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2 protocols using nbp1 31333

1

Antibody Panel for Adipose Protein Analysis

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The protease inhibitor cocktail (cOmplete Mini) and a phosphatase inhibitor cocktail (PhosSTOP) were obtained from Roche Diagnostics. The following antibodies were obtained from Cell Signaling Technology: Cox4 (4844), PPARγ (2435), C/EBPα (8178), p-Ser239 VASP (3114), total VASP (3112), p-Ser473 AKT (4060), p-Thr308 AKT (9275), total AKT (9272), p-Ser563 HSL (4139), total HSL (4107), ATGL (2439), ADIPOQ (2789), GLUT4 (2213), ACC (3676), FASN (3180), and β-actin (4967). Other antibodies used include NPRC (NBP1–31365, Novus Biologicals), NPRA (NBP1–31333, Novus Biologicals), NDUFS4 (PA5–21677, Pierce Biotechnology), UCP1 (ab23841, Abcam), and GAPDH (sc25788, Santa Cruz Biotechnology) and CYTOC (sc13156, Santa Cruz Biotechnology). Secondary antibodies against rabbit immunoglobulin G (IgG) (A3687) and mouse IgG (A3562), both conjugated with alkaline phosphatase, were from Sigma-Aldrich.
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2

Antibody Panel for Adipose Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The protease inhibitor cocktail (cOmplete Mini) and a phosphatase inhibitor cocktail (PhosSTOP) were obtained from Roche Diagnostics. The following antibodies were obtained from Cell Signaling Technology: Cox4 (4844), PPARγ (2435), C/EBPα (8178), p-Ser239 VASP (3114), total VASP (3112), p-Ser473 AKT (4060), p-Thr308 AKT (9275), total AKT (9272), p-Ser563 HSL (4139), total HSL (4107), ATGL (2439), ADIPOQ (2789), GLUT4 (2213), ACC (3676), FASN (3180), and β-actin (4967). Other antibodies used include NPRC (NBP1–31365, Novus Biologicals), NPRA (NBP1–31333, Novus Biologicals), NDUFS4 (PA5–21677, Pierce Biotechnology), UCP1 (ab23841, Abcam), and GAPDH (sc25788, Santa Cruz Biotechnology) and CYTOC (sc13156, Santa Cruz Biotechnology). Secondary antibodies against rabbit immunoglobulin G (IgG) (A3687) and mouse IgG (A3562), both conjugated with alkaline phosphatase, were from Sigma-Aldrich.
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