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Uric acid

Manufactured by Randox
Sourced in United Kingdom

Uric acid is a laboratory test used to measure the level of uric acid in the blood. Uric acid is a waste product that is produced when the body breaks down purines, which are found in certain foods and are also produced naturally by the body. The uric acid test is used to help diagnose and monitor conditions such as gout, kidney stones, and other disorders related to uric acid metabolism.

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4 protocols using uric acid

1

Biochemical Assays and Cytokine Quantification

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RES raw powders were purchased from Sigma Chemical Co. (St. Louis, MO, USA), while ISO injection was obtained from a local pharmacy. L-RES was purchased from Lipolife, UK. L-RES has a particle size less than 200 nm with a neutral zeta potential as stated by the manufacturer. Urea, creatinine and uric acid biochemical assays were supplied from Randox, Crumlin, UK. IL-6 ELISA kit was obtained from R&D Systems (Minneapolis, MN, USA). MAPK, cystatin c and β-actin antibodies were purchased from Novus Biologicals (Centennial, CO, USA). The PCR primers were obtained from Sigma (St. Louis, MO, USA).
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2

Sesamin-Cyclophosphamide Nephroprotective Assay

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Sesamin, cyclophosphamide, 5,5dithiobis-(2-nitrobenzoic acid), reduced GSH, TBA, were obtained from Sigma chemicals (3300 S 2nd St #3306 St. Louis, MO 63118, USA). Biochemical assay kits for BUN, Uric Acid, and Creatinine were obtained from Randox, Crumlin, UK. Elisa estimation kits for IL-1β, TNFα, and Caspase-3 were purchased from Abcam, Cambridge, UK.
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3

Cytokine and enzyme assays in UA/CP model

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UA (CAS No. 77.52-1) and CP (CAS No. 15,663-27-1) were purchased from Sigma Aldrich (USA). ELISA kits such as IL-1β (CAT No. ab100768), IL-6 (CAT No. ab234570), and TNF-α (CAT No. ab46070); colorimetric kits for caspase-3 (CAT No. ab39401) and caspase-9 (CAT No. ab65608) were purchased from Abcam, UK. Biochemical kits such as Cr, BUN, and uric acid were obtained from Randox, UK. All other chemicals and solvents used during the experiment were laboratory grade and purchased from commercial sources.
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4

Biochemical Diagnostics Using Commercial Kits

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Urea, uric acid, creatinine, and enzyme diagnostic kits (ALT, AST, ALP and ACP) were obtained from Randox Laboratories Limited, United Kingdom. Sodium benzoate salt is a product of May and Baker Ltd. Dagenham, England. All other chemicals which are products of Sigma Chemical Company, St Louis, USA were of analytical grade and were prepared in the laboratory in double glass distilled water.
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