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Dp 70 color camera

Manufactured by Olympus
Sourced in Japan

The Olympus DP-70 is a high-performance color camera designed for use in various laboratory applications. It features a high-resolution CCD sensor and advanced imaging capabilities, providing accurate and detailed color reproduction. The DP-70 is capable of capturing high-quality digital images, making it a reliable tool for microscopy, documentation, and other scientific imaging tasks.

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5 protocols using dp 70 color camera

1

Retinal Cell Density Quantification

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The prepared 5 μm-thick tissue sections were brought to room temperature and deparaffinized. The tissue sections were then stained with hematoxylin (Bio Lab Diagnostics, Mumbai, India) for 15 minutes and counterstained with 1% eosin (Bio Lab Diagnostics) for another 15 minutes. After dehydration by treating with ethanol at 50% concentrations for 2 minutes, 65% for 2 minutes, 85% for 2 minutes, 90% for 2 minutes, 100% for 10 minutes, xylene: ethanol (1:1) for 6 minutes and xylene for 6 minutes, sections were observed at 400× magnification under a light microscope (Olympus BX51TF microscope with DP70 color camera, Olympus, Tokyo, Japan). The cell density of the retina was evaluated by counting the number of neuronal cells per 0.1 mm2 area using the Cell Counter plugin of ImageJ v1.47 (Rasband, W.S., National Institutes of Health, Bethesda, MD, USA).
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2

Histological Analysis of Mouse Liver Fibrosis

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4% paraformaldehyde-fixed livers were embedded in paraffin at the Johns Hopkins School of Medicine Pathology Core. Paraffin sections were cut, and hematoxylin and eosin stained at the Pathology Core. To analyze fibrosis, paraffin sections were stained using a Picro Sirius Red Stain Kit (ab150681, Abcam) according to the manufacturer’s instructions. To immunostain CK19, Ki67, and active caspase-3, paraffin sections were deparaffinized, subjected to antigen retrieval with 1 mM EDTA using a pressure cooker for 15 min, and incubated with the primary antibody at 4°C overnight. After washes with PBS, the samples were incubated with HRP-conjugated secondary antibody for 1 h at room temperature and stained with DAB (ab64238, Abcam). Hematoxylin (MHS16, Sigma-Aldrich) was used to counterstain nuclei. The samples were viewed using an Olympus BX51 microscope equipped with a DP-70 color camera.
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3

Cryogenic Tissue Sectioning and Imaging

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After euthanasia, organs removed from CFZ and vehicle fed mice were cryo-preserved in optimal cutting temperature compound (Tissue-Tek 4583; Sakura). Tissue blocks were sectioned at a thickness 10 μm, using a Leica 3050S cryostat. For transmitted microscopy, cryo-sectioned slices were mounted on glass slides with glycerol, and imaged with Olympus X51 upright microscope equipped with X100 objective, DP-70 color camera, and DP controller 3.1.1267.
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4

Immunohistochemistry and Histology Protocol

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For immunohistochemistry, cryosections were stained with antibodies to B220 (1:100, Invitrogen; 14–0452-82) and CD3 (1:100, Sigma; SAB5500058). After washes with PBS, the samples were incubated with HRP-conjugated secondary antibody for 1 h at room temperature and stained with 3,3'-diaminobenzidine. For histology, paraformaldehyde-fixed tissues were embedded in paraffin at the Johns Hopkins School of Medicine Pathology Core. Paraffin sections were cut, and stained with hematoxylin and eosin. The samples were viewed using an Olympus BX51 microscope equipped with a DP-70 color camera.
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5

Epifluorescence Microscope Image Quantification

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Stained sections were imaged using BX51 epifluorescence microscope with DP70 color camera and CellSens software version 2.2 (Olympus, Tokyo, Japan) or the AxioScan.Z1 slide scanner with ZEN 2.6 software blue edition (Carl Zeiss, Oberkochen, Germany). Images were then quantified using FIJI ImageJ software version 1.52p (NIH, Bethesda, MD; https://imagej.nih.gov/ij). 15
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