigma-Aldrich) was dried over molecular sieves. Dicyclohexylcarbodiimide (DCC), 4-(dimethylamino)pyridine (DMAP), ammonium hydroxide, hydrochloric acid (37%), succinic anhydride, p-phenylenediamine, N-phenyl-p-phenylenediamine, and phenyl hydrazine (97%) were all obtained from Sigma-Aldrich and used without further purification; ammonium persulfate, ethanol, and dichloromethane were obtained from VWR. Dimethylformamide (DMF, HPLC, VWR) was dried over CaH2 and distilled under reduced pressure before use. Methanol (MeOH, HPLC, Fisher Scientific), trichloroacetyl isocyanate (96%, Sigma-Aldrich), 1,6-diphenyl-1,3,5-hexatriene (DPH, 98%, Sigma-Aldrich), chloroform (99%, Fisher Scientific), diethyl ether (99.8%, Sigma-Aldrich), anhydrous tetrahydrofuran (THF, Sigma-Aldrich), and hexamethylene diisocyanate (HMDI, 99%, Sigma-Aldrich) were all used without further purification.
Glavas L., Odelius K, & Albertsson A.C. (2014). Induced redox responsiveness and electroactivity for altering the properties of micelles without external stimuli †Electronic supplementary information (ESI) available. See DOI: 10.1039/c4sm00258j Click here for additional data file.. Soft Matter, 10(22), 3859-3860.
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10

Traction Stress Measurement on Hydrogels

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Cell tractions were measured as described and calculated using a custom Matlab routine (27 (link)). 2% v/v of 0.2 μm diameter 580/605 FluoSpheres microspheres (Invitrogen) were added to the prepolymer solution, comprised of 5% acrylamide, 0.06% bis-acrylamide, 1% ammonium persulfate (Fisher), and 0.1% v/v of N,N,N’,N’-Tetramethylethylenediamine (VWR International). Gels were prepared in 12 well glass bottom plates (Cellvis), which were precleaned in a UV/Ozone cleaner (ProCleaner™ Plus, Bioforce Nanosciences) and methacrylated to ensure binding of the gel. Collagen was bound to the surface by adding 0.2 mg/ml sulfo-SANPAH and activating with UV light (wavelength 350 nm) for 10 minutes followed by incubation with 0.15 mg/ml type I collagen. Isolated cells were seeded at ~15,000 cells/cm2 on the gels and allowed to adhere for 3 hrs. Brightfield images were taken of each cell prior to obtaining microsphere displacements at 60x. Bead reference positions were then re-obtained after removing the cells with a 10% v/v Triton X solution for 10 minutes. Strain energy was determined from the traction stress map and normalized to cell area.
Beri P., Popravko A., Yeoman B., Kumar A., Chen K., Hodzic E., Chiang A., Banisadr A., Placone J.K., Carter H., Fraley S.I., Katira P, & Engler A.J. (2019). Cell adhesiveness serves as a biophysical marker for metastatic potential. Cancer research, 80(4), 901-911.
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