Qiaamp dna blood kit or tissue kit

Manufactured by Qiagen

Sourced in Germany

The QIAamp DNA blood kit or tissue kit is a laboratory equipment product manufactured by Qiagen. The kit is designed to isolate and purify DNA from blood or tissue samples. It provides a simple and efficient method for extracting high-quality DNA that can be used in various downstream applications.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

QIAamp Blood Kit (194 citations) QIAamp kit (104 citations) QIAamp Tissue Kit (88 citations) DNA blood kit (56 citations) Blood & Tissue Kit (55 citations) DNA kits (7 citations) Blood kits (3 citations)

7 protocols using qiaamp dna blood kit or tissue kit

DNA Extraction from Blood and Saliva

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA was extracted from peripheral blood or saliva from each SCZ, ASD, and control participant. For DNA extraction, we used the QIAamp DNA Blood Kit or Tissue Kit (Qiagen Ltd. Hilden, Germany). The quantity of extracted DNA was estimated using the Qubit^® dsDNA BR Assay Kit (Life Technologies, Carlsbad, CA, USA) on a Qubit^® 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) following the manufacturer’s recommended protocol.

Ishizuka K., Kimura H., Wang C., Xing J., Kushima I., Arioka Y., Oya-Ito T., Uno Y., Okada T., Mori D., Aleksic B, & Ozaki N. (2016). Investigation of Rare Single-Nucleotide PCDH15 Variants in Schizophrenia and Autism Spectrum Disorders. PLoS ONE, 11(4), e0153224.

+ Open protocol

+ Expand

Genomic DNA Extraction from Blood and Saliva

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genomic DNA was extracted from peripheral blood or saliva from each SCZ, ASD and
control participant using the QIAamp DNA Blood Kit or Tissue Kit (Qiagen, Hilden,
Germany). The quantity of extracted DNA was estimated using the Qubit dsDNA BR
Assay Kit (Life Technologies, Carlsbad, CA, USA) on a Qubit 2.0 Fluorometer (Life
Technologies) following the manufacturer’s recommended protocol.

Ishizuka K., Fujita Y., Kawabata T., Kimura H., Iwayama Y., Inada T., Okahisa Y., Egawa J., Usami M., Kushima I., Uno Y., Okada T., Ikeda M., Aleksic B., Mori D., Someya T., Yoshikawa T., Iwata N., Nakamura H., Yamashita T, & Ozaki N. (2017). Rare genetic variants in CX3CR1 and their contribution to the increased risk of schizophrenia and autism spectrum disorders. Translational Psychiatry, 7(8), e1184-.

+ Open protocol

+ Expand

Targeted Genomic Profiling from Whole Blood

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genomic DNA was extracted from whole blood or saliva using the Qiagen QIAamp DNA blood kit or tissue kit (Qiagen, Hilden, Germany). Custom amplification primers were designed to span coding exons and flanking intron regions of the selected genes (transcription ID of RTN4R: ENST00000043402 from ensemble database; human reference sequence NCBI built 37) using the Ion AmpliSeq Designer (Thermo Fisher Scientific, Waltham, MA, USA). Sample amplification and equalization were achieved using Ion AmpliSeq Library Kits 2.0 and the Ion Library Equalizer Kit, respectively (Thermo Fisher Scientific). Amplified sequences were ligated with Ion Xpress Barcode Adapters (Thermo Fisher Scientific). Emulsion PCR and subsequent enrichment were performed using the Ion OneTouch Template Kit v2.0 on Ion OneTouch 2 and Ion OneTouch ES, respectively (Thermo Fisher Scientific). The final product was then sequenced on the Ion PGM sequencing platform (Thermo Fisher Scientific). Raw data output from the sequencer with the default setting; call quality ⩾20, read depth ⩾10 was uploaded to the Torrent Server (Life Technologies, Carlsbad, CA, USA) for variant calling with NCBI GRCh37 as a reference. The resulting VCF files were analyzed by Ingenuity Variant Analysis (Qiagen) for annotation and visualization.

Kimura H., Fujita Y., Kawabata T., Ishizuka K., Wang C., Iwayama Y., Okahisa Y., Kushima I., Morikawa M., Uno Y., Okada T., Ikeda M., Inada T., Branko A., Mori D., Yoshikawa T., Iwata N., Nakamura H., Yamashita T, & Ozaki N. (2017). A novel rare variant R292H in RTN4R affects growth cone formation and possibly contributes to schizophrenia susceptibility. Translational Psychiatry, 7(8), e1214-.

+ Open protocol

+ Expand

Whole Exome Sequencing of Autism Spectrum Disorder

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genomic DNA was extracted from whole blood or saliva using a Qiagen QIAamp DNA blood kit or tissue kit (Qiagen, Hilden, Germany). Raw WES data were generated and processed to BAM files at three sites (Table S1): The Broad Institute (ASD = 256, HC = 299) [11 (link)] using Illumina HiSeq sequencers and an Illumina Nextera exome capture kit; Yokohama City University (ASD = 51) [14 (link)] using Illumina HiSeq with Agilent Sure Select v5; and Nagoya University (ASD = 2) using Illumina HiSeq with Agilent Sure Select v5. Detailed descriptions of each sequencing method are presented elsewhere [11 (link), 14 (link), 19 (link)]. Each sample’s sequencing reads were aligned onto the human genome build 37 (GRCh37/hg19) and then aggregated into a BAM file. Genomic variant call format (gVCF) files were generated using Haplotype Caller, version 4.1, in the Genome Analysis Toolkit (GATK) [20 (link)]. SNVs and insertions/deletions (indels) were jointly called across all samples (ASD = 309, HC = 299) using the GenotypeGVCF function.

Kimura H., Nakatochi M., Aleksic B., Guevara J., Toyama M., Hayashi Y., Kato H., Kushima I., Morikawa M., Ishizuka K., Okada T., Tsurusaki Y., Fujita A., Miyake N., Ogi T., Takata A., Matsumoto N., Buxbaum J., Ozaki N, & Sebat J. (2022). Exome sequencing analysis of Japanese autism spectrum disorder case-control sample supports an increased burden of synaptic function-related genes. Translational Psychiatry, 12, 265.

+ Open protocol

+ Expand

OLIG2 Gene Resequencing by Next-Gen Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols

We used the QIAamp DNA blood kit or tissue kit (QIAGEN Ltd., Hilden, Germany) to extract genomic DNA from whole blood or saliva. We then used next-generation sequencing (NSC) technology on the Ion Torrent PGM^TM to re-sequence the OLIG2 coding regions (Ensembl Transcript ID: ENST00000333337.3; Chr 21: 34,398,243-34,401,504;323 amino acids) according to protocols identified in the Ion AmpliSeq^TM Library Preparation User Guide (Thermo Fisher Scientific, Waltham, MA, USA) and the Ion PGM^TM Sequencing 200 Kit (Thermo Fisher Scientific). We first targeted specific PCR amplification and then prepared libraries to obtain 200-bp PCR fragments flanked by adaptor and barcode sequences; these sequences allowed sequencing and sample identification, respectively. The concentration of each library was determined using the Ion Library TaqMan Quantitation Assay Kit (Thermo Fisher Scientific). Amplified libraries were subjected to emulsion PCR and subsequent enrichment for template-positive Ion Sphere^TM particles (ISPs) using the Ion OneTouch^TM system (Life Technologies, Carlsbad, CA, USA). ISPs were enriched and sequenced in a 200-bp configuration run using 318 chips (Life Technologies).

Furuta S., Aleksic B., Nawa Y., Kimura H., Kushima I., Ishizuka K., Kato H., Toyama M., Arioka Y., Mori D., Morikawa M., Inada T, & Ozaki N. (2022). Investigation of OLIG2 as a candidate gene for schizophrenia and autism spectrum disorder. Nagoya Journal of Medical Science, 84(2), 260-268.

+ Open protocol

+ Expand

Ion Torrent Targeted DNA Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genomic DNA was extracted from whole blood or saliva using the QIAGEN QIAamp DNA blood kit or tissue kit (QIAGEN Ltd., Germany). Custom amplification primers were designed to cover coding exons and flanking intron regions of the selected genes with Ion AmpliSeq Designer (Thermo Fisher Scientific, USA). Sample amplification and equalization were achieved using Ion AmpliSeq Library Kits 2.0 and the Ion Library Equalizer Kit, respectively (Thermo Fisher Scientific, USA). Amplified sequences were ligated with Ion Xpress Barcode Adapters (Thermo Fisher Scientific, USA). Emulsion PCR and subsequent enrichment were performed using the Ion OneTouch Template Kit v2.0 on Ion OneTouch 2 and Ion OneTouch ES, respectively (Thermo Fisher Scientific, USA). The final product was then sequenced on the Ion PGM sequencing platform (Thermo Fisher Scientific, USA). Raw data output from the sequencer was deposited in the DNA Data Bank of Japan (DDBJ) (http://www.ddbj.nig.ac.jp) under the accession number DRA004490, and uploaded to the Torrent Server (Life Technologies, USA) for variant calling, with NCBI GRCh37 as a reference. The resulting VCF files were analyzed by Ingenuity Variant Analysis (QIAGEN Ltd., Germany) for annotation and visualization.

Xing J., Kimura H., Wang C., Ishizuka K., Kushima I., Arioka Y., Yoshimi A., Nakamura Y., Shiino T., Oya-Ito T., Takasaki Y., Uno Y., Okada T., Iidaka T., Aleksic B., Mori D, & Ozaki N. (2016). Resequencing and Association Analysis of Six PSD-95-Related Genes as Possible Susceptibility Genes for Schizophrenia and Autism Spectrum Disorders. Scientific Reports, 6, 27491.

+ Open protocol

+ Expand

Next-Generation Sequencing of Genomic DNA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genomic DNA was extracted from whole blood or saliva using the QIAGEN QIAamp DNA blood kit or tissue kit (QIAGEN, Hilden, Germany). Custom amplification primers were designed, using Ion AmpliSeq Designer (Thermo Fisher Scientific, Waltham, MA, USA), to cover coding exons and flanking intron regions of the selected genes. Sample amplification and equalization were achieved using Ion AmpliSeq Library Kits 2.0 and the Ion Library Equalizer Kit, respectively (Thermo Fisher Scientific). Amplified sequences were ligated with Ion Xpress Barcode Adapters (Thermo Fisher Scientific). Emulsion PCR and subsequent enrichment were performed using the Ion OneTouch Template Kit v2.0 on Ion OneTouch 2 and Ion OneTouch ES, respectively (Thermo Fisher Scientific). The final product then was sequenced on the Ion PGM sequencing platform (Thermo Fisher Scientific). Raw data output from the sequencer was deposited in the DNA Data Bank of Japan (DDBJ) (http://www.ddbj.nig.ac.jp) under Accession Number DRA004490, and uploaded to the Torrent Server (Thermo Fisher Scientific) for variant calling, with NCBI GRCh37 as a reference. The resulting VCF (variant call format) files were analyzed by Ingenuity Variant Analysis (QIAGEN) for annotation and visualization. Combined Annotation Dependent Depletion (http://cadd.gs.washington.edu/) was applied for annotation of genetic variants.

Wang C., Horigane S.I., Wakamori M., Ueda S., Kawabata T., Fujii H., Kushima I., Kimura H., Ishizuka K., Nakamura Y., Iwayama Y., Ikeda M., Iwata N., Okada T., Aleksic B., Mori D., Yoshida T., Bito H., Yoshikawa T., Takemoto-Kimura S, & Ozaki N. (2022). Identification of ultra-rare disruptive variants in voltage-gated calcium channel-encoding genes in Japanese samples of schizophrenia and autism spectrum disorder. Translational Psychiatry, 12, 84.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!