Xevo tqd system

Manufactured by Waters Corporation

Sourced in United States

The XEVO TQD System is a triple quadrupole mass spectrometer designed for quantitative and qualitative analysis. It provides high sensitivity, selectivity, and speed for a wide range of applications.

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Lab products found in correlation

Acquity uplc system, by Waters Corporation (1 mentions) Acquity van guard pre column, by Waters Corporation (1 mentions) Acquity uplc h class system, by Waters Corporation (1 mentions)

Spelling variants of this product

Xevo TQD (77 citations) Acquity UPLC-Xevo TQD system (4 citations) ACQUITY Xevo™ TQD system (4 citations) ACQUITY UPLC I-Class/Xevo TQD system (3 citations) Xevo TQD MS System (3 citations) TQD TM (2 citations) XevoTM (2 citations) Xevo TQD triple quadrupole system (2 citations) Xevo TQD MS/MS system (2 citations)

5 protocols using xevo tqd system

Metabolomic Profiling via HILIC-MS/MS

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For the metabolomic analysis, a previous reported hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC MS/MS) method was applied and separation was performed on an Acquity- UPLC System (Waters Corporation, Millford, USA) by a BEH Amide column (2.1 mm × 150 μm, 1.7 μm) protected by an Acquity Van-Guard pre-column (Waters Ltd., Elstree, UK). The column temperature was maintained at 40 °C [61 (link)]. The mobile phase was a mixture of (A) ACN:H₂O, 95:5 v/v and (B) H₂O:ACN, 70:30 v/v both with a final ammonium formate buffer concentration of 10 mM. Elution was performed with a gradient program.
The MS/MS method monitors 101 multiple reaction monitoring (MRM) transition for the detection and quantitation of 101 hydrophilic metabolites comprising essential and non-essential amino acids, amines, organic acids and other small ionizable endogenous metabolites. All MS data were acquired on a XEVO TQD System (Waters Corporation, Milford, MA, USA) operating by the polarity switching mode. MS parameters were optimized for each individual analyte in terms of parent/daughter ion, dwell time, cone and collision energy (V). It has been shown that the method is sensitive, robust and efficient over a wide range of concentrations.

Kerasioti E., Veskoukis A., Virgiliou C., Theodoridis G., Taitzoglou I, & Kouretas D. (2019). The Strong Antioxidant Sheep/Goat Whey Protein Protects Against mTOR Overactivation in Rats: A Mode of Action Mimicking Fasting. Antioxidants, 8(3), 71.

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Quantitative Analysis of Imidocarb in Milk and Beef

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In order to compare the ability of using ic-ELISA
and LC–MS/MS
to add and recover imidocarb in milk and beef samples, the conditions
and methods of LC–MS/MS in this experiment are based on the
previous report.^{38 (link)} The LC–MS was
performed on Waters Xevo-TQD system (Waters, USA), equipped with an
ESI source. The analytical column used was a BEH C18 column (100 mm
× 2.1 mm i.d., 1.7 μm). The operation conditions are as
follows: flow rate, 0.3 mL/min; injection volume, 1 μL; column
temperature, 45 °C. The mobile phases were 100% acetonitrile
(A) and 0.1% formic acid in ultrapure water (v/v) (B): 0 min, 95%
B; 12 min, 79% B; 15 min, 40% B; 17 min, 10% B. All chromatographic
separation processes are carried out under a gradient elution program.
The MS detection was performed by electrospray ionization in positive-ion
mode (ESI^–). The ions were detected in multiple
reaction monitoring. The parameters are set as follows: the ion source
block temperature, 100 °C; capillary voltage, 4500 V; desolvation
gas temperature, 400 °C; desolvation gas flow, 700 L/h; the cone
voltage, 30 V; the collision gas volume, 0.15 mL/min; and the [M –
H]⁺ at m/z 349 was the
parent ion of imidocarb. The daughter ion at m/z 349 → 188 was used as the quantification transition,
the daughter ion m/z 349 →
162 was selected as a qualitative ion, and the collision energy was
6 and 20, respectively.

Xu X., Wang Z., Guo L., Xu X., Wu A., Kuang H., Sun L., Song S, & Xu C. (2021). Sensitive Lateral Flow Immunoassay for the Residues of Imidocarb in Milk and Beef Samples. ACS Omega, 6(4), 2559-2569.

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Quantitative Analysis of cGAS Activity

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cGAS activity was analyzed as described previously (24 (link)). The enzymatic activity of cGAS was measured with purified recombinant full-length cGAS in 100 μl reaction buffer (2 mM cGAS, 0.2 mM ATP, 0.2 mM GTP, 100 mM NaCl, 40 mM Tris-HCl pH 7.5, 1 mM MgCl₂ and 0.01 mg•ml⁻¹ dsDNA). Then the reaction buffer was heated at 99°C to denature proteins. After centrifugation at 12,000 g for 10 min, the supernatants were collected and extracted with ACQUITYUPLC^® BEH Amide Colum, which was previously equilibrated with running buffer (1% Formic acid solution). All samples were analyzed using a Waters XEVO^® TQD system (Waters Corp.) equipped with electrospray ionization (ESI).

Chu L., Li C., Li Y., Yu Q., Yu H., Li C., Meng W., Zhu J., Wang Q., Wang C, & Cui S. (2021). Perillaldehyde Inhibition of cGAS Reduces dsDNA-Induced Interferon Response. Frontiers in Immunology, 12, 655637.

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Quantification of Bioactive Compounds in Rubia

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The quantification of Q3, Q4, Q11, Q12, Q17, Q18, Q19, and Q20 was carried out using UPLC-MS/MS. The analyses were performed using a Waters ACQUITY^®UPLC H Class system (Waters, Milford, MA, USA) fitted with a Waters XEVO^®TQD system (Milford, MA, USA) equipped with electrospray ionization (ESI). The conditions for UPLC and MS analyses were performed according to our previous method [8 (link)]. The eight compounds in the samples were identified by comparing their retention time and mass spectra with the standards, which were isolated from Rubia species in our laboratory. The quantitative data were calculated based on the calibration curves of the individual standards through plotting the MS peak areas versus the corresponding concentrations of each representative standard.

Zhang R., Miao Y., Chen L., Yi S, & Tan N. (2022). De Novo Transcriptome Analysis Reveals Putative Genes Involved in Anthraquinone Biosynthesis in Rubia yunnanensis. Genes, 13(3), 521.

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Serum Progesterone Measurement Protocol

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The venous blood samples from all participants were collected after fasting for more than 8 h at night, and placed at -80℃ before analysis. Liquid chromatography-tandem mass spectrometry (LC–MS/MS) (Waters XEVO TQD system (Waters, Milford, MA, USA)) was used to detect the concentrations of serum progesterone. For the purpose of quality control, 12 samples were tested for each batch; in addition, 1 blank sample and 1 control sample were tested before each batch. Moreover, the value of 1/2 limit of detection (LOD) was used for analysis when the sample serum progesterone level was lower than limit of quantitation (LOQ) [40 (link)]. Furthermore, the individuals of the present study were divided into three groups according to the tertile (T) of serum progesterone, and the lowest level (T1) was deemed as reference.

Wang L., Mao Z., Liu X., Wei D., Liu P., Nie L., Fan K., Kang N., Song Y., Xu Q., Wang J., Wang M., Liao W., Jing T., Li W., Wang C, & Huo W. (2021). Combined effects of progesterone and SOCS3 DNA methylation on T2DM: a case–control study. Clinical Epigenetics, 13, 181.

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