Dna polymerase and restriction enzymes

Manufactured by Takara Bio

DNA polymerase is an enzyme that catalyzes the synthesis of DNA molecules by adding nucleotides to a DNA strand. Restriction enzymes are enzymes that recognize and cleave specific DNA sequences, allowing for the manipulation of DNA fragments.

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PrimeSTAR HS DNA polymerase and restriction enzymes DNA restriction enzymes Restriction enzymes DNA polymerase

2 protocols using dna polymerase and restriction enzymes

Porcine Mitochondrial Malate Dehydrogenase Purification

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Mitochondrial malate dehydrogenase (MDH) from porcine was purchased from Sigma. T1 lipase was expressed and purified as previously described [21] (link). DNA polymerase and restriction enzymes were purchased from Takara.

Liu L., Chen J., Yang B, & Wang Y. (2015). Oligomer-dependent and -independent chaperone activity of sHsps in different stressed conditions. FEBS Open Bio, 5, 155-162.

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Streptomyces DNA Manipulation Protocol

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Streptomyces sp.MSC090213JE08 chromosome DNA was used as a template for PCR unless otherwise specified. E. coli strains JM109 and BL21(DE3) were used as hosts for DNA manipulation and recombinant protein production, respectively. BD Difco LB medium and TB medium were used. TB medium (pH 7.2) contains Bacto tryptone (1.2%), Bacto yeast extract (2.4%), glycerol (0.8%), K₂HPO₄ (0.94%) and KH₂PO₄ (0.22%). The enzymes used for DNA manipulations, including DNA polymerase and restriction enzymes, were purchased from Takara Bio. Primers (Supplementary Table 3) were purchased from ThermoFisher Scientific.

Du D., Katsuyama Y., Horiuchi M., Fushinobu S., Chen A., Davis T.D., Burkart M.D, & Ohnishi Y. (2020). Structural basis for selectivity in a highly reducing type II polyketide synthase. Nature chemical biology, 16(7), 776-782.

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