Scanning electron microscopy (SEM) of Calu-3 – S. aureus co-cultures were performed as previously described (Starner et al., 2006 (link)). Briefly, samples were fixed in 1% osmium tetroxide (EMS) dissolved in perfluorocarbon (Fluorinert FC-72; 3M, St. Paul, MN) for 2 hours at room temperature, then dehydrated by three washes with 100% ethanol for 15 minutes each. Samples were next washed in hexamethyldislazane (Ted Pella, Inc.; Redding, CA) twice for 15 minutes each, dried overnight and mounted on stubs before coating with argon, and imaged with a Hitachi S-4000 scanning electron microscope. Calu-3 cells are known to produce a thick mucus layer, which was visible in the SEM and obstructed viewing of the bacteria. Fields of view were selected on the edges where S. aureus cells were visible.
S 4000 scanning electron microscope
Manufactured by Hitachi
Sourced in Japan
The S-4000 scanning electron microscope is a high-performance instrument designed for advanced materials analysis. It utilizes a focused electron beam to scan the surface of a sample, providing detailed images with high magnification and resolution. The S-4000 is capable of resolving features down to the nanometer scale, making it a valuable tool for researchers and scientists in various fields of study.
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Lab products found in correlation
4 protocols using s 4000 scanning electron microscope
1
SEM Imaging of Calu-3 and S. aureus
2
SEM Analysis of BMSC Morphology on Acellular Dermal Matrix
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The morphology of BMSCs grown on fetal bovine acellular dermal matrix in basal medium and neural differentiation medium was examined using scanning electron microscopy after the cells were cultured for 12–34 days. The fetal bovine acellular dermal matrices with attached cells were rinsed three times with PBS and fixed in 2% buffered glutaraldehyde solution for 24 hours. Next, they were rinsed three times with 0.1 mol/L hydroxyethyl piperazine ethanesulfonic acid buffer for 5 minutes each, soaked in 1–2% osmium tetroxide for 1 hour, and rinsed three more times with 0.1 mol/L hydroxyethyl piperazine ethanesulfonic acid buffer. They were then dehydrated in increasing ethanol concentration baths (50%, 70%, 95%, 100%) twice for 10 minutes each. Finally, the scaffolds with attached cells were treated with hexamethyldisilazane and air-dried in a fume hood overnight. The dried samples were mounted on aluminum stubs, sputter-coated with 20 nm of gold-palladium, and examined using an S-4000 scanning electron microscope (Hitachi, Tokyo, Japan).
3
Nanoparticle Characterization via DLS and SEM
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The nanoparticle size distribution and polydispersity was obtained by loading aqueous solutions of the nanoparticle formulation 1 NP10 into disposable micro-cuvettes and measuring the dynamic light scattering (DLS) of the solution using a Zetasizer Nanoseries spectrometer (Malvern). For the Scanning Electron Microscope (SEM) studies, an aliquot of 1 NP10 in MilliQ water was allowed to evaporate on a square glass slide and coated with platinum. Imaging was conducted using a Hitachi S4000 Scanning Electron Microscope within the University of Leicester Advanced Microscopy Facility.
4
SEM Characterization of SWCNTs
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Scanning electron microscopy (SEM) analyses were performed by using a Hitachi S-4000 Scanning Electron Microscope (Tokyo, Japan). A few microliters of sample dispersion of SWCNTs were deposited on Silicon substrate and an ultra-thin coating of gold was applied to increase the signal-to-noise ratio. SEM images were analyzed by ImageJ [34 ,35 (link)].
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