Animals were immobilized on 2% agarose pads with 30 mM levamisole. Fluorescence imaging was performed at the Queensland Brain Institute’s Advanced Microscopy Facility using a spinning-disk confocal system (3i Yokogawa W1 SDC) controlled by Slide-book 6.0 software. Animals were imaged with an Olympus 100× 1.4 NA Plan-Apochromat objective. Z series of optical sections were acquired at 0.11 μm steps. Images were deconvolved with Huygens Professional version 16.10 (Scientific Volume Imaging, the Netherlands) and then processed to yield maximum intensity projections using ImageJ 1.51n (Wayne Rasband, National Institutes of Health) (Schneider et al., 2012 (link)).
1.4 na plan apochromat objective
Manufactured by Olympus
The 100× 1.4 NA Plan-Apochromat objective is a high-performance lens designed for advanced microscopy applications. It offers a numerical aperture of 1.4, which provides excellent light-gathering capability and high-resolution imaging. The Plan-Apochromat design ensures superior optical performance with reduced chromatic and spherical aberrations.
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Lab products found in correlation
6 protocols using 1.4 na plan apochromat objective
1
Spinning-Disk Confocal Imaging of Immobilized Animals
2
Spinning-Disk Confocal Imaging of Immobilized Animals
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Animals were immobilized on 2% agarose pads with 30 mM levamisole. Fluorescence imaging was performed at the Queensland Brain Institute’s Advanced Microscopy Facility using a spinning-disk confocal system (3i Yokogawa W1 SDC) controlled by Slide-book 6.0 software. Animals were imaged with an Olympus 100× 1.4 NA Plan-Apochromat objective. Z series of optical sections were acquired at 0.11 μm steps. Images were deconvolved with Huygens Professional version 16.10 (Scientific Volume Imaging, the Netherlands) and then processed to yield maximum intensity projections using ImageJ 1.51n (Wayne Rasband, National Institutes of Health) (Schneider et al., 2012 (link)).
3
Confocal Imaging of Immobilized Worms
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Animals were immobilized on 2% agarose pads with 20 mM levamisole. Fluorescence imaging was performed on a spinning-disk confocal system (3i Yokogawa W1 SDC, Evolve EMCCD; Photometrics) controlled by Slidebook 6.0 software. Animals were imaged with an Olympus 100× 1.4 NA Plan-Apochromat objective at RT. A Z series of optical sections was acquired in 0.13-µm steps. Images were deconvolved with Huygens Professional version 16.10 (Scientific Volume Imaging) and processed to yield maximum-intensity projections by using ImageJ version 1.53a (Wayne Rasband, National Institutes of Health).
4
Spinning-Disk Confocal Imaging of C. elegans
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Animals were immobilized on 2% agarose pads with 30 mM levamisole. Fluorescence imaging was performed on a spinning-disk confocal system (3i Yokogawa W1 SDC) controlled by Slidebook 6.0 software. Animals were imaged with an Olympus 100 × 1.4 NA Plan-Apochromat objective. Z series of optical sections were acquired at 0.11 μm steps. Images were deconvolved with Huygens Professional version 16.10 (Scientific Volume Imaging, The Netherlands) and then processed to yield maximum intensity projections using ImageJ 1.51 n (Wayne Rasband, National Institutes of Health).
5
Fluorescence Imaging of Immobilized Nematodes
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Animals were immobilized on 2% agarose pads with 30 mM levamisole. Fluorescence imaging was performed on a spinning-disk confocal system (3i Yokogawa W1 SDC) controlled by Slidebook 6.0 software. Animals were imaged with an Olympus 100× 1.4 NA Plan-Apochromat objective. Z series of optical sections were acquired at 0.11 μm steps. Images were deconvolved with Huygens Professional version 16.10 (Scientific Volume Imaging, the Netherlands) and then processed to yield maximum intensity projections using ImageJ 1.51n (Wayne Rasband, National Institutes of Health) (Schneider et al., 2012 (link)).
6
Fluorescence Imaging of Immobilized Nematodes
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