Fei tecnai f20 tem

Manufactured by Thermo Fisher Scientific

Sourced in Japan

The FEI Tecnai F20 TEM is a high-performance transmission electron microscope (TEM) designed for advanced materials characterization. It features a field emission gun (FEG) source, providing high-brightness and high-resolution imaging capabilities. The Tecnai F20 TEM is capable of resolving fine structural details at the atomic scale.

Automatically generated - may contain errors

Lab products found in correlation

Tecnai g spirit biotwin transmission electron microscope, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

FEI TECNAI G2 F20 (TEM) FEI Tecnai G2 F20 TWIN TEM FEI Tecnai F20 Tecnai F20 TEM Tecnai F20

3 protocols using fei tecnai f20 tem

Characterization of Disperse Dye Formulations

Check if the same lab product or an alternative is used in the 5 most similar protocols

It was prepared by combining
carbon black (CB, 25.0 g), DE-22 (9.5 g), and deionized water (65.5
g) and continuously grinding (2000 rpm) for 120 min.
The liquid
Disperse Blue 79 (DB-79), Disperse Orange 288 (DO-288), and Disperse
Violet 93 (DV-93) were prepared in the same way as DB.
The rheology
of liquid dyes was tested on a Rheolab QC rotational
rheometer (Austria Anton-Paar China Co., Ltd., Shanghai, China). The
test conditions were 0 # rotor, temperature 20 °C, and shear
rate 0.1–1000 s^–1. The shear stress (y, Pa) and shear rate (x, s^–1) were analyzed using a linear equation (eq 1), and the viscosity (y,
mPa·s) and shear rate (x, s^–1) were analyzed using the Morgan–Mercer–Florin model
(eq 2). The transmission
electron microscopy (TEM)
was tested on an H-9500E TEM (Hitachi, Ltd., Tokyo, Japan) or an FEI
Tecnai F20 TEM (FEI, Ltd.). The purpose is to observe the interaction
of the dye, carbon black, and dispersant.

Ai L., Li S., Cao H, & Zhu Y. (2023). Conductive Properties of Polyester/Spandex Fabrics Using Liquid Carbon Black and Disperse Black Dye. ACS Omega, 8(4), 4106-4115.

+ Open protocol

+ Expand

Structural Characterization of hAire and mAire CARD Domains

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Carbon-coated hexagonal mesh copper grids (Electron Microscopy Sciences) were negatively glow-discharged for 30–45 s at 15 mA. Purified recombinant hAire and mAire CARD variants were diluted in TNE buffer (100 mM Tris pH 8, 50 mM NaCl, 10 mM EDTA) to a final concentration of ~50–200 µg/ml and immediately applied to the glow-discharged grid (Electron Microscopy Sciences). After 30 s, sample was blotted away with Whatman paper and washed with water twice. The grid was then pre-stained with 0.75% uranyl formate (UF) for 5 s, blotted and stained with 0.75% UF for 30 s. Stained grids were blotted and aspirated dry. Grids were imaged using a FEI Tecnai G² Spirit BioTWIN transmission electron microscope (TEM). In order to obtain higher resolution images of refolded mAire CARD wild-type filaments, grids were imaged with a FEI Tecnai F20 TEM. Images collected on the F20 TEM were analyzed with the image-processing suite EMAN2^{67 (link)} and 2D-classification of the processed images was done with RELION 3.0^{68 (link)}.

Huoh Y.S., Wu B., Park S., Yang D., Bansal K., Greenwald E., Wong W.P., Mathis D, & Hur S. (2020). Dual functions of Aire CARD multimerization in the transcriptional regulation of T cell tolerance. Nature Communications, 11, 1625.

+ Open protocol

+ Expand

Electron Tomography of Root Cell Organelles

Check if the same lab product or an alternative is used in the 5 most similar protocols

The procedures for ET including sample sectioning, post-staining, and electron microscopy imaging were as previously described (Cui et al., 2019) . In brief, 14 continuous 300-nm-thick sections of 1-day-old WT GFP-SDP1 and free1 GFP-SDP1 mutant root tips not occupied by large central large vacuoles were cut and poststained using uranyl acetate and lead citrate. ET images were collected with an FEI Tecnai F20 TEM (FEI Company) operating at 200 kV. For each grid, a tilt image stack (81 images) from +60° to -60° with 1.5°i ncrements was collected, and the second tilt image stack was collected by rotating the grid by 90°. Dual-axis tomograms were calculated from pairs of image stacks with the etomo program of the IMOD software package (version 4.9.7, bio3d.colorado.edu) . The 3D models were generated using the 3dmod program of the IMOD software package.

Huang S., Liu Z., Cao W., Li H., Zhang W., Cui Y., Hu S., Luo M., Zhu Y., Zhao Q., Xie L., Gao C., Xiao S, & Jiang L. (2022). The plant ESCRT component FREE1 regulates peroxisome-mediated turnover of lipid droplets in germinating Arabidopsis seedlings. The Plant cell, 34(11).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!