Parp 1 sc 8007

Manufactured by Santa Cruz Biotechnology

Sourced in United States

PARP-1 (sc-8007) is a lab product offered by Santa Cruz Biotechnology. It is a protein that plays a role in cellular processes such as DNA repair, programmed cell death, and transcriptional regulation. The product is intended for research use only, and a detailed description of its core function can be provided without interpretation or extrapolation.

Automatically generated - may contain errors

Lab products found in correlation

Las 3000 imager, by Fujifilm (2 mentions) Ripa buffer, by Thermo Fisher Scientific (2 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Goat anti mouse igg hrp, by Santa Cruz Biotechnology (1 mentions) Goat anti rabbit igg hrp, by Santa Cruz Biotechnology (1 mentions) Polyvinylidene fluoride membrane, by Roche (1 mentions) Ecl kit, by Cytiva (1 mentions) Ripa buffer, by Boston BioProducts (1 mentions) β actin, by Merck Group (1 mentions) Protease and phosphatase inhibitor, by Roche (1 mentions) Cd133, by Cell Signaling Technology (1 mentions) Protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) Pakt t308, by Cell Signaling Technology (1 mentions) P gsk3β s9, by Cell Signaling Technology (1 mentions) Cdca8, by Abcam (1 mentions) Caspase 9, by Cell Signaling Technology (1 mentions) Flag tag (flag), by Merck Group (1 mentions) β catenin, by Cell Signaling Technology (1 mentions) Pvdf membrane, by Roche (1 mentions) Caspase 3, by Cell Signaling Technology (1 mentions)

4 protocols using parp 1 sc 8007

Protein Expression Profiling by Western Blot

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Equal amounts of total protein were fractionated by sodium dodecyl sulfate polyacrylamide gel electrophoresis on a 10% gel and transferred to a polyvinylidene fluoride membrane (Roche). The membranes were blocked with 5% milk/Tris-buffered saline plus Tween 20 (TBST) and incubated with primary antibodies against PARP-1 (sc-8007), NEK2 (sc-55601), β-actin (sc-47778) (all from Santa Cruz Biotechnology, Santa Cruz, CA, USA), pro-caspase-3 (#9662), β-catenin (#9562), phospho-ERK (#9101), ERK (#9102) (all from Cell Signaling, Danvers, MA, USA), and RPL17 (ab155781, Abcam, Cambridge, UK). HRP goat anti-mouse IgG, HRP goat anti-rabbit IgG, and HRP rabbit anti-goat IgG (Santa Cruz) were used as secondary antibodies. Immunoreactive bands were visualized using the LAS-3000 Imager (Fujifilm Corporation, Tokyo, Japan).

Ko M.J., Seo Y.R., Seo D., Park S.Y., Seo J.H., Jeon E.H., Kim S.W., Park K.U., Koo D.B., Kim S., Bae J.H., Song D.K., Cho C.H., Kim K.S, & Lee Y.H. (2022). RPL17 Promotes Colorectal Cancer Proliferation and Stemness through ERK and NEK2/β-catenin Signaling Pathways. Journal of Cancer, 13(8), 2570-2583.

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Western Blot Analysis of DNA Repair Proteins

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Cells were lysed in radioimmunoprecipitation (RIPA) buffer (Boston
Bioproducts) with a cocktail of protease and phosphatase inhibitors (Roche).
Protein (10–15 ug) was separated by 4–20% SDS-PAGE and transferred
to polyvinylidene difluoride membranes by electroblotting. After blocking with
5% non-fat dry milk in TBS-T (20 mM Tris [pH,7.5] 150mM NaCl, 0.1% Tween20) for
1–2 hours at room temperature, membranes were incubated with primary
antibody at 4°C overnight. Membranes were washed in TBS-T and incubated
with appropriate peroxidase conjugated secondary antibodies for 1 hour at room
temperature. Signals were visualized using the enhanced chemiluminescense (ECL)
kit (Amersham Bioscience). Primary antibodies used were: MSH6 (#5425), XRCC1
(#2735) (Cell Signal Technology), PARP1 (sc-8007)(Santa Cruz) and β-actin
(A1978)(Sigma).

Higuchi F., Nagashima H., Ning J., Koerner M.V., Wakimoto H, & Cahill D.P. (2020). Restoration of Temozolomide Sensitivity by Poly(ADP-Ribose) Polymerase inhibitors in Mismatch Repair Deficient Glioblastoma is Independent of Base Excision Repair. Clinical cancer research : an official journal of the American Association for Cancer Research, 26(7), 1690-1699.

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Protein Expression Analysis of siRNA Transfected Cells

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After 48 h of siRNA transfection, cells were lysed in RIPA buffer (Thermo Scientific, Rockford, IL, USA) containing 0.01% of protease and phosphatase inhibitor cocktail (Thermo Scientific). The protein concentration was measured with a Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL, USA). The cell protein extracts (50 μg) were separated on SDS-PAGE in 10% gel and transferred to PVDF membranes (Roche, Basel, Switzerland). After blocking with 5% skim milk/Tris-buffered saline plus Tween 20 (TBST), the membranes were incubated with primary antibodies against human ATF-3 (sc-188), GADD34 (sc-8327), p-Cdc2 (sc-12341), PARP-1 (sc-8007), β-actin (sc-4778), GSK-3β (sc-81462) (all from Santa Cruz Biotechnology, Santa Cruz, CA, USA), Cyclin B1 (ab72), Bax (ab32503), Akt (ab126811), CDCA8 (ab70910, Abcam, Cambridge, UK), p-Akt(T308) (#13038), p-GSK-3β(S9) (#9336), β-catenin (#9562), Caspase-9 (#9502), CD133 (#64326, Cell Signaling Technology, Danvers, MA, USA), and Flag (F1804, Sigma Aldrich, Saint Louis, MO, USA). HRP goat antimouse IgG and HRP goat antirabbit IgG (Santa Cruz) were used as the secondary antibodies. The blot signals were visualized with a LAS-3000 Imager (Fujifilm Corporation, Tokyo, Japan). All the uncropped Western blots are shown in Figures S5–S10.

Jeon T., Ko M.J., Seo Y.R., Jung S.J., Seo D., Park S.Y., Park K.U., Kim K.S., Kim M., Seo J.H., Park I.C., Kim M.J., Bae J.H., Song D.K., Cho C.H., Lee J.H, & Lee Y.H. (2021). Silencing CDCA8 Suppresses Hepatocellular Carcinoma Growth and Stemness via Restoration of ATF3 Tumor Suppressor and Inactivation of AKT/β–Catenin Signaling. Cancers, 13(5), 1055.

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Protein Expression Analysis in Rat Striatum

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Proteins were extracted from the rat striatum as described previously (Wang et al., 2017 (link)). The proteins were separated by SDS-polyacrylamide gel electrophoresis (PAGE). After electrophoresis, the proteins were transferred to polyvinylidine difluoride membranes. The membranes were then blocked with 5% milk-Tris-buffered solution-Tween solution for 2 h and subsequently incubated overnight at 4°C followed by appropriate secondary antibodies for 2 h at room temperature. Bands were visualized using the ECL system (BIO-RAD Laboratories, Inc., California, USA). Primary antibodies against toll-like receptor 4 (TLR4, sc-293072, Santa Cruz Biotechnology), myeloid differentiation factor 88 (MyD88, sc-74532, Santa Cruz), tumor necrosis factor (TNF) receptor associated factor 6 (TRAF6, sc-8409, Santa Cruz), nuclear factor (NF) κB (sc-8008, Santa Cruz), interleukin (IL)-1β (sc-12742, Santa Cruz), IL-6 (sc-57315, Santa Cruz), TNF-α (sc-12744, Santa Cruz), caspase 3 (9665, Cell Signaling Technology), poly(ADP-ribose) polymerase-1 (PARP-1, sc-8007, Santa Cruz), and GAPDH (sc-32233, Santa Cruz) were used.
Densitometric analysis was conducted using Tanon Gel Image System (version 4.2). Data of relative integrated optical density values of bands are presented as bar charts.

Xie X.L., Zhou W.T., Zhang K.K., Chen L.J, & Wang Q. (2018). METH-Induced Neurotoxicity Is Alleviated by Lactulose Pretreatment Through Suppressing Oxidative Stress and Neuroinflammation in Rat Striatum. Frontiers in Neuroscience, 12, 802.

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